DTNB antibody
Principal name
DTNB antibody
Alternative names for DTNB antibody
Dystrobrevin beta, DTN-B, Beta-dystrobrevin
Ncbi ID
9606, NP_149160, AAH16655.1, NP_068707.1, NM_183361, NM_033147, NP_899205, NP_001155937.1, NM_007886, NM_001162465, NM_001012191, NM_183360, NM_021907, NM_033148
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Luminex (LMNX)
Background of DTNB antibody
DTNB is dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin.This gene encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. Alternatively spliced transcript variants encoding different isoforms have been identified.
General readings
Nazarian,R., (2006) Biochem. J. 395 (3), 587-598
DTNB polyclonal antibody (A01), Lot # 060623JCS1 Western Blot analysis of DTNB expression in HepG2 ( Cat # L019V1 ).
Detection limit for recombinant GST tagged DTNB is approximately 1ng/ml as a capture antibody.
DTNB MaxPab polyclonal antibody. Western Blot analysis of DTNB expression in rat brain.
Western Blot analysis of DTNB expression in transfected 293T cell line (H00001838-T01) by DTNB MaxPab polyclonal antibody.Lane 1: DTNB transfected lysate(66.99 KDa).Lane 2: Non-transfected lysate.
DTNB MaxPab polyclonal antibody. Western Blot analysis of DTNB expression in rat brain.
Western Blot analysis of DTNB expression in transfected 293T cell line (H00001838-T01) by DTNB MaxPab polyclonal antibody.Lane 1: DTNB transfected lysate(66.99 KDa).Lane 2: Non-transfected lysate.
Transfected 293T; WB Suggested Anti-DTNB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; DTNB antibody - C-terminal region (ARP46310_P050) in Transfected 293T cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DTNB mouse monoclonal antibody. (TA504258)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DTNB mouse monoclonal antibody. (TA504258)
Anti-DTNB mouse monoclonal antibody (TA504258) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DTNB(RC203798).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTNB (RC203798, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTNB.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DTNB monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTNB (RC203798, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTNB.
HEK293T cells transfected with either RC203798 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DTNB antibody(TA504320), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DTNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504325)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DTNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504325)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTNB (RC203798, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTNB.
HEK293T cells transfected with either RC203798 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DTNB antibody(TA504325), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DTNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504326)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DTNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504326)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DTNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504326)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DTNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504326)
Anti-DTNB mouse monoclonal antibody (TA504326) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DTNB(RC203798).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTNB (RC203798, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTNB.
HEK293T cells transfected with either RC203798 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DTNB antibody(TA504326), and then analyzed by flow cytometry.
Anti-DTNB mouse monoclonal antibody (TA504332) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DTNB(RC203798).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTNB (RC203798, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTNB.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DTNB monoclonal antibody.
HEK293T cells transfected with either RC203798 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DTNB antibody(TA504332), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTNB (RC203798, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTNB.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DTNB monoclonal antibody.
DTNB Luminex Elisa with 3C11 Capture (TA600287) and 3H8 Detection (TA700287) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 2E4 Capture (TA600288) and 3C11 Detection (TA700288) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 2E4 Capture (TA600288) and 3H8 Detection (TA700287) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 2E4 Capture (TA600288) and 2E3 Detection (TA700289) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 3H8 Capture (TA600289) and 3C11 Detection (TA700288) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 3H8 Capture (TA600289) and 2E4 Detection (TA700296) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 3C11 Capture (TA600287) and 3H8 Detection (TA700287) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 2E4 Capture (TA600288) and 3H8 Detection (TA700287) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 2E4 Capture (TA600288) and 3C11 Detection (TA700288) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 3H8 Capture (TA600289) and 3C11 Detection (TA700288) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 2E4 Capture (TA600288) and 2E3 Detection (TA700289) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
