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EEF-2 kinase antibody

Principal name

EEF-2 kinase antibody

Alternative names for EEF-2 kinase antibody

Elongation factor 2 kinase, EEF2K, EF2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

SwissProt ID

O00418 (Human), O08796 (Mouse), P70531 (Rat)

Gene ID

101930123 (LOC101930123), 29904 (EEF2K), 13631 (Eef2k), 25435

Ncbi ID

NP_037434.1, 9606, NP_037434, NM_013302, NM_007908, NM_012947

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of EEF-2 kinase antibody

The activity of the purified eukaryotic elongation-factor-2 kinase (eEF2K) is completely dependent on calcium and calmodulin, and autophosphorylation on serine and threonine residues is calcium/calmodulin-dependent. eEF2K is a ubiquitous protein kinase that phosphorylates and inactivates eEF2, and thus can modulate the rate of polypeptide chain elongation during translation. eEF2K is 120 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38g, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.

General readings

Ryazanov A.G., et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94:4884-4889Knebel A., et al. (2001) EMBO J. 20:4360-4369

Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using EEF-2 kinase antibody (Cat.-No. AP06095PU-N).

AP06095PU-N

Western blot (WB) analysis of eEF2K antibody (Cat.-No.: AP06095PU-N) at 1/500 dilution
Lane1:Hela cell lysate
Lane2:MCF-7 cell lysate
Lane3:Rat kidney tissue lysate

AP06095PU-N

EEF2K MaxPab polyclonal antibody. Western Blot analysis of EEF2K expression in human colon.

H00029904-B01P

Western Blot analysis of EEF2K expression in transfected 293T cell line (H00029904-T01) by EEF2K MaxPab polyclonal antibody.Lane 1: EEF2K transfected lysate(79.75 KDa).Lane 2: Non-transfected lysate.

H00029904-B01P

EEF2K MaxPab polyclonal antibody. Western Blot analysis of EEF2K expression in HepG2.

H00029904-B01P

Western blot (WB) analysis of eEF2K antibody (Cat.-No.: AP21133PU-N) in extracts from Jurkat cells.

AP21133PU-N

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2K (Phospho-Ser366) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells treated with serum 10% 15', using eEF2K (Phospho-Ser366) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using eEF2K (Ab-366) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, treated with serum 10% 15', using eEF2K (Ab-366) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, using eEF2K (Ab-359) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of EEF2K (pS366) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of EEF2K (pS366) expression in HeLa Calyculin A-treated (A) whole cell lysates.

Immunohistochemical analysis of EEF2K staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of EEF2K expression in K562 (A); MCF7 (B); rat kidney (C) whole cell lysates.

TP307496

Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308475 diluted at 1:1000.

TA308475

Sample (30 ug of whole cell lysate). A: A431 . B: H1299. C: Hela. D: Hep G2 . 7.5% SDS PAGE. TA308475 diluted at 1:5000.

TA308475

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using eEF-2K(TA308475) antibody at 1:500 dilution.

TA308475

Immunofluorescence analysis of paraformaldehyde-fixed A431, using eEF-2K(TA308475) antibody at 1:200 dilution.

TA308475

Western blot analysis of extracts of various cell lines, using EEF2K antibody.

TA332670

Immunofluorescence analysis of MCF-7 cell using EEF2K antibody. Blue: DAPI for nuclear staining.

TA332670

Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (EEF2K Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds

TA349921

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA308475	EEF-2 kinase antibody
	Rabbit Polyclonal antibody to EEF2K (eukaryotic elongation factor-2 kinase)
	Rabbit	IgG	Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 ml / €415.00
						OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
TA332670	EEF-2 kinase antibody
	Rabbit anti-EEF2K Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt		0.1 mg / €335.00
						OriGene Technologies, Inc.
+1 additional image
TA349921	EEF-2 kinase antibody
	Rabbit Polyclonal Anti-EEF2K Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
AP06095PU-N	EEF-2 kinase antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
						OriGene Technologies GmbH
+1 additional image
AP21133PU-N	EEF-2 kinase antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	WB	0.1 mg / €370.00
						OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP307496	EEF-2 kinase
	Recombinant protein of human eukaryotic elongation factor-2 kinase (EEF2K)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY402241	EEF2K overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR210301L2V	Lenti ORF particles, Eef2k (GFP-tagged) - Mouse eukaryotic elongation factor-2 kinase (Eef2k), 200 uL, >10^7 TU/mL
		200 µl / €1,500.00
		OriGene Technologies, Inc.

MR210301L1V	Lenti ORF particles, Eef2k (Myc-DDK-tagged) - Mouse eukaryotic elongation factor-2 kinase (Eef2k), 200 uL, >10^7 TU/mL
		200 µl / €1,350.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR210671L2V	Lenti ORF particles, Eef2k (GFP-tagged ORF) - Rat eukaryotic elongation factor-2 kinase (Eef2k), 200 uL, >10^7 TU/mL
		200 µl / €1,500.00
		OriGene Technologies, Inc.

RR210671L1V	Lenti ORF particles, Eef2k (Myc-DDK-tagged ORF) - Rat eukaryotic elongation factor-2 kinase (Eef2k), 200 uL, >10^7 TU/mL
		200 µl / €1,350.00
		OriGene Technologies, Inc.

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