Dickkopf-1 antibody
Principal name
Dickkopf-1 antibody
Alternative names for Dickkopf-1 antibody
DKK1, DKK-1, Dickkopf-related protein 1, SK
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of Dickkopf-1 antibody
DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/βcatenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/βcatenin signaling cascade. It has been suggested that by inhibiting Wnt/β-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.
General readings
Fedi P, Bafico A, Nieto Soria A, Burgess WH, Miki T, Bottaro DP, Kraus MH, Aaronson SA. Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling. J Biol Chem. 1999 Jul 2;274(27):19465-72.
DKK1 monoclonal antibody (M01), clone 4D4. Western Blot analysis of DKK1 expression in Jurkat ( Cat # L017V1 ).
DKK1 monoclonal antibody (M01), clone 4D4 Western Blot analysis of DKK1 expression in HepG2 ( Cat # L019V1 ).
DKK1 monoclonal antibody (M02), clone 1F5. Western Blot analysis of DKK1 expression in Jurkat ( Cat # L017V1 ).
DKK1 monoclonal antibody (M02), clone 1F5 Western Blot analysis of DKK1 expression in HeLa ( Cat # L013V1 ).
DKK1 monoclonal antibody (M06), clone 4C10. Western Blot analysis of DKK1 expression in Raw 264.7 ( Cat # L024V1 ).
DKK1 monoclonal antibody (M06), clone 4C10 Western Blot analysis of DKK1 expression in HeLa ( Cat # L013V1 ).
Immunoperoxidase of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
DKK1 monoclonal antibody (M08), clone 2B12 Western Blot analysis of DKK1 expression in HeLa ( Cat # L013V1 ).
DKK1 monoclonal antibody (M10), clone 1A3. Western Blot analysis of DKK1 expression in human lung cancer.
Detection limit for recombinant GST tagged DKK1 is approximately 3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
DKK1 monoclonal antibody (M11), clone 2A5 Western Blot analysis of DKK1 expression in U-2 OS ( Cat # L022V1 ).
Placenta: Formalin-Fixed Paraffin-Embedded (FFPE)
DKK1 polyclonal antibody ( Cat # PAB6672 ) ( 1 µg/ml ) staining of human bone marrow lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
DKK1 monoclonal antibody (M04), clone 3D9 Western Blot analysis of DKK1 expression in HeLa ( Cat # L013V1 ).
DKK1 monoclonal antibody (M05), clone 3G3 Western Blot analysis of DKK1 expression in HeLa ( Cat # L013V1 ).
DKK1 monoclonal antibody (M12), clone 3C3. Western Blot analysis of DKK1 expression in HeLa ( Cat # L013V1 ).
Human; . Lanes: Lane 1: 30ug human PLC/PRF5 cell lysate. Lane 2: 30ug DKK1 PLC/PRF5 cell lysate . Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP Anti-rabbit-HRP. Secondary Antibody Dilution: 1:10,000. Gene Name: DKK1 a. Submitted by: Dr Frankie Ko Chi Fat, Lo-Kong Chan, Irene O.L. Ng, Judy Wai Ping Yam; Department of Pathology, The University of Hong Kong.; DKK1 antibody - C-terminal region (ARP48015_T100) in Human cells using Western Blot
Human HepG2; WB Suggested Anti-DKK1 Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; DKK1 antibody - C-terminal region (ARP48015_T100) in Human HepG2 cells using Western Blot
Recombinant human Dkk-1, aa 32-266, His-tagged: 15% SDS-PAGE (3 µg)
Proximity Ligation Analysis (PLA) of protein-protein interactions between DKK1 and CTNNB1 HeLa cells were stained with anti-DKK1 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of DKK1 expression in transfected 293T cell line (H00022943-T02) by DKK1 MaxPab polyclonal antibody.Lane 1: DKK1 transfected lysate(28.70 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of Dkk1 in Lysates from NIH/3T3 cells ( Lane 1 ) and rat kidney ( Lane 2 ) .
Western blot analysis of Dickkopf-1 Antibody (N-term) Cat.-No AP51269PU-N in T47D cell line lysates (35ug/lane). DKK1 (arrow) was detected using the purified Pab.
Western blot analysis using DKK1 antibody Cat.-No AM06319SU-N against HEK293 (Lane 1) and DKK1-hIgGFc transfected HEK293 cell lysate (Lane 2).
Representative Standard Curve
Representative Standard Curve
TA302464 (1µg/ml) staining of human bone marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Host: Rabbit; Target Name: DKK1; Sample Tissue: PANC1 Whole Cell lysates; Antibody Dilution: 1.0 ug/mlDKK1 is supported by BioGPS gene expression data to be expressed in PANC1
Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DKK1 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302464 | Dickkopf-1 (C-term) antibody |
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| Goat Polyclonal Antibody against DKK1 | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA341113 | Dickkopf-1 (1st Cytopl. Dom.) antibody |
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| Rabbit Polyclonal Anti-DKK1 Antibody (Cytoplasmic Domain) | |||||||
 |
Rabbit | Aff - Purified | Hu, Mky, Prim | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341114 | Dickkopf-1 antibody |
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| Rabbit Polyclonal Anti-DKK1 Antibody (Internal) | |||||||
 |
Rabbit | Purified | GP, Gt, Hu, Rb, Sh |
50 µg /
€425.00
|
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| OriGene Technologies, Inc. | |||||||
| TA341115 | Dickkopf-1 (N-term) antibody |
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| Rabbit Polyclonal Anti-DKK1 Antibody (N-Terminus) | |||||||
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Rabbit | Aff - Purified | Bear, Bov, Bt, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Prim, Rb, Rt | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341116 | Dickkopf-1 antibody |
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| Rabbit Polyclonal Anti-DKK1 Antibody (N-Terminus) | |||||||
 |
Rabbit | Purified | Bov, Eq, Hu, Mky, Por, Rb |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AM06319SU-N | Dickkopf-1 antibody |
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 |
Mouse | IgG2b | 2H2 | Ascites | Hu | WB |
0.1 ml /
€340.00
|
| OriGene Technologies GmbH | |||||||
| AP07519PU-N | Dickkopf-1 (C-term) antibody |
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 |
Goat | Aff - Purified | Bov, Can, Elephant, Eq, Gt, Hu, Mky, Ms, Platypus, Por | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| AP51269PU-N | Dickkopf-1 (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP710289 | Dickkopf-1 (full length, C-term DDK tag) |
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| Purified recombinant protein of Human dickkopf homolog 1 (Xenopus laevis) (DKK1), full length, with C-terminal DDK tag, expressed in sf9, 20ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
| TP723065 | Dickkopf-1 |
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| Purified recombinant protein of Human dickkopf homolog 1 (Xenopus laevis) (DKK1). | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€230.00
|
|
| OriGene Technologies, Inc. | |||||
ELISA Kits
| Catalog No. | ||
|---|---|---|
| EA100582 | Mouse DKK1 ELISA Kit |
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1 kit /
€500.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203629L2V | Lenti ORF particles, Dkk1 (GFP-tagged) - Mouse dickkopf homolog 1 (Xenopus laevis) (Dkk1), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
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| OriGene Technologies, Inc. | ||
| MR203629L1V | Lenti ORF particles, Dkk1 (Myc-DDK-tagged) - Mouse dickkopf homolog 1 (Xenopus laevis) (Dkk1), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
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| OriGene Technologies, Inc. | ||