DNA primase small subunit antibody
Principal name
DNA primase small subunit antibody
Alternative names for DNA primase small subunit antibody
PRIM1, DNA primase 49 kDa subunit
Ncbi ID
9606, NP_000937, NP_000937.1, NM_008921, NM_000946, NP_032947.1
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of DNA primase small subunit antibody
- Kit Component:
- - KN313863G1, Prim1 gRNA vector 1 in pCas-Guide vector
- KN313863G2, Prim1 gRNA vector 2 in pCas-Guide vector
- KN313863D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Arezi,B., (1999) Biochemistry 38 (39), 12899-12907
Human K562; WB Suggested Anti-PRIM1 Antibody Titration: 1.25ug/ml. Positive Control: K562 cell lysate; PRIM1 antibody - N-terminal region (ARP46013_T100) in Human K562 cells using Western Blot
Immunohistochemistry (IHC) analyzes of PRIM1 antibody (Cat.-No.: AP21164PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of PRIM1 antibody (Cat.-No.: AP21164PU-N) in extracts from Raw264.7 cells, treated with LPS.
Mouse Kidney; WB Suggested Anti-Prim1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Kidney; Prim1 antibody - C-terminal region (ARP46014_P050) in Mouse Kidney cells using Western Blot
Immunohistochemical analysis of PRIM1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PRIM1 expression in HeLa (A); Raw264.7 (B) whole cell lysates.
Western blot analysis of extracts from HepG2 cells, using PRIM1 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-PRIM1 Antibody Titration: 1.25 ug/ml; Positive Control: K562 cell lysate
WB Suggested Anti-Prim1 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Kidney
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314378 | DNA primase small subunit antibody |
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| Rabbit polyclonal anti-PRIM1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP21164PU-N | DNA primase small subunit antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY424441 | PRIM1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206605L2V | Lenti ORF particles, Prim1 (GFP-tagged) - Mouse DNA primase, p49 subunit (Prim1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206605L1V | Lenti ORF particles, Prim1 (Myc-DDK-tagged) - Mouse DNA primase, p49 subunit (Prim1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||