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DLL3 antibody

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Principal name

DLL3 antibody

Alternative names for DLL3 antibody

Delta-like protein 3, Delta-3

SwissProt ID

O88516 (Mouse), O88671 (Rat), P54655 (Xenla), Q9NYJ7 (Human)

Gene ID

10683 (DLL3), 13389 (Dll3), 114125

Ncbi ID

NP_058637, NP_058637.1, NM_016941, NP_031892.2, NM_007866, NM_053666

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate), Por (Porcine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DLL3 antibody

DLL3(DELTA-LIKE 3) also known as DELTA, DROSOPHILA, HOMOLOG OF, is a protein which in humans is encoded by the DLL3 gene. This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutation in the mouse delta-like 3 gene (Dll3), which is homologous to the Notch-ligand delta in Drosophila, results in the mouse 'pudgy' phenotype. The human DLL3 gene was identified within a critical interval, mapped in 2 consanguineous Arab-Israeli and Pakistani SCDO1 pedigrees, of 7.8 cM at 19q13.1-q13.3 between D19S570 and D19S908 (Bulman et al., 2000). Dunwoodie et al. (1997) presented results suggesting that mouse Dll3 may complement the function of other delta homologs during early pattern formation in the mouse embryo. In humans, the fact that mutations in genes required for oscillation, such as DLL3, result in abnormal segmentation of the vertebral column suggests that the segmentation clock also acts during human embryonic development. This residue is highly conserved in Delta proteins from Drosophila to humans, and the substitution of a charged polar for a nonpolar residue may disrupt the conformation of the DLL3 protein.

General readings

Yerges, L.M. et al., J. Bone Miner. Res. 24(12): 2039-2049 (2009)
Heuss, S.F. et al., Proc. Natl. Acad. Sci. U.S.A. 105(32): 11212-11217 (2008)
Maisenbacher, M.K., et al. Hum. Genet. 116(5): 416-419 (2005)
Whittock, N.V. et al., Clin. Genet. 66(1): 67-72 (2004)
Bonafe, L. et al., Clin. Genet. 64(1): 28-35 (2003)

ARP47292_P050 - DLL3
Human HepG2; WB Suggested Anti-DLL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; DLL3 antibody - N-terminal region (ARP47292_P050) in Human HepG2 cells using Western Blot
AP51273PU-N - DLL3
Immunohistochemistry analysis in human brain tissue (Formalin-fixed, Paraffin-embedded) using DLL3 antibody Cat.-No. AP51273PU-N (C-term), followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of DLL3 antibody for IHC. Clinical relevance has not been evaluated.
AP51273PU-N - DLL3
Confocal immunofluorescent analysis with 293 cells using DLL3 antibody Cat.-No. AP51273PU-N (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
AP51273PU-N - DLL3
Western blot analysis in 293 cell line lysates (35ug/lane) using DLL3 antibody Cat.-No. AP51273PU-N (C-term). This demonstrates the DLL3 antibody detected the DLL3 protein (arrow).
AP51274PU-N - DLL3
Immunohistochemistry anaysis of human brain tissue (Formalin-fixed, Paraffin-embedded) using DLL3 antibody Cat.-No. AP51274PU-N (C-term), follwed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.
AP51274PU-N - DLL3
All lanes: Anti-DLL3 Antibody (C-term) at 1/2000 dilution. Lane 1: A375 whole cell lysate. Lane 2: Human brain lysate. Lane 3: Human liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP51274PU-N - DLL3
Confocal immunofluorescent analysis with 293 cell using DLL3 antibody Cat.-No. AP51274PU-N (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
AP51274PU-N - DLL3
Western blot analysis of DLL3 (arrow) in A375 cell line lysates (35ug/lane) using DLL3 antibody Cat.-No. AP51274PU-N.
TA337030 - DLL3
Western Blot: DLL3 Antibody [TA337030] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary ant
TA337030 - DLL3
Immunohistochemistry-Paraffin: DLL3 Antibody [TA337030] - Formalin-fixed, paraffin-embedded human brain tissue probed with an isotype control (top) and DLL3 antibody (bottom) at 5 ug/ml.
TA342025 - DLL3
Host: Rabbit; Target Name: DLL3; Sample Tissue: Hela Whole cell lysates; Antibody Dilution: 1.0 ug/ml
TA342026 - DLL3
WB Suggested Anti-DLL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate
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  2. Highly Validated (1)

15 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DLL3 (C-term) antibody
Immunohistochemistry anaysis of human brain tissue (Formalin-fixed, Paraffin-embedded) using DLL3 antibody Cat.-No. AP51274PU-N (C-term), follwed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+6 additional images

Highly validated antibody!

DLL3 antibody
  Rabbit Polyclonal DLL3 Antibody  
Western Blot: DLL3 Antibody [TA337030] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary ant Rabbit Purified Hu, Ms, Prim P, WB
0.1 mg / €395.00
  OriGene Technologies, Inc.
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

DLL3 - human gene knockout kit via CRISPR
DLL3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DLL3 - human gene knockout kit via CRISPR
DLL3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DLL3 - human gene knockout kit via CRISPR
DLL3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DLL3 - human gene knockout kit via CRISPR
DLL3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dll3 - mouse gene knockout kit via CRISPR
Dll3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dll3 - mouse gene knockout kit via CRISPR
Dll3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dll3 - mouse gene knockout kit via CRISPR
Dll3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dll3 - mouse gene knockout kit via CRISPR
Dll3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

DLL3 overexpression lysate
DLL3 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dll3 (GFP-tagged) - Mouse delta-like 3 (Drosophila) (Dll3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Dll3 (GFP-tagged) - Mouse delta-like 3 (Drosophila) (Dll3), 200 uL, >10^7 TU/mL  
200 µl / €1,310.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Dll3 (Myc-DDK-tagged) - Mouse delta-like 3 (Drosophila) (Dll3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Dll3 (Myc-DDK-tagged) - Mouse delta-like 3 (Drosophila) (Dll3), 200 uL, >10^7 TU/mL  
200 µl / €1,170.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dll3 (GFP-tagged ORF) - Rat delta-like 3 (Drosophila) (Dll3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Dll3 (GFP-tagged ORF) - Rat delta-like 3 (Drosophila) (Dll3), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Dll3 (Myc-DDK-tagged ORF) - Rat delta-like 3 (Drosophila) (Dll3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Dll3 (Myc-DDK-tagged ORF) - Rat delta-like 3 (Drosophila) (Dll3), 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.
 

15 Item(s)

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15 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (8)
  2. Lysate (1)
  3. Mouse Lenti ORF Particle (2)
  4. Primary Antibody (2)
  5. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (2)
Target
  1. DLL3 (2)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (1)
  3. Primate (Prim) (1)
Applications
  1. Paraffin Sections (P) (2)
  2. Western blot / Immunoblot (WB) (2)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Host
  1. Rabbit (2)
Images
  1. 1 image (2)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. IHC (1)
  4. Paraffin Sections (P) (1)
Species (CRISPR)
  1. Human (4)
  2. Mouse (4)
Donor Vector
  1. GFP-Puro (2)
  2. Luciferase-Puro (2)
  3. RFP-BSD (2)
  4. mBFP-Neo (2)
Manufacturer
  1. OriGene Technologies (15)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • fax: +49-5221-34606-11
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