Home » Target » ECT2 antibody

ECT2 antibody

Principal name

ECT2 antibody

Alternative names for ECT2 antibody

Epithelial cell-transforming sequence 2 oncogene

SwissProt ID

Q07139 (Mouse), Q9H8V3 (Human)

Gene ID

1894 (ECT2)

Ncbi ID

9606, NP_060568, NP_060568.3, AAH06838.1, NM_018098

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Chimp (Chimpanzee), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of ECT2 antibody

ECT2 is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of ECT2 is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Justilien, V., et al. Oncogene 28(41):3597-3607(2009).
Burkard, M.E., et al. PLoS Biol. 7 (5), E1000111 (2009).
Wolfe, B.A., et al. PLoS Biol. 7 (5), E1000110 (2009).
Hirata, D., et al. Clin. Cancer Res. 15(1):256-266(2009).
Seguin, L., et al. Mol. Cell. Biol. 29(2):570-581(2009).

Human HeLa; WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate; ECT2 antibody - middle region (ARP52381_P050) in Human HeLa cells using Western Blot

ARP52381_P050

Human HepG2; WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ECT2 antibody - middle region (ARP52382_P050) in Human HepG2 cells using Western Blot

ARP52382_P050

Western blot using ECT2 (phospho T790) polyclonal antibody ( Cat # PAB10006 ) showsdetection of endogenous phospho-ECT2 ( arrowhead ) present in cell lysates from interphase ( lane 1 ) and mitotic ( lane 2 ) HeLa cells.Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA resultsusing both phosphorylated and non-phosphorylated immunizing peptide.After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 1,000.Personal Communication, Toru Miki,CCR-NCI, Bethesda, MD.

PAB10006

Western blot using affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and nonphosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000.

AP09324PU-N

Western blot analysis of ECT2 Antibody Cat.-No AP51360PU-N in 293 cell line lysates (35ug/lane). This demonstrates the ECT2 antibody detected the ECT2 protein (arrow).

AP51360PU-N

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECT2 (RC211319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECT2.

TA590497

ELISA: ECT2 Antibody

TA590497

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ECT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807408)

TA807408

TA807408

WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysate

TA340187

WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate

TA340188

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA319414	ECT2 antibody
	Rabbit polyclonal ECT2 phospho T790 antibody (Phospho-specific)
	Rabbit	IgG		Purified	Can, Chimp, Chk, Hu, Ms, Rt, Ze	E, WB	0.1 mg / €515.00
							OriGene Technologies, Inc.
TA590497	ECT2 antibody
	Rabbit Polyclonal ECT2 Antibody
	Rabbit	IgG		Purified	Hu	E, WB	50 µg / €409.00
							OriGene Technologies, Inc.
+1 additional image
TA807408	ECT2 antibody
	ECT2 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)
	Mouse	IgG1	OTI2D9	Purified	Hu	P, WB	0.1 ml / €360.00
							OriGene Technologies, Inc.
+1 additional image
AP16137PU-N	ECT2 (N-term) antibody
	Goat			Aff - Purified	Hu	WB	0.1 mg / €380.00
							OriGene Technologies GmbH
AP51360PU-N	ECT2 (Center) antibody
	Rabbit	Ig		Aff - Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies GmbH