DDX58 antibody
Principal name
DDX58 antibody
Alternative names for DDX58 antibody
RIG-1, RIG-I, DKFZp434J1111, DKFZp686N19181, FLJ13599, DEAD box protein 58, Probable ATP-dependent RNA helicase DDX58, Retinoic acid-inducible gene 1 protein
Ncbi ID
9606, NP_055129, NP_766277.3, NP_055129.2, NM_014314, O95786, NM_172689
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of DDX58 antibody
Antiviral inte immunity depends on the combition of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and R helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes. TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including D, single-stranded R (ssR), double-stranded R (dsR), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection. Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsR and two amino-termil caspase recruitment domains (CARD) that are required for triggering downstream sigling. Rig-I binds both dsR and viral ssR that contains a 5'-triphosphate end not seen in host R. Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses . The CARD domain of the helicases, which is sufficient to generate sigling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-?B, TBK1/IKKe, and IRF-3/IRF-7.
24-hour post infection immunoblots of whole cell lysates from primary murine microglia cells (2x10e6) untreated (0) or exposed to vesicular stomatitis virus at a range of viral particle/cell ratios. Data courtesy of Dr. Samantha Furr, University of North Carolina at Charlotte.
Western blot analysis of DDX58 Antibody Cat.-No AP11678PU-N in 293 cell line lysates (35ug/lane). DDX58 (arrow) was detected using the purified Pab.
Immunohistochemistry of DDX58 in human heart tissue with DDX58 polyclonal antibody ( Cat # PAB12973 ) at 5 µg/ml.
Western blot analysis of DDX58 in C2C12 cell lysate with DDX58 polyclonal antibody ( Cat # PAB12973 ) at ( A ) 0.5, ( B ) 1 and ( C ) 2 µg/ml.
Western Blot analysis of DDX58 expression in transfected 293T cell line (H00023586-T01) by DDX58 MaxPab polyclonal antibody.Lane 1: DDX58 transfected lysate(101.75 KDa).Lane 2: Non-transfected lysate.
DDX58 MaxPab polyclonal antibody. Western Blot analysis of DDX58 expression in Jurkat.
Human OVCAR-3; WB Suggested Anti-DDX58 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: OVCAR-3 cell lysate; DDX58 antibody - middle region (ARP36416_P050) in Human OVCAR-3 cells using Western Blot
Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Brain, cortex, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from Raw 264.7 cells starved for 20h (lane 1) and starved for 20h and treated with 1 ug/ml LPS for 9h (lane 2) using anti-RIG-1 antibody (clone SS1A).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506141)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506141)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506141)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506141)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506141)
Anti-DDX58 mouse monoclonal antibody (TA506141) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DDX58(RC217615).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX58 (RC217615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX58.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-DDX58 monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDX58 mouse monoclonal antibody. (TA506160)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (TA506160)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (TA506160)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX58 (RC217615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX58.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506202)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506202)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506202)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506202)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX58 (RC217615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX58.
Western blot analysis of RIG-1 in C2C12 cell lysate with RIG-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunohistochemistry of RIG-1 in human heart tissue with RIG-1 antibody at 5 ug/ml.
Predicted band size: 107 kDa . Positive control: Jurkat, HepG2 and MCF7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Jurkat cell lysate Lane2: HepG2 cell lysate Lane3: MCF7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of various cell lines, using DDX58 antibody.
Immunohistochemistry of paraffin-embedded human rectal cancer using DDX58 antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of MCF-7 cell using DDX58 antibody. Blue: DAPI for nuclear staining.
Western Blot: DDX58 Antibody (2M6F10) [TA337138] - Western blot analysis of RIG-I in Jurkat cell lysate in the A) absence and B) presence of immunizing peptide and C) HEK 293 cell lysate using TA337138 at 10 ug/ml.
Immunohistochemistry: DDX58 Antibody (2M6F10) [TA337138] - Analysis of RIG-I in formalin-fixed, paraffin-embedded human kidney using this antibody at 5 ug/ml.
WB Suggested Anti-DDX58 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: OVCAR-3 cell lysate
Total cell lysates from THP-1 cells untreated (lane 1) or treated (lane 2) with 20 ng/mL PMA for 72 hours and 1 ug/mL LPS for 24 hours were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-DDX58 (clone 9F1B20) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Quick-Filters:
- Cited (1)
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA506141 | DDX58 antibody |
||||||
| DDX58 mouse monoclonal antibody, clone OTI6C1 (formerly 6C1) | |||||||
 |
Mouse | IgG2a | OTI6C1 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Top Selling product! Highly validated antibody!
|
||||||
| TA506202 | DDX58 antibody |
||||||
| DDX58 mouse monoclonal antibody, clone OTI8D2 (formerly 8D2) | |||||||
 |
Mouse | IgG1 | OTI8D2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Top Selling product! Highly validated antibody!
|
||||||
| TA506160 | DDX58 antibody |
||||||
| DDX58 mouse monoclonal antibody, clone OTI7D5 (formerly 7D5) | |||||||
 |
Mouse | IgG2b | OTI7D5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA306359 | DDX58 antibody |
||||||
| Rabbit Polyclonal RIG-1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA322385 | DDX58 antibody |
||||||
| Anti-DDX58 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA326840 | DDX58 antibody |
||||||
| DDX58 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA328097 | DDX58 (C-term) antibody |
||||||
| Rat Monoclonal anti-RIG-1 Antibody | |||||||
 |
Rat | IgG2a | SS1A | Purified | Ms | WB |
0.1 mg /
€230.00
|
| OriGene Technologies, Inc. | |||||||
| TA350942 | DDX58 antibody |
||||||
| Rabbit Polyclonal Anti-DDX58 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP11676PU-N | DDX58 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP11678PU-N | DDX58 antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP22933PU-N | DDX58 antibody |
||||||
 |
Goat | Aff - Purified | Elephant, Hu | P |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP22979PU-N | DDX58 antibody |
||||||
 |
Goat | Aff - Purified | Hu | P |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN217615 | DDX58 - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP317615 | DDX58 |
||||
| Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Published recently! |
|||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY415370 | DDX58 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |