DDX6 antibody
Principal name
DDX6 antibody
Alternative names for DDX6 antibody
DDX-6, HLR2, RCK, Oncogene RCK, DEAD box protein 6, ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54
Ncbi ID
9606, NP_004388, NP_004388.2, NP_001104296.1, NM_001110826, NM_007841, NM_004397
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of DDX6 antibody
- Kit Component:
- - KN304411G1, Ddx6 gRNA vector 1 in pCas-Guide vector
- KN304411G2, Ddx6 gRNA vector 2 in pCas-Guide vector
- KN304411D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Broytman, O., et al. Neurobiol. Aging 30(12):1962-1974 (2009).
Han, J.W., et al. Nat. Genet. 41(11):1234-1237 (2009).
Minshall, N., et al. Mol. Biol. Cell 20(9):2464-2472 (2009).
Tritschler, F., et al. Mol. Cell 33(5):661-668 (2009).
Immunoperoxidase of monoclonal antibody to DDX6 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Western blot analysis of DDX6 over-expressed 293 cell line, cotransfected with DDX6 Validated Chimera RNAi ( Cat # H00001656-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DDX6 monoclonal antibody (M01), clone 3D2 (Cat # H00001656-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of DDX6 expression in transfected 293T cell line by DDX6 monoclonal antibody (M01), clone 3D2.Lane 1: DDX6 transfected lysate(53.2 KDa).Lane 2: Non-transfected lysate.
DDX6 monoclonal antibody (M01), clone 3D2 Western Blot analysis of DDX6 expression in Hela ( Cat # L013V1 ).
Human HepG2; WB Suggested Anti-DDX6 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; DDX6 antibody - N-terminal region (ARP36368_T100) in Human HepG2 cells using Western Blot
HEK293 overexpressing human DDX6 and probed with DDX6 polyclonal antibody ( Cat # PAB6971 ) ( mock transfection in first lane ), tested by Origene.
DDX6 polyclonal antibody ( Cat # PAB6971 ) ( 0.3 µg/ml ) staining of Jurkat cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
DDX6 MaxPab rabbit polyclonal antibody. Western Blot analysis of DDX6 expression in mouse testis.
DDX6 MaxPab rabbit polyclonal antibody. Western Blot analysis of DDX6 expression in human liver.
Western Blot analysis of DDX6 expression in transfected 293T cell line by DDX6 MaxPab rabbit polyclonal antibody.Lane 1: DDX6 transfected lysate(53.20 KDa).Lane 2: Non-transfected lysate.
Human OVCAR-3; WB Suggested Anti-DDX6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: OVCAR-3 cell lysate. DDX6 is supported by BioGPS gene expression data to be expressed in OVCAR3.; DDX6 antibody - N-terminal region (ARP36367_P050) in Human OVCAR-3 cells using Western Blot
Formalin fixed, paraffin embedded breast carcinoma reacted with DDX6 Antibody (Center) Cat.-No AP51226PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysism of DDX6 Antibody (Center) Cat.-No AP51226PU-N in Hela,MCF-7 cell line lysates (35ug/lane).This demonstrates the DDX6 antibody detected the DDX6 protein (arrow).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDX6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804323)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX6 (RC209431, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX6.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX6 (RC209431, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX6.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX6 (RC209431, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX6.
TA302462 (0.3 ug/ml) staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-DDX6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: OVCAR-3 cell lysateDDX6 is supported by BioGPS gene expression data to be expressed in OVCAR3
WB Suggested Anti-DDX6 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Total cell lysate from Jurkat cells (lane 1), HeLa cells (lane 2), and mouse NIH-3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-DDX6 clone (11G10B39) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
A431 cells were stained with purified anti-DDX6 clone (11G10B39) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG antibody and Alexa Fluorreg; 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302462 | DDX6 antibody |
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| Goat Polyclonal Antibody against DEAD-box protein 6 | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA804323 | DDX6 antibody |
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| DDX6 mouse monoclonal antibody, clone OTI2D6 (formerly 2D6) | |||||||
 |
Mouse | IgG2a | OTI2D6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|
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| TA804349 | DDX6 antibody |
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| Purified DDX6 mouse monoclonal antibody, clone OTI3B7 (formerly 3B7) | |||||||
 |
Mouse | IgG2b | OTI3B7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804432 | DDX6 antibody |
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| Purified DDX6 mouse monoclonal antibody, clone OTI3E2 (formerly 3E2) | |||||||
 |
Mouse | IgG1 | OTI3E2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP51226PU-N | DDX6 (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309431 | DDX6 |
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| Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207739L2V | Lenti ORF particles, Ddx6 (GFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (Ddx6), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,170.00
|
|
| OriGene Technologies, Inc. | ||
| MR207739L1V | Lenti ORF particles, Ddx6 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (Ddx6), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,040.00
|
|
| OriGene Technologies, Inc. | ||