DNAL1 antibody
Principal name
DNAL1 antibody
Alternative names for DNAL1 antibody
Dynein light chain 1 axonemal, C14orf168
Ncbi ID
9606, NP_001188295.1, NP_001188295, NM_031427, NP_113615, NP_113615.1, NP_083097.2, NM_028821
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of DNAL1 antibody
- Kit Component:
- - KN304705G1, Dnal1 gRNA vector 1 in pCas-Guide vector
- KN304705G2, Dnal1 gRNA vector 2 in pCas-Guide vector
- KN304705D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Horváth J, Fliegauf M, et al. (2005). Am J Respir Cell Mol Biol. 33(1):41-7.Lancaster MA, et al. (2009). Curr Opin Genet Dev. 19(3):220-9.
Western blot analysis of DNAL1 in NIH/3T3 cell lysate with DNAL1 polyclonal antibody ( Cat # PAB13055 ) at ( A ) 1 and ( B ) 2 µg/ml.
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of DNAL1 in 3T3 cell lysate with DNAL1 antibody at (A) 1 and (B) 2 µg/ml.
Human HepG2; WB Suggested Anti-DNAL1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: HepG2 Whole Cell; DNAL1 antibody - N-terminal region (ARP62202_P050) in Human HepG2 cells using Western Blot
The cell lysates of HeLa, HepG2 and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DNAL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunohistochemical analysis of DNAL1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of DNAL1 expression in HEK293T (A); mouse testis (B); rat kidney (C) whole cell lysates.
Western blot analysis of DNAL1 in 3T3 cell lysate with DNAL1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry of DNAL1 in 3T3 cells with DNAL1 antibody at 2.5 ug/mL.
Western blot analysis of extracts from HepG2 cells, HeLa cells and MCF-7cells, using DNAL1 antibody.The lane on the right is treated with the synthesized peptide.
Host: Rabbit; Target Name: Dnalc1; Sample Tissue: Mouse Liver lysates; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306620 | DNAL1 antibody |
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| Rabbit Polyclonal DNAL1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA315873 | DNAL1 antibody |
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| Rabbit polyclonal anti-DNAL1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM50072PU-N | DNAL1 antibody |
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 |
Mouse | IgG2b | AT29E4 | Purified | Hu | E, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| AM50072PU-S | DNAL1 antibody |
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|
Mouse | IgG2b | AT29E4 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| AP23469PU-N | DNAL1 (C-term) antibody |
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 |
Rabbit | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302768 | DNAL1 |
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| Recombinant protein of human dynein, axonemal, light chain 1 (DNAL1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410537 | DNAL1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR216479L2V | Lenti ORF particles, Dnalc1 (GFP-tagged) - Mouse dynein, axonemal, light chain 1 (Dnalc1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR216479L1V | Lenti ORF particles, Dnalc1 (Myc-DDK-tagged) - Mouse dynein, axonemal, light chain 1 (Dnalc1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||