Dihydrolipoyl dehydrogenase antibody
Principal name
Dihydrolipoyl dehydrogenase antibody
Alternative names for Dihydrolipoyl dehydrogenase antibody
DLD, GCSL, LAD, PHE3, EC 1.8.1.4, Dihydrolipoamide dehydrogenase, Glycine cleavage system L protein
Ncbi ID
NP_000099.2, 9823, NP_000099, NP_031887.2, NM_007861, NM_000108
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F)
Background of Dihydrolipoyl dehydrogenase antibody
DLD is the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Giannopoulou E., et al. (2009) Anticancer Res. 29(12):5077-82.
Daee DL., et al. (2009) Proc Natl Acad Sci U S A. 107(1):157-62.
Immunoperoxidase of monoclonal antibody to DLD on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]
DLD monoclonal antibody (M02), clone 3C1 Western Blot analysis of DLD expression in Hela ( Cat # L013V1 ).
Detection limit for recombinant GST tagged DLD is approximately 1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to DLD on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged DLD is approximately 1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to DLD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged DLD is approximately 1ng/ml as a capture antibody.
Immunohistochemistry of formalin-fixed,paraffin-embedded human heart tissue after heat-induced antigen retrieval using Cat.-No. AP07395PU-N,concentration 5 ug/ml.
Human Pineal Tissue; Rabbit Anti-DLD Antibody . Catalog Number: ARP58455_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-DLD Antibody (ARP58455_P050) ) in Human Pineal Tissue using Immunohistochemistry
Human Hela; Host: Rabbit. Target Name: EGFL8 . Sample Tissue: Hela . Antibody Dilution: 1.0ug/ml. DLD is supported by BioGPS gene expression data to be expressed in HeLa.; DLD antibody - middle region (ARP58455_P050) in Human Hela cells using Western Blot
Human MCF7; Host: Rabbit. Target Name: NOP56 . Sample Tissue: MCF7 . Antibody Dilution: 1.0ug/ml. DLD is supported by BioGPS gene expression data to be expressed in MCF7.; DLD antibody - middle region (ARP58455_P050) in Human MCF7 cells using Western Blot
Rat kidney; DLD antibody - middle region (ARP58455_P050) validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.; DLD antibody - middle region (ARP58455_P050) in Rat kidney cells using Western Blot
Human Jurkat; WB Suggested Anti-DLD Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. DLD is supported by BioGPS gene expression data to be expressed in Jurkat.; DLD antibody - middle region (ARP58455_P050) in Human Jurkat cells using Western Blot
Hum. Adult Placenta; Host: Rabbit. Target Name: SERPINA3 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; DLD antibody - middle region (ARP58455_P050) in Hum. Adult Placenta cells using Western Blot
Recombinant human DLD, 36-509 aa, His-tagged: 15% SDS-PAGE (3 µg)
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of DLD expression in Hela cell lysate.
Detection limit for recombinant GST tagged DLD is approximately 1ng/ml as a capture antibody.
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged DLD is approximately 1ng/ml as a capture antibody.
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged DLD is approximately 1ng/ml as a capture antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503387)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503387)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503387)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503387)
Anti-DLD mouse monoclonal antibody (TA503387) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD(RC200639).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (RC200639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DLD monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
HEK293T cells transfected with either RC200639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DLD antibody(TA503387), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DLD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (RC200639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD.
HEK293T cells transfected with either RC200639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DLD antibody(TA503388), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503390)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503390)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503390)
Anti-DLD mouse monoclonal antibody (TA503390) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD(RC200639).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (RC200639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD.
HEK293T cells transfected with either RC200639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DLD antibody(TA503390), and then analyzed by flow cytometry.
Anti-DLD mouse monoclonal antibody (TA503391) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD(RC200639).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (RC200639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DLD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC200639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DLD antibody(TA503391), and then analyzed by flow cytometry.
Anti-DLD mouse monoclonal antibody (TA503421) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD(RC200639).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (RC200639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD.
HEK293T cells transfected with either RC200639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DLD antibody(TA503421), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503422)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503422)
Anti-DLD mouse monoclonal antibody (TA503422) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD(RC200639).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (RC200639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD.
HEK293T cells transfected with either RC200639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DLD antibody(TA503422), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503423)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503423)
Anti-DLD mouse monoclonal antibody (TA503423) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD(RC200639).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (RC200639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD.
