DDX56 antibody
Principal name
DDX56 antibody
Alternative names for DDX56 antibody
DDX21, NOH61, ATP-dependent 61 kDa nucleolar RNA helicase, DEAD box protein 56, Probable ATP-dependent RNA helicase DDX56, DEAD-box protein 21
Ncbi ID
NP_061955.1, 9606, NP_061955, NM_019082, NP_080814.1, NM_026538
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of DDX56 antibody
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative R helicases. They are implicated in a number of cellular processes involving alteration of R secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012].
General readings
Matsuoka S., et al (2007). Science 316:1160-1166
Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody (M05), clone 4C5.Lane 1: DDX56 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.
DDX56 monoclonal antibody (M05), clone 4C5 Western Blot analysis of DDX56 expression in HeLa ( Cat # L013V1 ).
DDX56 monoclonal antibody (M05), clone 4C5. Western Blot analysis of DDX56 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged DDX56 is approximately 0.1ng/ml as a capture antibody.
DDX56 monoclonal antibody (M03), clone 6B9. Western Blot analysis of DDX56 expression in NIH/3T3(Cat # L018V1 ).
DDX56 monoclonal antibody (M03), clone 6B9. Western Blot analysis of DDX56 expression in Raw 264.7(Cat # L024V1 ).
DDX56 monoclonal antibody (M03), clone 6B9. Western Blot analysis of DDX56 expression in HepG2 ( Cat # L019V1 ).
DDX56 monoclonal antibody (M03), clone 6B9. Western Blot analysis of DDX56 expression in PC-12 ( Cat # L012V1 ).
DDX56 monoclonal antibody (M03), clone 6B9. Western Blot analysis of DDX56 expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of DDX56 expression in transfected 293T cell line (H00054606-T01) by DDX56 MaxPab polyclonal antibody.Lane 1: DDX56 transfected lysate(60.17 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of DDX56 expression in transfected 293T cell line (H00054606-T02) by DDX56 MaxPab polyclonal antibody.Lane 1: DDX56 transfected lysate(61.60 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802815)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX56 (RC201477, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX56.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802816)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX56 (RC201477, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX56.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802817)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802817)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802817)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802817)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX56 (RC201477, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX56.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX56 (RC201477, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX56.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX56 (RC201477, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX56.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803232)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803232)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803232)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803232)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803232)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803232)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803232)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803232)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX56 (RC201477, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX56.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-DDX56 mouse monoclonal antibody (UM800049). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-DDX56 mouse monoclonal antibody (UM800050). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. TA309015 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using DDX56(TA309015) antibody at 1:500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DDX56(TA309015) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
- Quick-Filters:
- Highly Validated (7)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM800049 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody,clone UMAB152 | |||||||
 |
Mouse | IgG1 | UMAB152 | Purified | Hu | P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
 +22 additional images |
Highly validated antibody!
|
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| UM800050 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody,clone UMAB153 | |||||||
 |
Mouse | IgG1 | UMAB153 | Purified | Hu | P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +17 additional images |
Highly validated antibody!
|
||||||
| UM870049 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody,clone UMAB152 | |||||||
|
Mouse | IgG1 | UMAB152 | Purified | Hu | P, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +22 additional images |
Highly validated antibody!
|
||||||
| UM870050 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody,clone UMAB153 | |||||||
|
Mouse | IgG1 | UMAB153 | Purified | Hu | P, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +17 additional images |
Highly validated antibody!
|
||||||
| TA802815 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5) | |||||||
 |
Mouse | IgG1 | OTI2E5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +13 additional images |
Highly validated antibody!
|
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| TA802817 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody, clone OTI5C1 (formerly 5C1) | |||||||
 |
Mouse | IgG1 | OTI5C1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA803232 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody, clone OTI11H7 (formerly 11H7) | |||||||
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Mouse | IgG1 | OTI11H7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA309015 | DDX56 antibody |
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| Rabbit Polyclonal antibody to DDX56 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 56) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA802816 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody, clone OTI6E8 (formerly 6E8) | |||||||
 |
Mouse | IgG2a | OTI6E8 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA802823 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody, clone OTI5A9 (formerly 5A9) | |||||||
 |
Mouse | IgG1 | OTI5A9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA802941 | DDX56 antibody |
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| DDX56 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6) | |||||||
 |
Mouse | IgG1 | OTI1G6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301477 | DDX56 |
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| Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (DDX56) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412765 | DDX56 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208695L2V | Lenti ORF particles, Ddx56 (GFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), 200 uL, >10^7 TU/mL |
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200 µl /
€1,250.00
|
|
| OriGene Technologies, Inc. | ||
| MR208695L1V | Lenti ORF particles, Ddx56 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (Ddx56), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||