Dynamin-1 antibody
Principal name
Dynamin-1 antibody
Alternative names for Dynamin-1 antibody
DNM1, DNM
Ncbi ID
NP_001005336.1, NP_542420.1, 9606, NP_001005336, NP_004399, NM_004408, NM_001005336, NP_034195.2, BC034679, NM_010065, NM_080689
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of Dynamin-1 antibody
- Kit Component:
- - KN304717G1, Dnm1 gRNA vector 1 in pCas-Guide vector
- KN304717G2, Dnm1 gRNA vector 2 in pCas-Guide vector
- KN304717D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Mori H et al. Transmembrane/cytoplasmic, rather than catalytic, domains of Mmp14 signal to MAPK activation and mammary branching morphogenesis via binding to integrin ß1. Development 140:343-52 (2013). WB ; Mouse .
2. Martins-de-Souza D et al. Phosphoproteomic differences in major depressive disorder postmortem brains indicate effects on synaptic function. Eur Arch Psychiatry Clin Neurosci 262:657-66 (2012). WB ; Human .
Immunohistochemistry analyzes of Dynamin-1 antibody (Cat.-No.: AP06385PU-N) in Paraffin-Embedded Human tbrain tissue.
Western blot (WB) analysis of Dynamin-1 antibody (Cat.-No.: AP06385PU-N) in extracts from PC12 cells.
AP08309PU-N Dynamin-1 antibody staining of Formalin-Fixed Paraffin Embedded Human Brain, Cortex at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
Western blot of rat cortex lysate showing specific immunolabeling of the ~95k Dynamin protein. Using Dynamin polyclonal antibody ( Cat # PAB9596 ).
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Dynamin (phospho S774) polyclonal antibody ( Cat # PAB9697 ). The immunolabeling is completely eliminated by treatment with l-Ptase.
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser778 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase, lane 2). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Dynamin (phospho S778) polyclonal antibody ( Cat # PAB9698 ). The immunolabeling is completely eliminated by treatment with l-Ptase.
Figure 1 Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser774 Dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Figure 1 Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 Dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Mouse Brain; Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science. Application: IHC. Species+tissue/cell type: Mouse brain stem cells. Primary antibody dilution: 1:500. Secondary antibody: Goat anti-rabbit Alexa-Fluor 594. Secondary antibody dilution: 1:1000; DNM1 antibody - middle region (ARP51787_P050) in Mouse Brain cells using Immunohistochemistry
Rat Brain; . . Sample Type. . : 3. rat brain extract (80ug). Primary Antibody Dilution: 2ug/ml. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Secondary Antibody Dilution: 1: 20,000. Image Submitted by: Yuzhi Chen. University of Arkansas for Medical Science .; DNM1 antibody - middle region (ARP51787_P050) in Rat Brain cells using Western Blot
Human brain; WB Suggested Anti-DNM1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human brain; DNM1 antibody - middle region (ARP51787_P050) in Human brain cells using Western Blot
Immunohistochemical analysis of paraffin-embedded human lymph tissue (A), glioma tissue (B) and cerebellum tissue (C), showing membrane localization using Dynamin-1 antibody Cat.-No AM06123SU-N with DAB staining
Western blot analysis using Dynamin-1 antibody Cat.-No AM06123SU-N against C6 (Lane 1), NIH/3T3 (Lane 2), SKN-SH (Lane 3), LN18 (Lane 4), SHSY5Y (Lane 5) cell lysate and rat brain tiisues lysate (Lane 6).
Western blot analysis of Dynamin 1 (pS774) expression in HeLa (A); NIH3T3 (B); PC12 (C) whole cell lysates.
Western Blot of rat cortex lysate showing labeling of the ~95k Dynamin protein.
Western blot analysis of extracts from HeLa cells, using DYN1 (Ab-4520) antibody.The lane on the right is treated with the synthesized peptide.
Gel: 8%SDS-PAGE, Lysate: 30 µg, Lane: Mouse brain tissue, Primary antibody: DNM1 Antibody at dilution 1/100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 8%SDS-PAGE, Lysate: 40 µg, Lane: Mouse brain tissue, Primary antibody: DNM1 Antibody at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Western blot analysis of Dynamin-1 expression in Mouse braintissue lysates,The lane on the left is treated with the antigen-specific peptide.
Western Blot: Dynamin Antibody (3G4B6) [TA336537] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.
Western Blot: Dynamin Antibody (3G4B6) [TA336537] - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).
Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [TA336537] - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using Dynamin antibody with DAB staining.
Flow Cytometry: Dynamin Antibody (3G4B6) [TA336537] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was
Western blot analysis of extracts from Hela cells using Dynamin 1 Antibody
Western blot analysis of Dynamin-1 phosphorylation expression in Mouse braintissue lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment with λ-Ptase.
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with λ-Ptase.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA336537 | Dynamin-1 antibody |
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| Mouse Monoclonal Dynamin Antibody (3G4B6) | |||||||
![Western Blot: Dynamin Antibody (3G4B6) [TA336537] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB110-60491-1-W.jpg) |
Mouse | IgG2a | 3G4B6 | Purified | Hu | C, E, F, P, WB |
0.1 ml /
€395.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
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| AM06123SU-N | Dynamin-1 antibody |
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 |
Mouse | IgG2a | 3G4B6 | Ascites | Hu | P, WB |
0.1 ml /
€470.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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