DOK3 antibody
Principal name
DOK3 antibody
Alternative names for DOK3 antibody
Docking protein 3, Downstream of tyrosine kinase 3
Ncbi ID
9606, NP_001138347.1, NP_001138347, NM_001144876, NP_079148, NP_079148.2, NP_038767.1, NM_013739, NM_001107336, NM_024872, NM_001144875
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of DOK3 antibody
- Kit Component:
- - KN304748G1, Dok3 gRNA vector 1 in pCas-Guide vector
- KN304748G2, Dok3 gRNA vector 2 in pCas-Guide vector
- KN304748D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Berger, A.H., et al. Nat. Genet. 42(3):216-223 (2010).
Senis, Y.A., et al. J. Thromb. Haemost. 7(10):1718-1726 (2009).
Honma, M., et al. Genes Cells 11(2):143-151 (2006).
Robson, J.D., et al. Mol. Cell. Biol. 24(6):2332-2343 (2004).
Favre, C., et al. Genes Immun. 4(1):40-45 (2003).
DOK3 polyclonal antibody ( Cat # PAB6065 ) staining ( 1 µg/ml ) of human PBMCs lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
DOK3 MaxPab polyclonal antibody. Western Blot analysis of DOK3 expression in human liver.
Western Blot analysis of DOK3 expression in transfected 293T cell line (H00079930-T01) by DOK3 MaxPab polyclonal antibody.Lane 1: DOK3 transfected lysate(36.3 KDa).Lane 2: Non-transfected lysate.
DOK3 MaxPab polyclonal antibody. Western Blot analysis of DOK3 expression in human liver.
Western Blot analysis of DOK3 expression in transfected 293T cell line (H00079930-T01) by DOK3 MaxPab polyclonal antibody.Lane 1: DOK3 transfected lysate(36.3 KDa).Lane 2: Non-transfected lysate.
Formalin fixed and paraffin embedded human lung tissue reacted with DOK3 Antibody (C-term) Cat.-No AP51307PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of DOK3 Antibody (C-term) Cat.-No AP51307PU-N in CEM cell line lysates (35ug/lane). This demonstrates the DOK3 antibody detected the DOK3 protein (arrow).
TA302734 staining (1µg/ml) of Human PBMC lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Sample (30 ug of whole cell lysate). A: A431 . 10% SDS PAGE. TA308614 diluted at 1:1000.
Anti-DOK3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.125 ug/ml.
Anti-DOK3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.125 ug/ml.
Predicted band size: 53 kDa . Positive control: Human notum skin cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using DOK3 antibody at dilution 1/12, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted band size: 53 kDa . Positive control: Human notum skin cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using DOK3 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200)
Western blot analysis of extracts from HepG2 cells, using DOK3 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis on HepG2 cell lysate using DOK3 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA318580 | DOK3 (C-term) antibody |
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| DOK3 Goat Polyclonal (C-Terminus) Antibody | |||||||
 |
Goat | IgG | Aff - Purified | Bov, Hu, Ms, Rt | E, P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA323915 | DOK3 antibody |
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| Anti-DOK3 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA323916 | DOK3 antibody |
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| Anti-DOK3 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
