DNMT3A antibody
Principal name
DNMT3A antibody
Alternative names for DNMT3A antibody
DNA MTase HsaIIIA, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA
Ncbi ID
9606, NP_072046, 10090, NM_175629, NM_022552, NP_072046.2, NP_031898.1, NM_007872, NM_153743, NM_175630, NM_001003957, NM_001003958
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ChIP assay (ChIP), Enzyme Immunoassay (E), Flow Cytometry (F)
Background of DNMT3A antibody
Methylation of D at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development. Three families of mammalian D methyltransferases have been identified: DNMT1, DNMT2 and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintence methyltransferase, transferring proper methylation patterns to newly synthesized D during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of D. DNMT2 is expressed at low levels in adult somatic tissues and its ictivation affects neither de novo nor maintence D methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptiol repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of D methylation and facilitate gene silencing. Improper D methylation contributes to diseased states such as cancer. Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic D correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas.
Figure 1. Western blot analysis of DNMT3A Antibody (C-term) (Cat.# AP11035PU-N) in HepG2 cell line lysates (35ug/lane). DNMT3A (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-DNMT3A Antibody (Center D472) (Cat.#AP11050PU-N) in Jurkat cell line lysates (35ug/lane). DNMT3A(arrow) was detected using the purified Pab (1/60 dilution).
Western blot analysis of DNMT3A Antibody (Center) Cat.-No AP51302PU-N in HL-60 cell line lysates (35ug/lane).This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).
Western blot analysis of DNMT3A Antibody (C-term) Cat.-No AP51303PU-N in CEM cell line lysates (35ug/lane). This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).
Immunofluorescence of purified MaxPab antibody to DNMT3A on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of DNMT3A expression in transfected 293T cell line (H00001788-T01) by DNMT3A MaxPab polyclonal antibody.Lane1:DNMT3A transfected lysate(100.32 KDa).Lane2:Non-transfected lysate.
Immunoprecipitation of DNMT3A transfected lysate using anti-DNMT3A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DNMT3A MaxPab mouse polyclonal antibody (B01) (H00001788-B01).
Western Blot analysis of DNMT3A expression in transfected 293T cell line (H00001788-T01) by DNMT3A MaxPab polyclonal antibody.Lane 1: DNMT3A transfected lysate(101.90 KDa).Lane 2: Non-transfected lysate.
Immuno-staining of Dnmt3a. Using Dnmt3a monoclonal antibody, clone 64B1446 ( Cat # MAB0080 ) at 5 µg/ml dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.
Western blot analysis of Dnmt3a in ( A ) Dnmt3a transfected 293 cell lysate and ( B ) untransfected 293 cell lysate. Using Dnmt3a monoclonal antibody, clone 64B1446 ( Cat # MAB0080 ) at 1 µg/ml.
Nuclear extracts of HeLa cells ( 40 µg ) were analysed by Western blot using Dnmt3a polyclonal antibody ( Cat # PAB14074 ) diluted 1 : 500 in TBS-Tween containing 5% skimmed milk.
ELISA was performed using a serial dilution of Dnmt3a polyclonal antibody ( Cat # PAB14074 ).The wells were coated with the peptides used for immunisation of the rabbit.By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 86,500.
Human embryonic kidney cells ( HEK293T ) were transiently transfected with an expression vector for GAL4-tagged DNMT3A.Whole cell extracts were immunoprecipitated with 2 µg of DNMT3A polyclonal antibody ( Cat # PAB14129 ).The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody ( Lane 1 and 2 respectively ).Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A polyclonal antibody ( Cat # PAB14129 ) ( diluted 1 : 2,000 ) ( Lane 3 ).
ELISA was performed using a serial dilution of DNMT3A polyclonal antibody ( Cat # PAB14129 ), crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 500.
Human embryonic kidney cells ( HEK293T ) were transiently transfected with an expression vector for GAL4-tagged DNMT3A.Whole cell extracts were immunoprecipitated with 2 µg of DNMT3A polyclonal antibody ( Cat # PAB14130 ).The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with DNMT3A polyclonal antibody ( Cat # PAB14130 ) ( Lane 1 and 2 respectively ).Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody ( diluted 1 : 2,500 ) ( Lane 3 ).
