DENND1B antibody
Principal name
DENND1B antibody
Alternative names for DENND1B antibody
C1orf218, FAM31B, Connecdenn 2, DENN domain-containing protein 1B
Ncbi ID
9606, NP_659414, NP_001182144, NP_001182144.1, NM_144977, NP_851992.1, NM_001166501, NM_001195215
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of DENND1B antibody
- Kit Component:
- - KN304488G1, BC042698 gRNA vector 1 in pCas-Guide vector
- KN304488G2, BC042698 gRNA vector 2 in pCas-Guide vector
- KN304488D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ota,T., (2004) Nat. Genet. 36 (1), 40-45
Western Blot analysis of DENND1B expression in transfected 293T cell line (H00163486-T01) by DENND1B MaxPab polyclonal antibody.Lane 1: DENND1B transfected lysate(43.56 KDa).Lane 2: Non-transfected lysate.
Human Muscle; WB Suggested Anti-DENND1B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; DENND1B antibody - N-terminal region (ARP52770_P050) in Human Muscle cells using Western Blot
Human 293T; WB Suggested Anti-DENND1B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate; DENND1B antibody - middle region (ARP52771_P050) in Human 293T cells using Western Blot
Formalin fixed, paraffin embedded human lymph tissue stained with DENND1B Antibody (N-term) Cat.-No AP51240PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of DENND1B Antibody (N-term) Cat.-No AP51240PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the DENND1B antibody detected the DENND1B protein (arrow).
Flow cytometric analysis of NCI-H460 cells using DENND1B Antibody (N-term) Cat.-No AP51240PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308414 diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. TA308414 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DENND1B(TA308414) antibody at 1:200 dilution.
WB Suggested Anti-DENND1B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Muscle
WB Suggested Anti-DENND1B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308414 | DENND1B antibody |
||||||
| Rabbit Polyclonal antibody to DENND1B (DENN/MADD domain containing 1B) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| AP51240PU-N | DENND1B (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760835 | DENND1B (full length, N-term HIS tag, transcript variant 2) |
||||
| Purified recombinant protein of Human DENN/MADD domain containing 1B (DENND1B), transcript variant 2, full length, with N-terminal HIS tag, expressed in E. coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY408118 | DENND1B overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR216682L2V | Lenti ORF particles, Dennd1b (GFP-tagged) - Mouse DENN/MADD domain containing 1B (Dennd1b), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,560.00
|
|
| OriGene Technologies, Inc. | ||
| MR216682L1V | Lenti ORF particles, Dennd1b (Myc-DDK-tagged) - Mouse DENN/MADD domain containing 1B (Dennd1b), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,400.00
|
|
| OriGene Technologies, Inc. | ||