DDOST antibody
Principal name
DDOST antibody
Alternative names for DDOST antibody
KIAA0115, OST48, WBP1, AGE-R1, Oligosaccharyl transferase 48 kDa subunit, Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit
SwissProt ID
A6QPY0 (Bovin), O54734 (Mouse), P39656 (Human), P48440 (Chick), Q29381 (Pig), Q5R501 (Ponab), Q641Y0 (Rat)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of DDOST antibody
- Kit Component:
- - KN200672G1, DDOST gRNA vector 1 in pCas-Guide vector
- KN200672G2, DDOST gRNA vector 2 in pCas-Guide vector
- KN200672D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Shibatani,T., (2005) Biochemistry 44 (16), 5982-5992
Human Jurkat; WB Suggested Anti-DDOST Antibody Titration: 5.0ug/ml. Positive Control: Jurkat cell lysate; DDOST antibody - N-terminal region (ARP46585_T100) in Human Jurkat cells using Western Blot
Western Blot analysis of DDOST expression in transfected 293T cell line (H00001650-T03) by DDOST MaxPab polyclonal antibody.Lane 1: DDOST transfected lysate(50.70 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western blot analysis of A549 cell lysate (35ug) by using anti-DDOST monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDOST (RC200672, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDOST.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
Western blot analysis of A549 cell lysate (35ug) by using anti-DDOST monoclonal antibody. Dilution: 1:500
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDOST (RC200672, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDOST.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803619)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803619)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803619)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803619)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803619)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803619)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803619)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803619)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803619)
Western blot analysis of A549 cell lysate (35ug) by using anti-DDOST monoclonal antibody. Dilution: 1:500
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDOST (RC200672, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDOST.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803634)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDOST (RC200672, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDOST.
WB Suggested Anti-DDOST Antibody Titration: 5.0 ug/ml; Positive Control: Jurkat cell lysate
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA803614 | DDOST antibody |
||||||
| DDOST mouse monoclonal antibody, clone OTI1C1 (formerly 1C1) | |||||||
 |
Mouse | IgG2a | OTI1C1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
|
||||||
| TA803618 | DDOST antibody |
||||||
| DDOST mouse monoclonal antibody, clone OTI2H2 (formerly 2H2) | |||||||
 |
Mouse | IgG1 | OTI2H2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA803619 | DDOST antibody |
||||||
| DDOST mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) | |||||||
 |
Mouse | IgG1 | OTI2H1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
||||||
| TA803634 | DDOST antibody |
||||||
| DDOST mouse monoclonal antibody, clone OTI2B4 (formerly 2B4) | |||||||
 |
Mouse | IgG1 | OTI2B4 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +13 additional images |
Highly validated antibody!
|
||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300672 | DDOST |
||||
| Recombinant protein of human dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205736L1V | Lenti ORF particles, Ddost (Myc-DDK-tagged ORF) - Rat dolichyl-diphosphooligosaccharide-protein glycosyltransferase (Ddost), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€990.00
|
|
| OriGene Technologies, Inc. | ||