ELISA was performed using a serial dilution of DNMT3A polyclonal antibody ( Cat # PAB14130 ), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 2,000.
Human embryonic kidney cells ( HEK293T ) were transiently transfected with an expression vector for GAL4-tagged DNMT3A.Whole cell extracts were immunoprecipitated with 2 µg of DNMT3A polyclonal antibody ( Cat # PAB14131 ).The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody ( Lane 1 and 2 respectively ).Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with DNMT3A polyclonal antibody ( diluted 1 : 2,000 ) ( Lane 3 ).
ELISA was performed using a serial dilution of DNMT3A polyclonal antibody ( Cat # PAB14131 ), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 400.
Western blot analysis of DNMT3A polyclonal antibody ( Cat # PAB2191 ) in HepG2 cell line lysates ( 35 µg/lane ) . DNMT3A ( arrow ) was detected using the purified polyclonal antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody DNMT3A polyclonal antibody ( Cat # PAB2204 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of DNMT3A polyclonal antibody ( Cat # PAB2204 ) in T-47D cell lysate. DNMT3A ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of DNMT3A polyclonal antibody ( Cat # PAB2205 ) in Jurkat cell line lysates ( 35 µg/lane ) . DNMT3A ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western blot analysis of DNMT3A polyclonal antibody ( Cat # PAB2206 ) in Jurkat cell line lysates ( 35 µg/lane ) . DNMT3A ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-Dnmt3a Pab (Cat.#AP11049PU-N) in T47-D cell lysate. Dnmt3a (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Dnmt3a antibody (N-term) (Cat.#AP11051PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-Dnmt3a Antibody (N-term R46) (Cat.#AP11051PU-N) in Jurkat cell line lysates (35ug/lane). Dnmt3a(arrow) was detected using the purified Pab (1/60 dilution).
Western blot analysis of extracts of various cell lines, using DNMT3A antibody.
Immunofluorescence analysis of U2OS cell using DNMT3A antibody. Blue: DAPI for nuclear staining.
Western blot analysis to detect overexpressed DNMT3A level in HCT-116 cells. Lane 1: 40 ug of nuclear extract from untreated HCT-116 cells. Lane 2: 40 ug of Nuclear extract from HCT-116 cells overexpressing DNMT3A.
Immunohistochemistry: Dnmt3a antibody was tested in human testis using DAB with hematoxylin counterstain.
Immunocytochemistry/Immunofluorescence: Dnmt3a antibody was tested in Ntera-2 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Predicted band size: 78 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human stomach cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human stomach cancer tissue using DNMT3A antibody at dilution 1/65, on the right is treated with the fusion protein. (Original magnification: x200)
Western blot analysis of Jurkat cell using TA322196(DNMT3A Antibody) at dilution 1/1350
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA322196(DNMT3A Antibody)(left image) at dilution 1/65, the image on the right is treated with the fusion protein.(Original magnification: x200)
Western Blot: Dnmt3a Antibody (64B814.1) [TA336383] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate (Novus, 40212) using Dnmt3a antibody at 1 ug/ml.
Western Blot: Dnmt3a Antibody (64B814.1) [TA336383] - analysis of Dnmt3a using Dnmt3a antibody at 2 ug/ml in 293 cell lysate transfected with either mouse Dnmt3a (lane 1) or mouse Dnmt3b (lane 2).
Western Blot: Dnmt3a Antibody (64B814.1) [TA336383] - WB for Dnmt3a in mouse brain cells. Image from verified customer review.
Western Blot: Dnmt3a Antibody (64B1446) [TA336580] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: Dnmt3a Antibody (64B1446) [TA336580] - Formalin-fixed, paraffin-embedded human liver stained with Dnmt3a antibody at 5 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate b
Immunocytochemistry/Immunofluorescence: Dnmt3a Antibody (64B1446) [TA336580] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with neuron-specific tubulin (green) throughout the de
Flow Cytometry: Dnmt3a Antibody (64B1446) [TA336580] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was
Western Blot: Dnmt3a Antibody [TA336891] - analysis using Dnmt3a antibody at 5 ug/ml with Dnmt3a transfected 293 cell lysate .
