DIAPH1 antibody
Principal name
DIAPH1 antibody
Alternative names for DIAPH1 antibody
DIAP1, Diaphanous homolog 1, Diaphanous-1, DRF1, Diaphanous-related formin-1
Ncbi ID
9606, NP_001073280.1, NM_001079812, NP_001073280, NP_005210, NP_031884.1, NM_005219
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of DIAPH1 antibody
- Kit Component:
- - KN213517G1, DIAPH1 gRNA vector 1 in pCas-Guide vector
- KN213517G2, DIAPH1 gRNA vector 2 in pCas-Guide vector
- KN213517D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged DIAPH1 is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of DIAPH1 expression in transfected 293T cell line (H00001729-T01) by DIAPH1 MaxPab polyclonal antibody.Lane 1: DIAPH1 transfected lysate(44.44 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of DIAPH1 expression in transfected 293T cell line (H00001729-T02) by DIAPH1 MaxPab polyclonal antibody.Lane 1: DIAPH1 transfected lysate(44.44 KDa).Lane 2: Non-transfected lysate.
Sample (30 ug of whole cell lysate). A: H1299. B: Raji. 7.5% SDS PAGE. TA308571 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded gastric tissue, using DIAPH1(TA308571) antibody at 1:100 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DIAPH1(TA308571) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
(0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts of various cell lines, using DIAPH1 antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308571 | DIAPH1 antibody |
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| Rabbit Polyclonal antibody to DIAPH1 (diaphanous homolog 1 (Drosophila)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA311325 | DIAPH1 antibody |
||||||
| Goat Anti-DIAPH1 Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA332904 | DIAPH1 antibody |
||||||
| Rabbit anti-DIAPH1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP313517 | DIAPH1 (transcript variant 2) |
||||
| Recombinant protein of human diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
| TP316318 | DIAPH1 (transcript variant 1) |
||||
| Recombinant protein of human diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY421547 | DIAPH1 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |