Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » DDIT4 antibody

DDIT4 antibody

(reset)

Principal name

DDIT4 antibody

Alternative names for DDIT4 antibody

DNA-damage-inducible transcript 4, DDIT-4, REDD1, REDD-1, RTP801, HIF-1 responsive protein, Dig2, Protein regulated in development and DNA damage response 1

SwissProt ID

Q08E62 (Bovin), Q8VHZ9 (Rat), Q9D3F7 (Mouse), Q9NX09 (Human)

Gene ID

54541 (DDIT4), 74747 (Ddit4), 140942

Ncbi ID

9606, NP_061931.1, NP_061931, NM_029083, NM_080906, NM_019058, NP_083359.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of DDIT4 antibody

REDD1 is a novel transcriptional target of p53 induced following DNA damage. During embryogenesis, REDD1 expression mirrors the tissue-specific pattern of the p53 family member p63, and TP63 null embryos show virtually no expression of REDD1, which is restored in mouse embryo fibroblasts following p63 expression. In differentiating primary keratinocytes, TP63 and REDD1 expression are coordinately downregulated, and ectopic expression of either gene inhibits in vitro differentiation. REDD1 appears to function in the regulation of reactive oxygen species (ROS); TP63 null fibroblasts have decreased ROS levels and reduced sensitivity to oxidative stress, which are both increased following ectopic expression of either TP63 or REDD1. Thus, REDD1 encodes a shared transcriptional target that implicates ROS in the p53-dependent DNA damage response and in p63-mediated regulation of epithelial differentiation.

General readings

Yoshida T., et al. (2010) Nat Med. 16(7):767-73.
Regazzetti C., et al. (2010) J Biol Chem. 285(8):5157-64.

H00054541-B01 - DDIT4
Western Blot analysis of DDIT4 expression in transfected 293T cell line (H00054541-T01) by DDIT4 MaxPab polyclonal antibody.Lane 1: DDIT4 transfected lysate(25.52 KDa).Lane 2: Non-transfected lysate.
H00054541-B01P - DDIT4
Western Blot analysis of DDIT4 expression in transfected 293T cell line (H00054541-T01) by DDIT4 MaxPab polyclonal antibody.Lane 1: DDIT4 transfected lysate(25.52 KDa).Lane 2: Non-transfected lysate.
AP13249PU-N - DDIT4
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with DDIT4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP13249PU-N - DDIT4
Western blot analysis of DDIT4 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). DDIT4 (arrow) was detected using the purified Pab (1:60 dilution).
PAB13343 - DDIT4
Immunohistochemistry of DDIT4 in mouse kidney tissue with DDIT4 polyclonal antibody ( Cat # PAB13343 ) at 5 µg/ml.
PAB13343 - DDIT4
Western blot analysis of DDIT4 in human kidney tissue lysate with DDIT4 polyclonal antibody ( Cat # PAB13343 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
PAB13346 - DDIT4
Western blot analysis of DDIT4 in 293 cell lysate with DDIT4 polyclonal antibody ( Cat # PAB13346 ) at ( A ) 2 and ( B ) 4 µg/ml.
AR09836PU-L - DDIT4
AR09836PU-L - DDIT4
Recombinant human DDIT4, 1-232 aa, His-tagged
TP302847 - DDIT4
TA306539 - DDIT4
Western blot analysis of RTP801 in human kidney tissue lysate with RTP801 antibody at (A) 0.5 and (B) 1 ug/ml.
TA306539 - DDIT4
Immunohistochemistry of RTP801 in mouse kidney tissue with RTP801 antibody at 5 ug/ml.
TA306543 - DDIT4
Western blot analysis of RTP801 in 293 cell lysate with RTP801 antibody at (A) 2 and (B) 4 µg/ml.
TA344821 - DDIT4
Host: Rabbit; Target Name: Ddit4; Sample Tissue: Rat Thymus lysates; Antibody Dilution: 1.0 ug/ml
TA349879 - DDIT4
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (DDIT4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA349879 - DDIT4
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using (DDIT4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DDIT4 (Center) antibody
  Rabbit Polyclonal RTP801 Antibody  
Western blot analysis of RTP801 in human kidney tissue lysate with RTP801 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

DDIT4 (N-term) antibody
  Rabbit Polyclonal RTP801 Antibody  
Western blot analysis of RTP801 in 293 cell lysate with RTP801 antibody at (A) 2 and (B) 4 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.

DDIT4 antibody
  Rabbit Polyclonal Anti-DDIT4 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (DDIT4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

DDIT4 (N-term) antibody
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with DDIT4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DDIT4
  Recombinant protein of human DNA-damage-inducible transcript 4 (DDIT4)  
DDIT4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ddit4 (GFP-tagged ORF) - Rat DNA-damage-inducible transcript 4 (Ddit4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Ddit4 (GFP-tagged ORF) - Rat DNA-damage-inducible transcript 4 (Ddit4), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Ddit4 (Myc-DDK-tagged ORF) - Rat DNA-damage-inducible transcript 4 (Ddit4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Ddit4 (Myc-DDK-tagged ORF) - Rat DNA-damage-inducible transcript 4 (Ddit4), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

7 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (4)
  2. Protein | Growth Factor (1)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
Target
  1. DDIT4 (5)
Property
  1. Center (1)
  2. N-term (2)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (3)
Applications
  1. Enzyme Immunoassay (E) (3)
  2. Paraffin Sections (P) (3)
  3. Western blot / Immunoblot (WB) (3)
Presentation
  1. Aff - Purified (2)
  2. Purified (2)
Host
  1. Rabbit (4)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. IHC (2)
  3. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (7)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn