DUSP4 antibody
Principal name
DUSP4 antibody
Alternative names for DUSP4 antibody
MKP2, VH2, Dual specificity protein phosphatase 4, Mitogen-activated protein kinase phosphatase 2, Dual specificity protein phosphatase hVH2
Ncbi ID
9606, NP_001385, NP_001385.1, NM_001394, NM_057158, NP_476499, NP_795907.1, NM_176933, NM_022199
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of DUSP4 antibody
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases ictivate their target kises by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kise superfamily (MAPK/ERK; SAPK/JNK; p38); which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kises; different tissue distribution and subcellular localization; and different modes of inducibility of their expression by extracellular stimuli. This gene product ictivates ERK1; ERK2 and JNK; is expressed in a variety of tissues; and is localized in the nucleus. Two altertively spliced transcript variants; encoding distinct isoforms; have been observed for this gene. In addition; multiple polyadenylation sites have been reported.
DUSP4 monoclonal antibody (M01), clone 3D6-G6 Western Blot analysis of DUSP4 expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of DUSP4 expression in transfected 293T cell line by DUSP4 monoclonal antibody (M08), clone 1D12.Lane 1: DUSP4 transfected lysate(43 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged DUSP4 is approximately 3ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with DUSP4 polyclonal antibody ( Cat # PAB4129 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of DUSP4 polyclonal antibody ( Cat # PAB4129 ) in mouse brain tissue lysate ( 35 µg/lane ) . DUSP4 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Proximity Ligation Analysis (PLA) of protein-protein interactions between DUSP4 and MAPK9 HeLa cells were stained with anti-DUSP4 rabbit purified polyclonal 1:1200 and anti-MAPK9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of DUSP4 expression in transfected 293T cell line (H00001846-T01) by DUSP4 MaxPab polyclonal antibody.Lane 1: DUSP4 transfected lysate(43.00 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining
(LEFT)Western blot analysis of anti-DUSP4 Pab (Cat. #AP8447b) in mouse brain tissue lysate (35ug/lane). DUSP4(arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-DUSP4 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). DUSP4 (arrow) was detected using the purified Pab.
Immunoblot analysis of cell lysates.Lane 1 : Bosc23 cell lysate.Lane 2 : Bosc23 cell lysate transfected with Myc/His/Tau.Lane 3 : Bosc23 cell lysate transfected with Myc/His/PK7.Lane 4 : Bosc23 cell lysate transfected with GFP-Ubiquitin+1.
Immunohistochemistry (IHC) analyzes of MKP-2 antibody (CAt.-No.: AP20437PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of MKP-2 antibody (Cat.-No.: AP20437PU-N) in extracts from RAW264.7
Gel: 8%SDS-PAGE, Lysate: 40 µg, Lane: LO2 cells, Primary antibody:DUSP4 Antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using DUSP4 Antibody at dilution 1/40, on the right is treated with fusion protein. (Original magnification:×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using DUSP4 Antibody at dilution 1/40, on the right is treated with fusion protein. (Original magnification:×200)
Predicted band size: 43 kDa . Positive control: RAW264.7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/50-150 The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using DUSP4 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA321338 | DUSP4 antibody |
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| Anti-DUSP4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA321339 | DUSP4 antibody |
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| Anti-DUSP4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP15262PU-N | DUSP4 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP20437PU-N | DUSP4 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300640 | DUSP4 (transcript variant 1) |
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| Recombinant protein of human dual specificity phosphatase 4 (DUSP4), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP315252 | DUSP4 (transcript variant 2) |
||||
| Recombinant protein of human dual specificity phosphatase 4 (DUSP4), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400543 | DUSP4 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY403296 | DUSP4 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209242L2V | Lenti ORF particles, Dusp4 (GFP-tagged ORF) - Rat dual specificity phosphatase 4 (Dusp4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR209242L1V | Lenti ORF particles, Dusp4 (Myc-DDK-tagged ORF) - Rat dual specificity phosphatase 4 (Dusp4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
