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DUT antibody

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Principal name

DUT antibody

Alternative names for DUT antibody

dUTPase, dUTP pyrophosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase mitochondrial

SwissProt ID

P33316 (Human), P33317 (Yeast), P70583 (Rat)

Gene ID

1854 (DUT), 497778 (Dut)

Ncbi ID

9606, NP_001020419, NP_001020419.1, NP_001020420, NP_001939, NM_053592, NM_001040271, NM_001025248, NM_001025249

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Chicken, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of DUT antibody

DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

General readings

Studebaker,A.W., (2005) Biochem. Biophys. Res. Commun. 327 (1), 306-310

H00001854-M01 - DUT
Immunoperoxidase of monoclonal antibody to DUT on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]
H00001854-M01 - DUT
Immunofluorescence of monoclonal antibody to DUT on HeLa cell . [antibody concentration 10 ug/ml]
H00001854-M01 - DUT
Western Blot analysis of DUT expression in transfected 293T cell line by DUT monoclonal antibody (M01), clone 1C9.Lane 1: DUT transfected lysate(18 KDa).Lane 2: Non-transfected lysate.
H00001854-M01 - DUT
Detection limit for recombinant GST tagged DUT is approximately 0.03ng/ml as a capture antibody.
H00001854-B01 - DUT
Western Blot analysis of DUT expression in transfected 293T cell line (H00001854-T01) by DUT MaxPab polyclonal antibody.Lane 1: DUT transfected lysate(27.72 KDa).Lane 2: Non-transfected lysate.
H00001854-D01P - DUT
Western Blot analysis of DUT expression in transfected 293T cell line (H00001854-T01) by DUT MaxPab polyclonal antibody.Lane 1: DUT transfected lysate(26.60 KDa).Lane 2: Non-transfected lysate.
ARP46027_T100 - DUT
Human Heart; DUT antibody - C-terminal region (ARP46027_T100) in Human Heart cells using Immunohistochemistry
ARP46027_T100 - DUT
Human Jurkat; WB Suggested Antibody Titration: 2.5ug/ml. Positive Control: Jurkat. DUT is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; DUT antibody - C-terminal region (ARP46027_T100) in Human Jurkat cells using Western Blot
AP09036AF-N - DUT
Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE)
AP09036AF-N - DUT
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
H00001854-D01 - DUT
Western Blot analysis of DUT expression in transfected 293T cell line (H00001854-T01) by DUT MaxPab polyclonal antibody.Lane 1: DUT transfected lysate(26.60 KDa).Lane 2: Non-transfected lysate.
H00001854-D01 - DUT
Immunoprecipitation of DUT transfected lysate using anti-DUT MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DUT MaxPab mouse polyclonal antibody (B01) (H00001854-B01).
ARP46028_P050 - DUT
Human Jurkat; Immunohistochemistry with Jurkat cell lysate tissue; DUT antibody - N-terminal region (ARP46028_P050) in Human Jurkat cells using Immunohistochemistry
ARP46028_P050 - DUT
Human Fetal Small Intestine; WB Suggested Anti-DUT Antibody Titration: 1 ug/ml. Positive Control: Fetal Small Intestine cell lysate; DUT antibody - N-terminal region (ARP46028_P050) in Human Fetal Small Intestine cells using Western Blot
AR09663PU-L - DUT
AR09663PU-L - DUT
Recombinant human DUT, 70-252 aa, His-tagged
AP23256PU-N - DUT
Immunohistochemical analysis of paraffin embedded rat tissue sections (cardiac muscle), staining DUTPase in cytoplasm, DAB chromogenic reaction
AM20882PU-N - DUT
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20882PU-N - DUT
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20882PU-N - DUT
Immunofluorescence of monoclonal antibody to DUT on HeLa cell (antibody concentration 10 µg/ml).
AM20882PU-N - DUT
Western Blot analysis of DUT expression in transfected 293T cell line by DUT monoclonal antibody clone 1C9.
AM20882PU-N - DUT
Detection limit for recombinant GST tagged DUT is approximately 0.03 ng/ml as a capture antibody.
TP310600 - DUT
TP321635 - DUT
AP33499PU-N - DUT
AP33499PU-N (0.1µg/ml) staining of HEK293 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
TA321411 - DUT
Predicted band size: 27 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/200-1000
TA321412 - DUT
Predicted band size: 27 kDa. Positive control: 293T cell lysate. Recommended dilution: 1/200-1000
TA321412 - DUT
Predicted cell location: Cytoplasm . Positive control: Human liver cancer tissue. Recommended dilution: 1/10-100 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using DUT antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification: x200)
TA338974 - DUT
WB Suggested Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat.DUT is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
TA338974 - DUT
Human Heart
TA338975 - DUT
WB Suggested Anti-DUT Antibody Titration: 1 ug/ml; Positive Control: Fetal Small Intestine cell lysate
TA338975 - DUT
Immunohistochemistry with Jurkat cell lysate tissue

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DUT antibody
  Anti-DUT Rabbit Polyclonal Antibody  
Predicted band size: 27 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

DUT antibody
  Anti-DUT Rabbit Polyclonal Antibody  
Predicted band size: 27 kDa. Positive control: 293T cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

DUT (1-52) antibody
Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Chicken IgY Azide Free Hu P, WB
50 µg / €475.00
  OriGene Technologies GmbH
+1 additional image

DUT antibody
Immunohistochemical analysis of paraffin embedded rat tissue sections (cardiac muscle), staining DUTPase in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €390.00
  OriGene Technologies GmbH

DUT antibody
AP33499PU-N (0.1µg/ml) staining of HEK293 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Bov, Hu, Por WB
0.1 mg / €380.00
  OriGene Technologies GmbH

5 Item(s)

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  1. Primary Antibody (5)
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