DDX27 antibody
Principal name
DDX27 antibody
Alternative names for DDX27 antibody
RHLP, HSPC259, PP3241, DEAD box protein 27, Probable ATP-dependent RNA helicase DDX27
SwissProt ID
Q96GQ7 (Human)
Gene ID
55661 (DDX27)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of DDX27 antibody
- Kit Component:
- - KN219725G1, DDX27 gRNA vector 1 in pCas-Guide vector
- KN219725G2, DDX27 gRNA vector 2 in pCas-Guide vector
- KN219725D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Deloukas, P., et al. Nature 414(6866):865-871 (2001).
Human HepG2; WB Suggested Anti-DDX27 Antibody Titration: 0.625ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate. DDX27 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; DDX27 antibody - N-terminal region (ARP36447_T100) in Human HepG2 cells using Western Blot
Human Jurkat; WB Suggested Anti-DDX27 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; DDX27 antibody - N-terminal region (ARP36448_P050) in Human Jurkat cells using Western Blot
Formalin fixed and paraffin embedded human prsotate carcinoma stained with DDX27 Antibody (C-term) Cat.-No AP51219PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining
Western blot analysis of DDX27 Antibody (C-term) Cat.-No AP51219PU-N in K562 cell line lysates (35ug/lane). This demonstrates the DDX27 antibody detected the DDX27 protein (arrow).
Flow cytometric analysis of K562 cells using DDX27 Antibody (C-term) Cat.-No AP51219PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP51219PU-N | DDX27 (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
 +2 additional images |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY413477 | DDX27 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. |