HEK293T cells transfected with either RC200639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DLD antibody(TA503423), and then analyzed by flow cytometry.
Western blot analysis of extracts of various cell lines, using DLD antibody.
Immunohistochemistry of paraffin-embedded human breast cancer using DLD antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using DLD antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded human stomach using DLD antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded human stomach using DLD antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded mouse lung using DLD antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of U20S cell using DLD antibody. Blue: DAPI for nuclear staining.
DLD antibody - middle region validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.
2Hum. Adult Placenta; Host: Rabbit; Target Name: SERPINA3; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-DLD Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateDLD is supported by BioGPS gene expression data to be expressed in Jurkat
4Human MCF7; Host: Rabbit; Target Name: NOP56; Sample Tissue: MCF7; Antibody Dilution: 1.0 ug/mlDLD is supported by BioGPS gene expression data to be expressed in MCF7
5Human Hela; Host: Rabbit; Target Name: EGFL8; Sample Tissue: Hela; Antibody Dilution: 1.0 ug/mlDLD is supported by BioGPS gene expression data to be expressed in HeLa
Rabbit Anti-DLD Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue; Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes; Primary Antibody Concentration: 1:100; Secondary Antibody: Donkey anti-Rabbit-Cy3; Seconda
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (DLD Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (DLD Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (6)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332669 | Dihydrolipoyl dehydrogenase antibody |
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| Rabbit anti-DLD Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody! |
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| TA503387 | Dihydrolipoyl dehydrogenase antibody |
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| DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone OTI6D5 (formerly 6D5) | |||||||
 |
Mouse | IgG1 | OTI6D5 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA503390 | Dihydrolipoyl dehydrogenase antibody |
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| DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone OTI4E11 (formerly 4E11) | |||||||
 |
Mouse | IgG1 | OTI4E11 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA503391 | Dihydrolipoyl dehydrogenase antibody |
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| DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone OTI5G7 (formerly 5G7) | |||||||
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Mouse | IgG1 | OTI5G7 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA503422 | Dihydrolipoyl dehydrogenase antibody |
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| DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone OTI4A10 (formerly 4A10) | |||||||
 |
Mouse | IgG1 | OTI4A10 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA503423 | Dihydrolipoyl dehydrogenase antibody |
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| DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone OTI4D5 (formerly 4D5) | |||||||
 |
Mouse | IgG1 | OTI4D5 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA349885 | Dihydrolipoyl dehydrogenase antibody |
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| Rabbit Polyclonal Anti-DLD Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA503388 | Dihydrolipoyl dehydrogenase antibody |
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| DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone OTI8A10 (formerly 8A10) | |||||||
 |
Mouse | IgG1 | OTI8A10 | Purified | Can, Hu, Mky, Ms, Rt | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA503421 | Dihydrolipoyl dehydrogenase antibody |
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| DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone OTI6G6 (formerly 6G6) | |||||||
 |
Mouse | IgG1 | OTI6G6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AM31776PU-N | Dihydrolipoyl dehydrogenase antibody |
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 |
Mouse | IgG3 | 3C1 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AM31777PU-N | Dihydrolipoyl dehydrogenase antibody |
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 |
Mouse | IgG3 | 1G11 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AM31778PU-N | Dihydrolipoyl dehydrogenase antibody |
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 |
Mouse | IgG3 | 2D4 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP07395PU-N | Dihydrolipoyl dehydrogenase antibody |
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Rabbit | Purified | Hu, Por | E, ICC/IF, IP, P, WB |
50 µl /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| R1120 | Dihydrolipoyl dehydrogenase antibody |
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Rabbit | Serum | Por | E, WB |
2 ml /
€1,345.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300639 | Dihydrolipoyl dehydrogenase |
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| Recombinant protein of human dihydrolipoamide dehydrogenase (DLD) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208182L2V | Lenti ORF particles, Dld (GFP-tagged) - Mouse dihydrolipoamide dehydrogenase (Dld), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,200.00
|
|
| OriGene Technologies, Inc. | ||
| MR208182L1V | Lenti ORF particles, Dld (Myc-DDK-tagged) - Mouse dihydrolipoamide dehydrogenase (Dld), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,070.00
|
|
| OriGene Technologies, Inc. | ||
