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Desmin antibody

Principal name

Desmin antibody

Alternative names for Desmin antibody

DES

SwissProt ID

O62654 (Bovin), P02540 (Pig), P02541 (Mesau), P02542 (Chick), P17661 (Human), P23239 (Xenla), P31001 (Mouse), P48675 (Rat), Q5XFN2 (Canfa)

Gene ID

1674 (DES), 64362 (Des), 13346

Ncbi ID

NP_001918.3, NP_001918, 9606, 9031, NM_022531, NM_010043

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit), Chk (Chicken), African clawed frog, Mam (Mammalia), Hst (Hamster), Ze (Zebrafish), Gt (Goat), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Desmin antibody

Desmins belongs to the intermediate filament family, and are class III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z line structures. Defects in Desmin are the cause of desmin related cardio skeletal myopathy (CSM) also known as desmin related myopathy (DRM). CSM is characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by intracytoplasmic accumulation of desmin reactive deposits in cardiac and skeletal muscle cells. A desmin related myopathy can have a distal onset, it is then known as hereditary distal myopathy (HDM). Defects in Desmin are also the cause of dilated cardiomyopathy type 1I (CMD1I). CMD1I is an autosomal form of dilated cardiomyopathy characterized by ventricular dilatation and impaired systolic function. Antidesmin antibodies are useful in identification of tumours of myogenic origin.

General readings

Chen F, Chang R, Trivedi M, Capetanaki Y, Cryns VL.
Caspase proteolysis of desmin produces a dominant-negative inhibitor of intermediate filaments and promotes apoptosis.
J Biol Chem. 2003 Feb 28;278(9):6848-53.
PMID: 12477713

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Desmin (Phospho-Ser60) Antibody. The picture on the right is treated with the synthesized peptide.

AM06010PU-N Desmin Antibody staining of Formalin-Fixed Paraffin-Embedded Human Skeletal muscle at 1/1000-1/2000 followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AM06010PU-N

Immunohistochemistry on Rat cardiac muscle

AM20619PU-N

Immunohistochemistry (IHC) analyzes of Desmin antibody (Cat.-No.: AP06608PU-N) in paraffin-embedded Human skeletal muscle tissue.

AP06608PU-N

Western blot analysis of Desmin antibody (Cat.-No.: AP06608PU-N) in extracts from PC12 cells.

AP06608PU-N

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AP12384PU-N

Western blot analysis of anti-Desmin Antibody (Cat.#AP12384PU-N) in NCI-H460 cell line lysates (35ug/lane).Desmin (arrow) was detected using the purified Pab (1:60 dilution).

AP12384PU-N

Desmin antibody staining of Human skeletal muscle

Western blot analysis of Desmin antibody (AP20376PU-N) in extracts from HeLa cells.

AP20376PU-N

Human Jurkat; WB Suggested Anti-DES Antibody Titration: 5.0ug/ml. Positive Control: Jurkat cell lysate; DES antibody - N-terminal region (ARP41482_T100) in Human Jurkat cells using Western Blot

ARP41482_T100

Hum. Fetal Heart; Host:Rabbit. Target Name:DES. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; DES antibody - middle region (ARP41483_P050) in Hum. Fetal Heart cells using Western Blot

ARP41483_P050

Hum. Fetal Lung; Host:Rabbit. Target Name:DES. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; DES antibody - middle region (ARP41483_P050) in Hum. Fetal Lung cells using Western Blot

ARP41483_P050

Human Muscle; WB Suggested Anti-DES Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; DES antibody - middle region (ARP41483_P050) in Human Muscle cells using Western Blot

ARP41483_P050

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Desmin (Ab-60) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa/HepG2 cells, using Desmin (Ab-60) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry on frozen sections of swine stomach showing staining of muscle tissues

BM5500P

Immunohistochemistry on frozen sections of swine colon showing staining of muscle tissues

BM5500P

Immunofluorescence staining of a 7 days old Zebrafish embryo.

BM6010P

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Desmin Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from K562 cells, using Desmin Antibody. The lane on the right is treated with the synthesized peptide.

Formalin fixed paraffin embedded human leiomyoma stained with Desmin antibody. DP011

Detection limit for recombinant GST tagged DES is approximately 0.1ng/ml as a capture antibody.

H00001674-M03

DES polyclonal antibody ( Cat # PAB11453 ) ( 0.2 µg/ml ) staining of human skeletal muscle lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.

PAB11453

Immunohistochemical staining of DES on formalin fixed, paraffin embedded human leiomyosarcomas with DES polyclonal antibody.

PAB11919

IHC : Human heart tissue stained with DES polyclonal antibody ( Cat # PAB12677 ) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

PAB12677

Western blot analysis of DES polyclonal antibody ( Cat # PAB1953 ) in NCI-H460 cell line lysates ( 35 µg/lane ) .DES ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60dilution ) .

PAB1953

Human skeletal muscle stained with anti-Desmin antibody

Western blot analysis of human skeletal muscle with Desmin antibody

Immunohistochemical analysis of paraffin-embedded human smooth musde sarcoma, showing cytoplasmic localization using Desmin antibody Cat.-No AM06142PU-N with DAB staining.

AM06142PU-N

Western blot analysis using Desmin antibody Cat.-No AM06142PU-N against HEK293T cells transfected with the pCMV6-ENTRY control (Lane 1) and pCMV6-ENTRY Desmin cDNA (Lane 2).

