EGR2 antibody
Principal name
EGR2 antibody
Alternative names for EGR2 antibody
Early growth response protein 2, EGR-2, AT591, KROX20, Krox 20, Zinc finger protein Krox-20
Ncbi ID
NP_000390, 9606, NP_000390.2, NM_001136177, NM_001136178, NM_001136179, NP_001129649, NM_053633, NM_000399
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of EGR2 antibody
EGR2 (E3 SUMO-protein ligase EGR2) protein is a member of EGR C2H2-type zinc-finger family and is a sequence-specific D-binding transcription factor that binds to two specific D sites located in HOXA4 promotor. EGR2 is an E3 SUMO-protein ligase that facilitates SUMO1 conjugation to its coregulators B1/B2, whose sumoylation down-regulates EGR2's own transcriptiol activity. EGR2 interacts with HCFC1, UBC9 and WWP2, and WWP2 mediated ubiquitinization of EGR2 results in its proteasomal degradation. EGR2 protein activates several genes which are implicated in the formation and maintence of myelin, the fatty substance that covers and protects nerve cells. EGR2 is considered a "master" transcription factor that regulates PNS myelition and plays a key role in myelin homeostasis. Besided peripheral nerve myelition, EGR2 plays important role in T cell maturation, hindbrain segmentation, and lipid biosynthesis. Genetic alteration of EGR2 results in a variety of relatively severe demyeliting neuropathies such as congenital hypomyeliting neuropathy (CHN), childhood-onset Dejerine-Sottas neuropathy (DSN), and Charcot-Marie-Tooth disease type 1D (CMT1D).
General readings
Szigeti,K., (2007) Neurogenetics 8 (4), 257-262
Human Lung; . Rabbit Anti-EGR2 Antibody. . Catalog Number:. P100880 . Paraffin Embedded Tissue: Human bronchiole epithelium . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Human Intestine; EGR2 antibody - C-terminal region (P100880_P050) in Human Intestine cells using Immunohistochemistry
Human Lung; WB Suggested Anti-EGR2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Lung; EGR2 antibody - C-terminal region (P100880_P050) in Human Lung cells using Western Blot
EGR2 polyclonal antibody ( Cat # PAB7314 ) ( 0.03 µg/ml ) staining of HepG2 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of EGR2 expression in transfected 293T cell line (H00001959-T01) by EGR2 MaxPab polyclonal antibody.Lane 1: EGR2 transfected lysate(52.36 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to EGR2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of EGR2 expression in transfected 293T cell line (H00001959-T02) by EGR2 MaxPab polyclonal antibody.Lane 1: EGR2 transfected lysate(52.36 KDa).Lane 2: Non-transfected lysate.
EGR2 monoclonal antibody (M03), clone 1G5. Western Blot analysis of EGR2 expression in rat brain.
Western Blot analysis of EGR2 expression in transfected 293T cell line (H00001959-T01) by EGR2 MaxPab polyclonal antibody.Lane 1: EGR2 transfected lysate(50.30 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of EGR2 in human fetal lung tissue using EGR2 polyclonal antibody ( Cat # PAB12439 ).
Human Colon (formalin-fixed, paraffin-embedded) stained with EGR2 antibody AP22550PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Small Intestine (formalin-fixed, paraffin-embedded) stained with EGR2 antibody AP22550PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody (0.03 µg/ml) staining of HepG2 cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-EGR2 mouse monoclonal antibody (TA505319) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EGR2(RC212183).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGR2 (RC212183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGR2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EGR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505382)
Anti-EGR2 mouse monoclonal antibody (TA505382) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EGR2(RC212183).
Immunofluorescent staining of HeLa cells using anti-EGR2 mouse monoclonal antibody (TA505382) at 1:100(1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGR2 (RC212183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGR2.
TA303254 (0.03µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from K562 cells, using EGR2 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-EGR2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Lung
Human Intestine
Rabbit Anti-EGR2 Antibody; Paraffin Embedded Tissue: Human bronchiole epithelium; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Western Blot: EGR2 Antibody [TA336535] - Detection of EGR2 in human fetal lung tissue using TA336535.
Immunocytochemistry/Immunofluorescence: EGR2 Antibody [TA336535] - EGR2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western blot analysis on HeLa cell lysate using EGR2 Antibody
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505382 | EGR2 antibody |
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| EGR2 mouse monoclonal antibody, clone OTI1B12 (formerly 1B12) | |||||||
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Mouse | IgG1 | OTI1B12 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Top Selling product! Highly validated antibody!
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| TA303254 | EGR2 antibody |
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| Goat Polyclonal Antibody against EGR2 | |||||||
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Goat | Aff - Purified | Can, Hu, Ms, Rt | E, P, WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA326135 | EGR2 antibody |
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| Rabbit polyclonal EGR2 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA336535 | EGR2 antibody |
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| Rabbit Polyclonal EGR2 Antibody | |||||||
![Western Blot: EGR2 Antibody [TA336535] - Detection of EGR2 in human fetal lung tissue using TA336535.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB110-59723-1-W.jpg) |
Rabbit | Purified | Hu, Por | ICC/IF, WB |
0.1 ml /
€395.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA347615 | EGR2 antibody |
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| Rabbit Polyclonal Anti-EGR2 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA505319 | EGR2 antibody |
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| EGR2 mouse monoclonal antibody, clone OTIF10 (formerly F10) | |||||||
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Mouse | IgG2b | OTIF10 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP22550PU-N | EGR2 (239-251) antibody |
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Goat | Aff - Purified | Bov, Can, Elephant, Eq, Hu, Mky, Por, Rb | E, P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP54904SU-N | EGR2 antibody |
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Rabbit | Serum | Ms | C, E, WB |
0.2 ml /
€430.00
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| OriGene Technologies GmbH | |||||||
| RA25024-100 | EGR2 (200-300) antibody |
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Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€380.00
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| Neuromics Antibodies | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760752 | EGR2 (full length, N-term HIS tag, transcript variant 2) |
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| Purified recombinant protein of Human early growth response 2 (EGR2), transcript variant 2, full length, with N-terminal HIS tag, expressed in E. coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY427835 | EGR2 overexpression lysate |
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0.1 mg /
€315.00
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| OriGene Technologies, Inc. | ||
| LY427836 | EGR2 overexpression lysate |
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0.1 mg /
€315.00
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| OriGene Technologies, Inc. | ||
| LY427837 | EGR2 overexpression lysate |
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0.1 mg /
€315.00
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| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR206025L2V | Lenti ORF particles, Egr2 (GFP-tagged ORF) - Rat early growth response 2 (Egr2), 200 uL, >10^7 TU/mL |
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200 µl /
€1,150.00
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| OriGene Technologies, Inc. | ||
| RR206025L1V | Lenti ORF particles, Egr2 (Myc-DDK-tagged ORF) - Rat early growth response 2 (Egr2), 200 uL, >10^7 TU/mL |
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200 µl /
€1,020.00
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| OriGene Technologies, Inc. | ||
