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DOT1L antibody

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Principal name

DOT1L antibody

Alternative names for DOT1L antibody

Histone H3-K79 methyltransferase, H3-K79-HMTase, DOT1-like protein, Lysine N-methyltransferase 4, KIAA1814, KMT4, Histone-lysine N-methyltransferase H3 lysine-79 specific

SwissProt ID

Q8TEK3 (Human)

Gene ID

84444 (DOT1L)

Ncbi ID

9606, AAI56104.1, NP_115871.1, NM_032482

Available reactivities

Hu (Human)

Available hosts

Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of DOT1L antibody

Parental cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The KO cell lysates are the cell homogenate in RIPA buffer made from the KO cell lines. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control. To facilitate transportation and protein, the products are supplied as lyophilized proteins.

H00084444-M01 - DOT1L
Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody.
TA802479 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802481 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802482 - DOT1L
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802482)
TA802482 - DOT1L
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802482)
TA802482 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802485 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802495 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802738 - DOT1L
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802738)
TA802738 - DOT1L
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802738)
TA802738 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802739 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802740 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802741 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802742 - DOT1L
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802742)
TA802742 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
TA802756 - DOT1L
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI2B10 (formerly 2B10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI2B10 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI5D12 (formerly 5D12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI5D12 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI1D8 (formerly 1D8)  
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802482) Mouse IgG1 OTI1D8 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+2 additional images

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI1F6 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI1F8 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI6E7 (formerly 6E7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802738) Mouse IgG2b OTI6E7 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+2 additional images

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI8A11 (formerly 8A11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI8A11 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI3E9 (formerly 3E9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI3E9 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI11H2 (formerly 11H2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI11H2 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI1F1D4 (formerly 1F1D4)  
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802742) Mouse IgG1 OTI1F1D4 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

DOT1L antibody
  DOT1L mouse monoclonal antibody, clone OTI7H6 (formerly 7H6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI7H6 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Lysates

Catalog No.    

DOT1L overexpression lysate
DOT1L overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L), 200 uL, >10^7 TU/mL
Lenti ORF particles, DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L), 200 uL, >10^7 TU/mL  
200 µl / €2,680.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L), 200 uL, >10^7 TU/mL
Lenti ORF particles, DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L), 200 uL, >10^7 TU/mL  
200 µl / €2,420.00
  OriGene Technologies, Inc.
 

14 Item(s)

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14 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Human Lenti ORF Particle (2)
  2. Lysate (1)
  3. Primary Antibody (11)
Clonality
  1. Monoclonal (11)
Target
  1. DOT1L (11)
Reactivity
  1. Human (Hu) (11)
Applications
  1. Western blot / Immunoblot (WB) (11)
  2. Paraffin Sections (P) (3)
Presentation
  1. Purified (11)
Host
  1. Mouse (11)
Images
  1. 1 image (11)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (11)
  2. Paraffin Sections (P) (3)
Manufacturer
  1. OriGene Technologies (14)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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