DFNA5 antibody
Principal name
DFNA5 antibody
Alternative names for DFNA5 antibody
Dfna5h, ICERE1, ICERE-1, Non-syndromic hearing impairment protein 5, Inversely correlated with estrogen receptor expression 1
Ncbi ID
9606, NP_001120925, NP_061239.1, NP_001120925.1, NP_004394, NM_018769, NM_004403, NM_001127454, NM_001127453
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of DFNA5 antibody
- Kit Component:
- - KN304518G1, Dfna5 gRNA vector 1 in pCas-Guide vector
- KN304518G2, Dfna5 gRNA vector 2 in pCas-Guide vector
- KN304518D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of DFNA5 expression in transfected 293T cell line (H00001687-T02) by DFNA5 MaxPab polyclonal antibody.Lane 1: DFNA5 transfected lysate(54.56 KDa).Lane 2: Non-transfected lysate.
Human kidney; DFNA5 antibody - C-terminal region (ARP46106_T100) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-DFNA5 Antibody Titration: 1.25 ug/ml. Positive Control: HepG2 cell lysate. DFNA5 is supported by BioGPS gene expression data to be expressed in HepG2.; DFNA5 antibody - C-terminal region (ARP46106_T100) in Human HepG2 cells using Western Blot
Western blot analysis of DFNA5 Antibody (N-term) (AP17278PU-N) in Hela cell line lysates (35 µg/lane). DFNA5 (arrow) was detected using the purified Pab.
Western blot analysis of DFNA5 expression in HeLa (A) whole cell lysates.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-DFNA5 rabbit polyclonal antibody. (TA590634)
Immunohistochemistry: Dfna5 deafness Antibody - Most of the normal cells showed strong cytoplasmic positivity. Glial cells displayed moderate cytoplasmic staining in a fraction of cells. A majority of the malignant cells displayed moderate cytoplasmic immunoreactivity. Several cases of lymphomas, melanomas, thyroid, colorectal, ovarian, endometrial cancers and cholangiocarcinomas showed strong positivity. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-DFNA5 rabbit polyclonal antibody. (TA590634)
Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFNA5 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFNA5 (RC210515, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFNA5.
ELISA: Dfna5 deafness Antibody - Affinity Purified
WB Suggested Anti-DFNA5 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate.DFNA5 is supported by BioGPS gene expression data to be expressed in HepG2
Human kidney
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP17278PU-N | DFNA5 (N-term) antibody |
||||||
 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
|||
| OriGene Technologies GmbH | |||||||
| AP55114SU-N | DFNA5 antibody |
||||||
 |
Rabbit | Serum | Ms | C, E, WB |
0.2 ml /
€430.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310515 | DFNA5 (transcript variant 1) |
||||
| Recombinant protein of human deafness, autosomal dominant 5 (DFNA5), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218826L2V | Lenti ORF particles, Dfna5 (GFP-tagged) - Mouse deafness, autosomal dominant 5 (human) (Dfna5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,210.00
|
|
| OriGene Technologies, Inc. | ||
| MR218826L1V | Lenti ORF particles, Dfna5 (Myc-DDK-tagged) - Mouse deafness, autosomal dominant 5 (human) (Dfna5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,080.00
|
|
| OriGene Technologies, Inc. | ||