DICER1 antibody
Principal name
DICER1 antibody
Alternative names for DICER1 antibody
Endoribonuclease Dicer, DCR1, HERNA, KIAA0928, Helicase MOI, Helicase with RNase motif
Gene ID
23405 (DICER1)
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)
Background of DICER1 antibody
RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified.
Western blot analysis of 15 µg of mouse ovary tissue lysate with Dicer1 polyclonal antibody at 1 : 1000 dilution.
Western blot analysis of DICER1 expression in Jurkat cell lysate ( Lane 1 & 2 ) and mouse small intestine tissue lysate ( Lane 3 ) .
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DICER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801631)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DICER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801631)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DICER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801633)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DICER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801633)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DICER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801636)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DICER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801644)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DICER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA801644)
Western blot analysis of HT29 cell lysate (35ug) by using anti-DICER1 monoclonal antibody. Dilution: 1:500
Immunoblot versus adult rat brain membranes (RBM) and extracts of embryonic stem (ES) cells from wild-type (WT)and Dicer knockout (KO) mice probed with N167/7 (left) and N52A/42 (right, loading control) TC supe. Mouse ES cell samples courtesy of Chryssa Kanellopoulou and David Livingston, Harvard.
Immunoblot versus adult rat brain membranes (RBM) and extracts of embryonic stem (ES) cells from wild-type (WT)and Dicer knockout (KO) mice probed with N167/7 (left) and N52A/42 (right, loading control) TC supe. Mouse ES cell samples courtesy of Chryssa Kanellopoulou and David Livingston, Harvard.
Immunohistochemistry: Dicer Antibody - IHC staining of DICER in mouse prostate.
Immunohistochemical staining of formalin-fixed paraffin-embedded brain showing cytoplasmic staining with anti-Dicer1 antibody diluted at 1:100.
Western Blot: Dicer Antibody [TA336597] - Mouse ovary tissue lysate probed with Dicer antibody at 1:1000 dilution.
ELISA: Dicer Antibody [TA336964] - ELISA assay of HeLa cell lysate transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using Dicer antibody followed by an anti rabbit IgG-HRP antibody.
Immunohistochemistry-Paraffin: Dicer Antibody [TA336964] - IHC analysis of human testis using Dicer antibody at 1:200.
Flow Cytometry: Dicer Antibody [TA336964] - Intracellular flow cytometry analysis of HeLa cells transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using this antibody. Purple represents isotype control staining.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309626 | DICER1 antibody |
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| Rabbit Polyclonal Dicer Antibody | |||||||
 |
Rabbit | Purified | Hu, Ms | C, ICC/IF |
50 µl /
€395.00
|
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| OriGene Technologies, Inc. | |||||||
| TA310112 | DICER1 antibody |
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| Rabbit anti-Dicer1 polyclonal antibody | |||||||
 |
Rabbit | Purified | Hu, Ms | P |
0.1 ml /
€220.00
|
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| OriGene Technologies, Inc. | |||||||
| TA326471 | DICER1 (1638-1899) antibody |
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| Mouse Monoclonal Anti-Dicer Antibody | |||||||
 |
Mouse | IgG1 | S167-7 | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€410.00
|
| OriGene Technologies, Inc. | |||||||
| TA336597 | DICER1 antibody |
||||||
| Rabbit Polyclonal Dicer Antibody | |||||||
![Western Blot: Dicer Antibody [TA336597] - Mouse ovary tissue lysate probed with Dicer antibody at 1:1000 dilution.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB200-591-1-W.jpg) |
Rabbit | Purified | Hu, Ms | C, P, WB |
0.1 mg /
€450.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA336964 | DICER1 antibody |
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| Rabbit Polyclonal Dicer Antibody | |||||||
![ELISA: Dicer Antibody [TA336964] - ELISA assay of HeLa cell lysate transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using Dicer antibody followed by an anti rabbit IgG-HRP antibody.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-23891-1-E.jpg) |
Rabbit | Purified | Hu | E, F, P |
0.1 ml /
€395.00
|
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA351139 | DICER1 antibody |
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| Rabbit Polyclonal Anti-DICER1 Antibody | |||||||
|
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA801631 | DICER1 antibody |
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| DICER1 mouse monoclonal antibody, clone OTI1E11 (formerly 1E11) | |||||||
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Mouse | IgG2a | OTI1E11 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA801633 | DICER1 antibody |
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| DICER1 mouse monoclonal antibody, clone OTI1D9 (formerly 1D9) | |||||||
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Mouse | IgG1 | OTI1D9 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA801636 | DICER1 antibody |
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| DICER1 mouse monoclonal antibody, clone OTI2B1 (formerly 2B1) | |||||||
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Mouse | IgG2a | OTI2B1 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA801644 | DICER1 antibody |
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| DICER1 mouse monoclonal antibody, clone OTI1G8 (formerly 1G8) | |||||||
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Mouse | IgG2b | OTI1G8 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| 73-196 | DICER1 antibody |
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 |
Mouse | IgG1 | N167/7 | Supernatant | Hu, Ms, Rt | ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
|
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| 75-196 | DICER1 antibody |
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 |
Mouse | IgG1 | N167/7 | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
|
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN211804 | DICER1 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN211804BN | DICER1 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN211804LP | DICER1 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN211804RB | DICER1 - human gene knockout kit via CRISPR |
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|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311804 | DICER1 (transcript variant 2) |
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| Purified recombinant protein of Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
| TP319214 | DICER1 (transcript variant 1) |
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| Recombinant protein of human dicer 1, ribonuclease type III (DICER1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY406165 | DICER1 overexpression lysate |
|
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0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY410795 | DICER1 overexpression lysate |
|
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0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |