Dysbindin antibody
Principal name
Dysbindin antibody
Alternative names for Dysbindin antibody
Dystrobrevin-binding protein 1, DTNBP1, My031, Hps7-like protein, Hermansky-Pudlak syndrome 7 protein homolog
Ncbi ID
10090, 9606, NP_115498, NM_032122, NM_183040, NP_115498.2, NP_080048.2, NM_001037664
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of Dysbindin antibody
Dysbindin, or dystrobrevin-binding protein 1, is a coiled-coil-containing protein expressed in muscle and brain that was identified as a binding partner of dystrobrevin. Dysbindin upregulates expression of the pre-syptic proteins SP25 and sypsin I, thereby increasing glutamate release and promoting neurol viability through Akt sigling. In particular, Akt phosphorylation is suppressed with downregulation of dysbindin and increased with upregulation of dysbindin. A nonsense mutation of dysbindin causes Hermansky-Pudlak disease, an autosomal recessive disorder characterized by lysosomal storage defects and prolonged bleeding. Genetic variation in the gene encoding dysbindin is strongly associated with schizophrenia and protein levels are reduced in the prefrontal cortex, midbrain and hippocampus of brains from patients with schizophrenia.
General readings
Haukvik, U.K., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry 34(7):1259-1265(2010).
Voisey, J., et al. Eur. Psychiatry 25(6):314-319 (2010).
Ruano, G., et al. Pharmacogenomics 11(7):959-971 (2010).
Hashimoto, R., et al. Nihon Shinkei Seishin Yakurigaku Zasshi 30(3):103-107 (2010).
Voisey, J., et al. Behav Brain Funct 6, 41 (2010).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Dtnbp1 polyclonal antibody ( Cat # PAB2552 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of Dtnbp1 ( arrow ) using rabbit Dtnbp1 polyclonal antibody ( Cat # PAB2552 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the Dtnbp1 gene ( Lane 2 ) ( Origene Technologies ) .
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Dtnbp1 polyclonal antibody ( Cat # PAB2553 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of Dtnbp1 ( arrow ) using rabbit Dtnbp1 polyclonal antibody ( Cat # PAB2553 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the Dtnbp1 gene ( Lane 2 ) ( Origene Technologies ) .
Western Blot analysis of DTNBP1 expression in transfected 293T cell line (H00084062-T01) by DTNBP1 MaxPab polyclonal antibody.Lane 1: DTNBP1 transfected lysate(38.61 KDa).Lane 2: Non-transfected lysate.
Recombinant human Dysbindin, 1-270 aa, His-tagged: 15% SDS-PAGE (3 µg)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Dtnbp1 Antibody (N-term) (Cat.#AP12328PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of Dtnbpt (arrow) using rabbit polyclonal Dysbindin/Dtnbp1 Antibody (N-term) (Cat.#AP12328PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Dtnbpt gene (Lane 2) (Origene Technologies).
Western Blot analysis of DTNBP1 expression in transfected 293T cell line (H00084062-T02) by DTNBP1 MaxPab polyclonal antibody.Lane 1: DTNBP1 transfected lysate(39.50 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of DTNBP1 (arrow) using Dysbindin Antibody (N-term) Cat.-No AP51331PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DTNBP1 gene.
Western blot analysis of Dysbindin Antibody (N-term) Cat.-No AP51331PU-N in Hela cell line lysates (35ug/lane). This demonstrates the DTNBP1 antibody detected the DTNBP1 protein (arrow).
Western blot analysis of Dtnbp1(arrow) using rabbit polyclonal Dysbindin(Dtnbp1) Antibody (C-term) (Cat.#TA300078).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Dtnbp1 Antibody (C-term) (Cat.#TA300078), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Predicted cell location: Cytoplasm. Positive control: Human brain and thyroid cancer tissue. Recommended dilution: 1/20-100 The image on the left is immunohistochemistry of paraffin-embedded Human brain and thyroid cancer tissue using DTNBP1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human brain and thyroid cancer tissue. Recommended dilution: 1/20-100 The image on the left is immunohistochemistry of paraffin-embedded Human brain and thyroid cancer tissue using DTNBP1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of extracts of various cell lines, using DTNBP1 antibody.
Immunohistochemistry of paraffin-embedded human esophageal cancer using DTNBP1 antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded rat lung using DTNBP1 antibody at dilution of 1:100 (200x lens).
Immunofluorescence analysis of MCF7 cell using DTNBP1 antibody. Blue: DAPI for nuclear staining.
Host: Rabbit; Target Name: DTNBP1; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA327114 | Dysbindin antibody |
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| DTNBP1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA300078 | Dysbindin (C-term) antibody |
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| Rabbit Polyclonal Antibody against DTNBP1 | |||||||
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Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321915 | Dysbindin antibody |
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| Anti-DTNBP1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP12328PU-N | Dysbindin (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP51331PU-N | Dysbindin (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304208 | Dysbindin (transcript variant 1) |
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| Recombinant protein of human dystrobrevin binding protein 1 (DTNBP1), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403167 | DTNBP1 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. | ||
| LY405212 | DTNBP1 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. | ||
| LY430642 | DTNBP1 overexpression lysate |
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0.1 mg /
€315.00
|
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| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201504L2V | Lenti ORF particles, Dtnbp1 (GFP-tagged ORF) - Rat distrobrevin binding protein 1 (Dtnbp1), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| RR201504L1V | Lenti ORF particles, Dtnbp1 (Myc-DDK-tagged ORF) - Rat distrobrevin binding protein 1 (Dtnbp1), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||