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DUSP3 antibody

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Principal name

DUSP3 antibody

Alternative names for DUSP3 antibody

VHR, Dual specificity protein phosphatase 3, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase

SwissProt ID

P51452 (Human), Q9D7X3 (Mouse)

Gene ID

1845 (DUSP3), 72349 (Dusp3)

Ncbi ID

9606, NP_004081, NP_004081.1, NP_082483.1, NM_028207, NM_004090

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of DUSP3 antibody

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases ictivate their target kises by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kise superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kises, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq]

General readings

Alonso, A., Rahmouni, S., Williams, S., van Stipdonk, M., Jaroszewski, L., Godzik, A., Abraham, R.
T., Schoenberger, S. P., Mustelin, T. Tyrosine phosphorylation of VHR phosphatase by
ZAP-70. Nature Immun. 4: 44-48, 2003.
2. Ishibashi, T., Bottaro, D. P., Chan, A., Miki, T., Aaronson, S. A. Expression cloning of a human
dual-specificity phosphatase. Proc. Nat. Acad. Sci. 89: 12170-12174, 1992.
3. Patterson, K. I., Brummer, T., O'Brien, P. M., Daly, R. J. Dual-specificity phosphatases: critical
regulators with diverse cellular targets.Biochem. J. 418: 475-489, 2009.

H00001845-M01 - DUSP3
Western Blot analysis of DUSP3 expression in transfected 293T cell line by DUSP3 monoclonal antibody (M01), clone 5B7.Lane 1: DUSP3 transfected lysate(20.478 KDa).Lane 2: Non-transfected lysate.
H00001845-M01 - DUSP3
Detection limit for recombinant GST tagged DUSP3 is approximately 1ng/ml as a capture antibody.
H00001845-B01 - DUSP3
Immunofluorescence of purified MaxPab antibody to DUSP3 on HeLa cell. [antibody concentration 10 ug/ml]
H00001845-B01 - DUSP3
Western Blot analysis of DUSP3 expression in transfected 293T cell line (H00001845-T01) by DUSP3 MaxPab polyclonal antibody.Lane 1: DUSP3 transfected lysate(20.35 KDa).Lane 2: Non-transfected lysate.
PAB4127 - DUSP3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with DUSP3 polyclonal antibody ( Cat # PAB4127 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PAB4175 - DUSP3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with DUSP3 polyclonal antibody ( Cat # PAB4175 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PAB4175 - DUSP3
Western blot analysis of DUSP3 polyclonal antibody ( Cat # PAB4175 ) in SK-BR-3 cell lysate. DUSP3 ( arrow ) was detected using the purified polyclonal antibody.
MAB0835 - DUSP3
Immunoblot analysis of cell lysates. Lane 1 : HepG2 cell lysate. Lane 2 : Hep3B cell lysate. Lane 3 : U87 mg cell lysate. Lane 4 : 293T cell lysate.
AP15324PU-N - DUSP3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
AP15324PU-N - DUSP3
Western blot analysis of anti-DUSP3 Pab in SK-BR-3 cell line lysate.DUSP3(arrow) was detected using the purified Pab
AR50062PU-N - DUSP3
TP301119 - DUSP3
TA308662 - DUSP3
Sample (50 ug of whole cell lysate). A: mouse stomach. 12% SDS PAGE. TA308662 diluted at 1:1000.
TA308662 - DUSP3
DUSP3 antibody detects DUSP3 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15 % SDS-PAGE, and blotted with DUSP3 antibody (TA308662) diluted by 1:1000
TA308662 - DUSP3
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using DUSP3(TA308662) antibody at 1:500 dilution.
AM50634PU-N - DUSP3
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DUSP3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Jurkat cell lysate Lane 4.: MCF7 cell lysateLane 5.: 293T cell lysateLane 6.: U87MG cell lysateLane 7.: K562 cell lysate

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DUSP3 antibody
  Rabbit Polyclonal antibody to DUSP3 (dual specificity phosphatase 3)  
Sample (50 ug of whole cell lysate). A: mouse stomach. 12% SDS PAGE. TA308662 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

DUSP3 antibody
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DUSP3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Jurkat cell lysate Lane 4.: MCF7 cell lysate Lane 5.: 293T cell lysate Lane 6.: U87MG cell lysate Lane 7.: K562 cell lysate Mouse IgG2b AT9E6 Purified Hu E, WB
0.1 ml / €330.00
  OriGene Technologies GmbH

DUSP3 antibody
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DUSP3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Jurkat cell lysate Lane 4.: MCF7 cell lysate Lane 5.: 293T cell lysate Lane 6.: U87MG cell lysate Lane 7.: K562 cell lysate Mouse IgG2b AT9E6 Purified Hu E, WB
50 µl / €220.00
  OriGene Technologies GmbH

DUSP3 (N-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DUSP3
  Recombinant protein of human dual specificity phosphatase 3 (DUSP3)  
DUSP3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dusp3 (GFP-tagged) - Mouse dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related) (Dusp3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Dusp3 (GFP-tagged) - Mouse dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related) (Dusp3), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Dusp3 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related) (Dusp3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Dusp3 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related) (Dusp3), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

7 Item(s)

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Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (4)
  3. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (2)
  2. Polyclonal (2)
Target
  1. DUSP3 (5)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (2)
  3. Paraffin Sections (P) (2)
Presentation
  1. Purified (3)
  2. Aff - Purified (1)
Host
  1. Mouse (2)
  2. Rabbit (2)
Images
  1. 1 image (5)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. IHC (1)
  3. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (7)
Service
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