DDX5 antibody
Principal name
DDX5 antibody
Alternative names for DDX5 antibody
DDX-5, G17P1, HELR, HLR1, DEAD box protein 5, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68
Gene ID
1655 (DDX5)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of DDX5 antibody
DEAD box proteins, characterized byThe conserved motif Asp-Glu-Ala-Asp (DEAD), are putative R helicases.They are implicated in a number of cellular processes involving alteration of R secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based onTheir distribution patterns, some members ofThis family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.This gene encodes a DEAD box protein, which is a R-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting withThe simian virus 40 tumor antigen.This gene consists of 13 exons, and altertively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded byThese transcripts have been identified. [provided by RefSeq, Jul 2008].
General readings
Guil,S., et al., (2003) Mol. Cell. Biol. 23 (8), 2927-2941
Human Jurkat; WB Suggested Anti-DDX5 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. DDX5 is supported by BioGPS gene expression data to be expressed in Jurkat.; DDX5 antibody - N-terminal region (ARP36366_T100) in Human Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-DDX5 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate. DDX5 is supported by BioGPS gene expression data to be expressed in Jurkat.; DDX5 antibody - C-terminal region (ARP36365_T100) in Human Jurkat cells using Western Blot
DDX5 polyclonal antibody ( Cat # PAB6597 ) staining ( 0.5 µg/ml ) of A-431 cell lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of DDX5 antibody (C-term) in T47D cell line lysates (35ug/lane). DDX5 (arrow) was detected using the purified Pab
Immunofluorescence of purified MaxPab antibody to DDX5 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of DDX5 expression in transfected 293T cell line (H00001655-T01) by DDX5 MaxPab polyclonal antibody.Lane 1: DDX5 transfected lysate(67.54 KDa).Lane 2: Non-transfected lysate.
DDX5 MaxPab polyclonal antibody. Western Blot analysis of DDX5 expression in Raw 264.7.
DDX5 MaxPab polyclonal antibody. Western Blot analysis of DDX5 expression in Hela NE.
Western blot analysis of DDX5 antibody (Center) (AP17277PU-N) in Romas cell line lysates (35 µg/lane). DDX5 (arrow) was detected using the purified Pab.
Immunohistochemistry Image: Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry Image: Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot Image: Antibody staining (0.5 µg/ml) of A431 cell lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of DDX5 (pY593) expression in Hela (A); A431 (B) whole cell lysates.
Immunohistochemical analysis of DDX5 (pY593) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of DDX5 (pY593) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TA302658 (0.3µg/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA302658 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis of extracts of various cell lines, using DDX5 antibody.
Immunohistochemistry of paraffin-embedded human thyroid cancer using DDX5 antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded rat kidney using DDX5 antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded rat liver using DDX5 antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of U20S cell using DDX5 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-DDX5 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysateDDX5 is supported by BioGPS gene expression data to be expressed in Jurkat
WB Suggested Anti-DDX5 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateDDX5 is supported by BioGPS gene expression data to be expressed in Jurkat
Total cell lysate from 293T cells (lane 1) or NIH-3T3 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-DDX5 (clone 14B9A58) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
HeLa cells were stained with purified anti-DDX5 (14B9A58) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody and Alexa Fluor594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300371 | DDX5 |
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| Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
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