EHD2 antibody
Principal name
EHD2 antibody
Alternative names for EHD2 antibody
PAST2, EH domain-containing protein 2, PAST homolog 2
Ncbi ID
9606, NP_055416.2, NP_055416, NP_694708.2, BC027084, NM_153068, NM_001024897, NM_014601
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)
Background of EHD2 antibody
- Kit Component:
- - KN305073G1, Ehd2 gRNA vector 1 in pCas-Guide vector
- KN305073G2, Ehd2 gRNA vector 2 in pCas-Guide vector
- KN305073D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence of monoclonal antibody to EHD2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of EHD2 expression in transfected 293T cell line by EHD2 monoclonal antibody (M01), clone 2D8.Lane 1: EHD2 transfected lysate(61.2 KDa).Lane 2: Non-transfected lysate.
EHD2 monoclonal antibody (M01), clone 2D8 Western Blot analysis of EHD2 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged EHD2 is approximately 0.03ng/ml as a capture antibody.
EHD2 polyclonal antibody ( Cat # PAB6684 ) staining ( 0.003 µg/ml ) of human heart lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing EHD2 and probed with EHD2 polyclonal antibody ( Cat # PAB6684 ) ( mock transfection in first lane ), tested by Origene.
EHD2 monoclonal antibody (M02), clone 1F1. Western Blot analysis of EHD2 expression in HeLa(Cat # L013V1 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EHD2 (RC204848, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EHD2.
ELISA: EHD2 Antibody
TA302472 staining (0.003µg/ml) of human heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302472 | EHD2 (C-term) antibody |
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| Goat Polyclonal Antibody against EHD2 | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt, Ze | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA590248 | EHD2 antibody |
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| Rabbit Polyclonal EHD2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304848 | EHD2 |
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| Recombinant protein of human EH-domain containing 2 (EHD2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207060L2V | Lenti ORF particles, Ehd2 (GFP-tagged) - Mouse EH-domain containing 2 (cDNA clone MGC:38650 IMAGE:5356224), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR220542L2V | Lenti ORF particles, Ehd2 (GFP-tagged) - Mouse EH-domain containing 2 (Ehd2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR207060L1V | Lenti ORF particles, Ehd2 (Myc-DDK-tagged) - Mouse EH-domain containing 2 (cDNA clone MGC:38650 IMAGE:5356224), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR220542L1V | Lenti ORF particles, Ehd2 (Myc-DDK-tagged) - Mouse EH-domain containing 2 (Ehd2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212003L2V | Lenti ORF particles, Ehd2 (GFP-tagged ORF) - Rat EH-domain containing 2 (Ehd2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,250.00
|
|
| OriGene Technologies, Inc. | ||
| RR212003L1V | Lenti ORF particles, Ehd2 (Myc-DDK-tagged ORF) - Rat EH-domain containing 2 (Ehd2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||