DGCR8 antibody
Principal name
DGCR8 antibody
Alternative names for DGCR8 antibody
C22orf12, DGCRK6, LP4941, DiGeorge syndrome critical region 8, Microprocessor complex subunit DGCR8
Ncbi ID
9606, NP_073557, NP_001177255.1, NM_022720, NP_201581.2, NM_033324, NM_001190326
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of DGCR8 antibody
DGCR8 (UniProtKB/Swiss-Prot entry Q8WYQ5) is a component of the microprocessor complex that acts as a R- and heme-binding protein and is involved in the initial step of microR (miR) biogenesis. This microprocessor complex that is required to process primary miR transcripts (pri-miRs) to release precursor miR (pre-miR) in the nucleus. Within the microprocessor complex, DGCR8 functions as a molecular anchor necessary for the recognition of pri-miR at dsR-ssR junction and directs DROSHA to cleave 11 bp away form the junction. This releases hairpin-shaped pre-miRs that are subsequently cut by the cytoplasmic DICER to generate mature miRs. The heme-bound DGCR8 dimer binds pri-miRs as a cooperative trimer (of dimers) and is active in triggering pri-miR cleavage, whereas the heme-free DGCR8 monomer binds pri-miRs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miR are required for its binding. DGCR8 is involved in the silencing of embryonic stem cells self-renewal.
General readings
Shiohama,A., (2007) Exp. Cell Res. 313 (20), 4196-4207
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA310111 | DGCR8 antibody |
||||||
| Rabbit anti-DGCR8 polyclonal antibody | |||||||
 |
Rabbit | Purified | Hu, Rt | WB |
0.1 ml /
€220.00
|
||
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN219451 | DGCR8 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN219451BN | DGCR8 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN219451LP | DGCR8 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN219451RB | DGCR8 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP319451 | DGCR8 |
||||
| Purified recombinant protein of Homo sapiens DiGeorge syndrome critical region gene 8 (DGCR8) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402934 | DGCR8 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210572L2V | Lenti ORF particles, Dgcr8 (GFP-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,570.00
|
|
| OriGene Technologies, Inc. | ||
| MR210572L1V | Lenti ORF particles, Dgcr8 (Myc-DDK-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,410.00
|
|
| OriGene Technologies, Inc. | ||