DTNB Luminex Elisa with 3H8 Capture (TA600289) and 2E4 Detection (TA700296) Antibodies. Substrate used: Recombinant Human DTNB (TP303798)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (DTNB Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (DTNB Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: 823 cells, Primary antibody: (DTNB Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504258 | DTNB antibody |
||||||
| DTNB (Dystrobrevin beta) mouse monoclonal antibody, clone OTI3C11 (formerly 3C11) | |||||||
 |
Mouse | IgG1 | OTI3C11 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
||||||
| TA504325 | DTNB antibody |
||||||
| DTNB (Dystrobrevin beta) mouse monoclonal antibody, clone OTI2E6 (formerly 2E6) | |||||||
 |
Mouse | IgG1 | OTI2E6 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA504326 | DTNB antibody |
||||||
| DTNB (Dystrobrevin beta) mouse monoclonal antibody, clone OTI3A12 (formerly 3A12) | |||||||
 |
Mouse | IgG1 | OTI3A12 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA504332 | DTNB antibody |
||||||
| DTNB (Dystrobrevin beta) mouse monoclonal antibody, clone OTI3H8 (formerly 3H8) | |||||||
 |
Mouse | IgG2a | OTI3H8 | Purified | Hu, Mky, Ms, Rt | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA349906 | DTNB antibody |
||||||
| Rabbit Polyclonal Anti-DTNB Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349907 | DTNB antibody |
||||||
| Rabbit Polyclonal Anti-DTNB Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA504320 | DTNB antibody |
||||||
| DTNB (Dystrobrevin beta) mouse monoclonal antibody, clone OTI2E4 (formerly 2E4) | |||||||
 |
Mouse | IgG1 | OTI2E4 | Purified | Can, Hu, Mky, Ms, Rt | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA504378 | DTNB antibody |
||||||
| DTNB (Dystrobrevin beta) mouse monoclonal antibody, clone OTI2E3 (formerly 2E3) | |||||||
 |
Mouse | IgG1 | OTI2E3 | Purified | Hu, Mky, Ms | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA600287 | DTNB antibody |
||||||
| Luminex validated DTNB mouse monoclonal antibody (clone 3C1, clone OTI3C11 (formerly 3C11) | |||||||
 |
Mouse | IgG1 | OTI3C11 | Purified | Hu | LMNX |
0.1 mg /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| TA600288 | DTNB antibody |
||||||
| Luminex validated DTNB mouse monoclonal antibody (clone 2E4, clone OTI2E4 (formerly 2E4) | |||||||
 |
Mouse | IgG1 | OTI2E4 | Purified | Hu | LMNX |
0.1 mg /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA600289 | DTNB antibody |
||||||
| Luminex validated DTNB mouse monoclonal antibody (clone 3H8, clone OTI3H8 (formerly 3H8) | |||||||
 |
Mouse | IgG1 | OTI3H8 | Purified | Hu | LMNX |
0.1 mg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA700287 | DTNB antibody |
||||||
| Luminex validated biotinylated DTNB mouse monoclonal antibody (clone 3H8), clone OTI3H8 (formerly 3H8) | |||||||
 |
Mouse | IgG1 | OTI3H8 | Purified | Hu | LMNX |
50 µg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA700288 | DTNB antibody |
||||||
| Luminex validated biotinylated DTNB mouse monoclonal antibody (clone 3C11), clone OTI3C11 (formerly 3C11) | |||||||
 |
Mouse | IgG1 | OTI3C11 | Purified | Hu | LMNX |
50 µg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA700289 | DTNB antibody |
||||||
| Luminex validated biotinylated DTNB mouse monoclonal antibody (clone 2E3), clone OTI2E3 (formerly 2E3) | |||||||
 |
Mouse | IgG1 | OTI2E3 | Purified | Hu | LMNX |
50 µg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| TA700296 | DTNB antibody |
||||||
| Luminex validated biotinylated DTNB mouse monoclonal antibody (clone 2E4), clone OTI2E4 (formerly 2E4) | |||||||
 |
Mouse | IgG1 | OTI2E4 | Purified | Hu | LMNX |
50 µg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303798 | DTNB (transcript variant 5) |
||||
| Recombinant protein of human dystrobrevin, beta (DTNB), transcript variant 5 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY405251 | DTNB overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY409709 | DTNB overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR219289L2V | Lenti ORF particles, Dtnb (GFP-tagged) - Mouse dystrobrevin, beta (Dtnb), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,410.00
|
|
| OriGene Technologies, Inc. | ||
| MR209321L2V | Lenti ORF particles, Dtnb (GFP-tagged) - Mouse dystrobrevin, beta (Dtnb), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,330.00
|
|
| OriGene Technologies, Inc. | ||