AM06142PU-N

Immunofluorescence staining of a 9 days old Zebrafish embryo.

AP33200SU-N

Paraffin Section stained anti-Desmin Antibody Cat.-No AM33200PU (Clone BS21).

Immunohistochemical analysis of Desmin staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Desmin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of Desmin expression in Sol8 (A); HeLa (B); SJRH30 (C); PC12 (D) whole cell lysates.

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502328)

TA502328

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502328)

TA502328

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502328)

TA502328

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502328)

TA502328

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502328)

TA502328

TA502328

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DES (RC205685, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DES.

TA502328

HEK293T cells transfected with either RC205685 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DES antibody(TA502328), and then analyzed by flow cytometry.

TA502328

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-DES mouse monoclonal antibody. (UM500107, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DES mouse monoclonal antibody. (UM500107, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

TP720562

TP760253

Immunohistochemistry staining of Paraffin Muscle tissue by Desmin antibody (dilution: 1:25 - 1:100; visualization of staining: Cytoplasmic)

TA327670

TA305636 (0.5 ug/ml) staining of Pig (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA305636

TA305636 (3.8 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

TA305636

Western blot analysis of extracts from K562 cells, using Desmin antibody.The lane on the right is treated with the synthesized peptide.

TA311932

Western blot analysis of extracts from 293 cells (Lane 2), using Desmin Antibody. The lane on the left is treated with synthesized peptide.

TA311932

Immunohistochemical analysis of paraffin-embedded human muscle tissue using Desmin antibody.The picture on the right is treated with the synthesized peptide.

TA311932

WB Suggested Anti-DES Antibody Titration: 5.0 ug/ml; Positive Control: Jurkat cell lysate

TA346083

WB Suggested Anti-DES Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle

TA346084

Host: Rabbit; Target Name: DES; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml

TA346084

Host: Rabbit; Target Name: DES; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0 ug/ml

TA346084

Western blot analysis on HeLa cell lysate using Desmin Antibody

TA347369

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
UM500107	Desmin antibody
	DES mouse monoclonal antibody,clone UMAB166
	Mouse	IgG1	UMAB166	Purified	Hu	P	0.1 ml / €468.00
							OriGene Technologies, Inc.
+1 additional image	Highly validated antibody!
TA502328	Desmin antibody
	DES (Desmin ) mouse monoclonal antibody, clone OTI4G1 (formerly 4G1)
	Mouse	IgG1	OTI4G1	Purified	Hu	F, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+7 additional images	Highly validated antibody!
TA305636	Desmin (C-term) antibody
	Goat Anti-Desmin Antibody
	Goat			Aff - Purified	Bov, Can, Hu, Ms, Por, Rt	P, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
+1 additional image
TA311932	Desmin antibody
	Rabbit polyclonal anti-Desmin antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA327670	Desmin antibody
	Mouse Monoclonal Desmin Antibody
	Mouse	IgG1	D33	Purified	Hu	P	1 ml / €565.00
							OriGene Technologies, Inc.
TA347369	Desmin antibody
	Rabbit Polyclonal Anti-Desmin Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 mg / €335.00
							OriGene Technologies, Inc.
AM06010PU-N	Desmin antibody
	Mouse	IgG1	10H7D2	Purified	Hu	C, E, P, WB	50 µl / €425.00
							OriGene Technologies GmbH
AM06142PU-N	Desmin antibody
	Mouse	IgG1	10H7D2	Purified	Hu	E, P, WB	0.1 ml / €340.00
							OriGene Technologies GmbH
+1 additional image
AM20619PU-N	Desmin antibody
	Mouse	IgG1	DES-82	Purified	Hu, Ms, Rt	P, WB	0.1 mg / €390.00
							OriGene Technologies GmbH
AM32294SU-N	Desmin antibody
	Mouse	IgG1	D33	Supernatant	Hu	C, ICC/IF, P, WB	1 ml / €260.00
							OriGene Technologies GmbH
AM33200PU-N	Desmin antibody
	Mouse	IgG1	BS21	Purified	Hu	P	1 ml / €950.00
							OriGene Technologies GmbH
AM33200PU-S	Desmin antibody
	Mouse	IgG1	BS21	Purified	Hu	P	0.1 ml / €390.00
							OriGene Technologies GmbH
AP06608PU-N	Desmin antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image
AP12384PU-N	Desmin antibody
	Rabbit	Ig		Aff - Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image
AP20376PU-N	Desmin antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP720562	Desmin
	Recombinant protein of human desmin (DES)
	Human	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	10 µg / €299.00
				OriGene Technologies, Inc.
TP760253	Desmin (full length, N-term HIS tag)
	Recombinant protein of human desmin (DES), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
	Human	> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
				OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR207502L2V	Lenti ORF particles, Des (GFP-tagged) - Mouse desmin (Des), 200 uL, >10^7 TU/mL
		200 µl / €1,140.00
		OriGene Technologies, Inc.

MR207502L1V	Lenti ORF particles, Des (Myc-DDK-tagged) - Mouse desmin (Des), 200 uL, >10^7 TU/mL
		200 µl / €1,020.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR213978L2V	Lenti ORF particles, Des (GFP-tagged ORF) - Rat desmin (Des), 200 uL, >10^7 TU/mL
		200 µl / €1,150.00
		OriGene Technologies, Inc.

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