EIF4B antibody
Principal name
EIF4B antibody
Alternative names for EIF4B antibody
Eukaryotic translation initiation factor 4B, eIF-4B
Ncbi ID
NP_001408, 9606, NP_001408.2, NP_663600.2, BC007171, NM_145625, NM_001417
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))
Background of EIF4B antibody
Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kises, including p70 S6 kise, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6). Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens (7), and the phosphorylation of this site is dependent on MEK and mTOR activity (7). This phosphorylation is shown to be essential for the translatiol activity of eIF4B (7).
General readings
Suzuki,Y., (2004) Genome Res. 14 (9), 1711-1718
Human Heart; Rabbit Anti-EIF4B Antibody. Catalog Number: ARP40359. Paraffin Embedded Tissue: Human Heart. Cellular Data: Myocardial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; EIF4B antibody - C-terminal region (ARP40359_T100) in Human Heart cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-EIF4B Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. EIF4B is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; EIF4B antibody - C-terminal region (ARP40359_T100) in Human Jurkat cells using Western Blot
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B polyclonal antibody ( Cat # PAB3774 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of EIF4B polyclonal antibody ( Cat # PAB3774 ) in 293 cell line lysates transiently transfected with the EIF4B gene ( 2 µg/lane ) . EIF4B ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western blot analysis of EIF4B polyclonal antibody ( Cat # PAB3774 ) in HepG2 cell line lysates ( 35 µg/lane ) . EIF4B ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B polyclonal antibody ( Cat # PAB3951 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of EIF4B ( arrow ) using rabbit EIF4B polyclonal antibody ( Cat # PAB3951 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the EIF4B gene ( Lane 2 ) ( Origene Technologies ) .
Immunohistochemistry (IHC) analyzes of eIF4B pSer422 antibody (Cat.-No.: AP01821PU-N) in paraffin-embedded human colon carcinoma tissue.
Western blot (WB) analysis of eIF4B pSer422 antibody (Cat.-No.: AP01821PU-N) in extracts from NIH/3T3 cells.
AP11771PU-N EIF4B antibody staining of Formalin-Fixed, Paraffin-Embedded Human breast carcinoma. This antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis using AP11771PU-N EIF4B Antibody (Center) in HepG2 cell line lysates (35 µg/lane). EIF4B (arrow) was detected using the purified Pab at 1/60 dilution.
Western blot analysis using AP11771PU-N EIF4B Antibody (Center) in 293 cell line lysates transiently transfected with the EIF4B gene (2 µg/lane). EIF4B (arrow) was detected using the purified Pab at 1/60 dilution.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (AP11772PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4B gene (Lane 2) (Origene Technologies).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using EIF4B polyclonal antibody ( Cat # PAB12256 ).
Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B pSer422 antibody (AP09472PU) (Left) and the same antibody preincubated with blocking peptide (Right).
Figure 2. Western blot analysis of extracts from Hela cells untreated or treated with EGF using eIF4B pSer422 Antibody (AP09472PU).
Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B Antibody (#AP09474PU) (Left) or the same antibody preincubated with blocking peptide (Right).
Figure 2. Western blot analysis of extract from 3T3 and HL60 cells using eIF4B Antibody (#AP09474PU).
Immunofluorescence analysis of NIH3T3 cells using EIF4B antibbody Cat.-No AM06769PU-N EIF4B (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using EIF4B antibody Cat.-No AM06769PU-N against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)
Western blot analysis using EIF4B antibody Cat.-No AM06769PU-N against A549 (Lane 1), A431 (Lane 2), HepG2 (Lane 3), HEK293 (Lane 4), HeLa (Lane 5), Jurkat (Lane 6), K562 (Lane 7), NIH3T3 (Lane 8), and MCF-7 (Lane 9) cell lysate.
Red: Control Antigen (100 ng)Purple: Antigen (10 ng)Green: Antigen (50 ng)Blue: Antigen (100 ng)
Flow cytometric analysis of HeLa cells using EIF4B antibody Cat.-No AM06769PU-N (green) and Negative Control (red).
Predicted band size: 80 kDa . Positive control: 3T3 and HL60 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: 3T3 and HL60 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human lung carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human lung carcinoma tissue using EIF4B (Ab-422) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
Western blot analysis of extracts of various cell lines, using EIF4B antibody.
Immunohistochemistry of paraffin-embedded human breast cancer using EIF4B antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of U20S cell using EIF4B antibody. Blue: DAPI for nuclear staining.
Host: Rabbit; Target Name: Eif4b; Sample Tissue: Mouse Brain lysates; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-EIF4B Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate; EIF4B is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Rabbit Anti-EIF4B Antibody; Paraffin Embedded Tissue: Human Heart; Cellular Data: Myocardial cells; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
WB Suggested Anti-EIF4B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: ACHN cell lysate; EIF4B is strongly supported by BioGPS gene expression data to be expressed in Human ACHN cells
Western blot analysis of eIF4B expression in HepG2 cells
Western blot analysis of eIF4B (Phospho-Ser422) expression in HepG2 cells
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06769PU-N | EIF4B antibody |
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Mouse | IgG2b | 1F5 | Purified | Hu, Ms | E, ICC/IF, WB |
0.1 mg /
€410.00
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| OriGene Technologies GmbH | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA323172 | EIF4B antibody |
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| Anti-EIF4B Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu | P, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA332671 | EIF4B antibody |
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| Rabbit anti-EIF4B Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA348012 | EIF4B antibody |
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| Rabbit Polyclonal Anti-eIF4B Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA348915 | EIF4B antibody |
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| Rabbit Polyclonal Anti-eIF4B (Phospho-Ser422) Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP01821PU-N | EIF4B pSer422 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP09472PU-N | EIF4B pSer422 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP09472PU-S | EIF4B pSer422 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms | P, WB |
50 µg /
€230.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP09474PU-N | EIF4B antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | P, WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP09474PU-S | EIF4B antibody |
||||||
|
Rabbit | Aff - Purified | Hu | P, WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP11771PU-N | EIF4B antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP11772PU-N | EIF4B antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309955 | EIF4B |
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| Recombinant protein of human eukaryotic translation initiation factor 4B (EIF4B) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205574L2V | Lenti ORF particles, Eif4b (GFP-tagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114), 200 uL, >10^7 TU/mL |
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200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR218693L2V | Lenti ORF particles, Eif4b (GFP-tagged) - Mouse eukaryotic translation initiation factor 4B (Eif4b), 200 uL, >10^7 TU/mL |
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200 µl /
€1,340.00
|
|
| OriGene Technologies, Inc. | ||
| MR205574L1V | Lenti ORF particles, Eif4b (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114), 200 uL, >10^7 TU/mL |
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200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR218693L1V | Lenti ORF particles, Eif4b (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4B (Eif4b), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,200.00
|
|
| OriGene Technologies, Inc. | ||