Western Blot: Dnmt3a Antibody [TA336977] - Analysis of Dnmt3a in Dnmt3a transfected 293 cell lysate using TA336977 at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
WB analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and WB analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3).
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody against human DNMT3A, crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:500.
WB analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and WB analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3).
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human DNMT3A, crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.
WB analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and WB analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3).
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human DNMT3A, crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:400.
C15310107-1,WB using the antibody against mouse DNMT3A diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
To determine the titer of the antibody, ELISA was performed using a serial dilution of ab against mouse DNMT3A. The wells were coated with the peptides used as antigen. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:86,500. Figure 2: WB using the ab against mouse DNMT3A at 1:500 in TBS-Tween containing 5% milk.
Host: Rabbit; Target Name: DNMT3A; Sample Tissue: Lung Tumor lysates; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA301633 | DNMT3A antibody |
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| Rabbit Polyclonal Antibody against DNMT3a | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€395.00
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA322195 | DNMT3A antibody |
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| Anti-DNMT3A Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | P |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA322196 | DNMT3A antibody |
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| Anti-DNMT3A Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA327251 | DNMT3A antibody |
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| DNMT3A Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336383 | DNMT3A antibody |
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| Mouse Monoclonal DNMT3A Antibody (64B814.1) | |||||||
![Western Blot: Dnmt3a Antibody (64B814.1) [TA336383] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate (Novus, 40212) using Dnmt3a antibody at 1 ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-56521-1-W.jpg) |
Mouse | IgG1 | 64B814.1 | Purified | Hu, Ms | ICC/IF, WB |
0.1 mg /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336580 | DNMT3A antibody |
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| Mouse Monoclonal DNMT3A Antibody (64B1446) | |||||||
![Western Blot: Dnmt3a Antibody (64B1446) [TA336580] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB120-13888-1-W.jpg) |
Mouse | IgG1 | 64B1446 | Purified | Hu, Ms | ChIP, F, ICC/IF, P, WB |
0.1 mg /
€475.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA336891 | DNMT3A antibody |
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| Rabbit Polyclonal DNMT3A Antibody | |||||||
![Western Blot: Dnmt3a Antibody [TA336891] - analysis using Dnmt3a antibody at 5 ug/ml with Dnmt3a transfected 293 cell lysate .](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-03987-1-W.jpg) |
Rabbit | Purified | Hu | WB |
0.1 mg /
€395.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA336977 | DNMT3A antibody |
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| Rabbit Polyclonal DNMT3A Antibody | |||||||
![Western Blot: Dnmt3a Antibody [TA336977] - Analysis of Dnmt3a in Dnmt3a transfected 293 cell lysate using TA336977 at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-24471-1-W.jpg) |
Rabbit | Purified | Hu, Prim | WB |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA347112 | DNMT3A antibody |
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| Rabbit Polyclonal DNMT3A Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E |
50 µg /
€395.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA347113 | DNMT3A antibody |
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| Rabbit Polyclonal DNMT3A Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E |
50 µg /
€395.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA347114 | DNMT3A antibody |
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| Rabbit Polyclonal DNMT3A Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E |
50 µg /
€395.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA347115 | DNMT3A antibody |
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| Rabbit Polyclonal DNMT3A Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E |
0.1 ml /
€285.00
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|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA349525 | DNMT3A antibody |
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| Rabbit Polyclonal Anti-DNMT3A Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| AP11035PU-N | DNMT3A (C-term) antibody |
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| AP11049PU-N | DNMT3A antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP11050PU-N | DNMT3A antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP11051PU-N | DNMT3A (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP51302PU-N | DNMT3A (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| AP51303PU-N | DNMT3A (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP313064 | DNMT3A (transcript variant 3) |
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| Recombinant protein of human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 3 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
| +1 additional image |
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