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EIF5A2 antibody

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Principal name

EIF5A2 antibody

Alternative names for EIF5A2 antibody

eIF-5A-2, eIF-5A2, Eukaryotic translation initiation factor 5A-2, Eukaryotic translation initiation factor 5A-2

SwissProt ID

P23301 (Yeast), Q07460 (Chick), Q20751 (Caeel), Q8BGY2 (Mouse), Q93VP3 (Arath), Q9GZV4 (Human), Q9UST4 (Schpo)

Gene ID

56648 (EIF5A2), 208691 (Eif5a2)

Ncbi ID

9606, NP_065123, NP_065123.1, NM_020390, NP_808254.1, NM_177586

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), GP (Guinea Pig), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Frozen Sections (C)

Background of EIF5A2 antibody

Eukaryotic translation initiation factor 5A2 (eIF5A-2), a recently discovered oncogene member of the eIF5A family, is an mR-binding protein implicated in the translation elongation process and it exerts a critical role at the level of mR turnover, probably acting downstream of decapping. eIF5A-2 involves in actin dymics, progression of cell cycle, mR decay, stress response sigling as well as in the preservation of cellular wall's integrity. eIF5A-2 mediates the effects of polyamines on neurol process extension/survival, and has been suggested to play a role in CNS development/functions as well as differentiation of skeletal muscle stem cells. eIF5A-2 has been reported to localize in nucleus, cytoplasm and ER membranes of cells, and hypusine modification, a posttranslatiol modification of at least one lysine residue to form hypusine [N-epsilon-(4-aminobutyl)lysine], as well as apoptosis impacts eIF5A-2 localization in various cellular compartments. eIF5A-2 exhibits weak expression levels in lungs, testis and brain cells, however, its elevated expression has been shown to be associated with the presence of cancer metastases in colon, ovarian, lung and bladder cancers. In vitro and in vivo studies have revealed that overexpression of eIF5A-2 could significantly enhance the malignt tumor cell motility/invasiveness, whereas, its intracellular depletion leads to inhibition of cell growth as well as induction of apoptosis.

General readings

Guan XY., et al. (2010) Hepatology. 51(4):1255-63.
Luk JM., et al. (2009) Int J Cancer. 127(4):968-76.

H00056648-M01 - EIF5A2
Detection limit for recombinant GST tagged EIF5A2 is approximately 0.1ng/ml as a capture antibody.
H00056648-D01P - EIF5A2
Western Blot analysis of EIF5A2 expression in transfected 293T cell line (H00056648-T02) by EIF5A2 MaxPab polyclonal antibody.Lane 1: EIF5A2 transfected lysate(16.80 KDa).Lane 2: Non-transfected lysate.
H00056648-B01P - EIF5A2
Immunofluorescence of purified MaxPab antibody to EIF5A2 on HeLa cell. [antibody concentration 10 ug/ml]
H00056648-B01P - EIF5A2
Western Blot analysis of EIF5A2 expression in transfected 293T cell line (H00056648-T01) by EIF5A2 MaxPab polyclonal antibody.Lane 1: EIF5A2 transfected lysate(16.83 KDa).Lane 2: Non-transfected lysate.
H00056648-D01 - EIF5A2
Immunoprecipitation of EIF5A2 transfected lysate using anti-EIF5A2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with EIF5A2 purified MaxPab mouse polyclonal antibody (B01P) (H00056648-B01P).
AR09533PU-L - EIF5A2
AR09533PU-L - EIF5A2
Recombinant human EIF5A2, 1-153aa, His-tagged
ARP57402_P050 - EIF5A2
Human Liver; WB Suggested Anti-EIF5A2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Liver; EIF5A2 antibody - N-terminal region (ARP57402_P050) in Human Liver cells using Western Blot
TA505083 - EIF5A2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF5A2 (RC206249, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF5A2.
TA505083 - EIF5A2
HEK293T cells transfected with either RC206249 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EIF5A2 antibody(TA505083), and then analyzed by flow cytometry.
TA505100 - EIF5A2
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EIF5A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505100)
TA505100 - EIF5A2
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF5A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505100)
TA505100 - EIF5A2
Anti-EIF5A2 mouse monoclonal antibody (TA505100) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF5A2(RC206249).
TA505100 - EIF5A2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF5A2 (RC206249, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF5A2.
TA505100 - EIF5A2
HEK293T cells transfected with either RC206249 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EIF5A2 antibody(TA505100), and then analyzed by flow cytometry.
TA505147 - EIF5A2
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EIF5A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505147)
TA505147 - EIF5A2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF5A2 (RC206249, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF5A2.
TA505147 - EIF5A2
HEK293T cells transfected with either RC206249 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EIF5A2 antibody(TA505147), and then analyzed by flow cytometry.
TP306249 - EIF5A2
TA308787 - EIF5A2
Sample(30ug of whole cell lysate). A: 293T. B: A431 . C: H1299. D: HeLa S3 . E: Hep G2 . F: MOLT4 . G: Raji . 15% SDS PAGE. TA308787 diluted at 1:1000
TA308787 - EIF5A2
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EIF5A2 antibody(TA308787) antibody at 1:500 dilution.
TA308787 - EIF5A2
Immunofluorescence analysis of methanol-fixed HeLa, using EIF5A2(TA308787) antibody at 1:200 dilution.
TA336587 - EIF5A2
Western Blot: eIF5A2 Antibody [TA336587] - Detection of endogenous and FLAG-tagged eIF5A1 in HeLa cells using NB 200-133 at a 1:1000 dilution
TA344786 - EIF5A2
WB Suggested Anti-EIF5A2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Liver
  1. Quick-Filters:
  2. Highly Validated (1)

7 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF5A2 antibody
  EIF5A2 mouse monoclonal antibody, clone OTI6G7 (formerly 6G7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EIF5A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505100) Mouse IgG1 OTI6G7 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+4 additional images

Highly validated antibody!

TrueMAB

EIF5A2 antibody
  Rabbit Polyclonal antibody to EIF5A2 (eukaryotic translation initiation factor 5A2)  
Sample(30ug of whole cell lysate). A: 293T. B: A431 . C: H1299. D: HeLa S3 . E: Hep G2 . F: MOLT4 . G: Raji . 15% SDS PAGE. TA308787 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

EIF5A2 antibody
  Rabbit Polyclonal eIF5A2 Antibody  
Western Blot: eIF5A2 Antibody [TA336587] - Detection of endogenous and FLAG-tagged eIF5A1 in HeLa cells using NB 200-133 at a 1:1000 dilution Rabbit Purified Hu C, E, IP, P, WB
0.1 mg / €395.00
  OriGene Technologies, Inc.

EIF5A2 antibody
  EIF5A2 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF5A2 (RC206249, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF5A2. Mouse IgG1 OTI2E9 Purified Hu F, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

EIF5A2 antibody
  EIF5A2 mouse monoclonal antibody, clone OTI6F7 (formerly 6F7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EIF5A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505147) Mouse IgG1 OTI6F7 Purified Hu F, P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+2 additional images

TrueMAB

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EIF5A2
  Recombinant protein of human eukaryotic translation initiation factor 5A2 (EIF5A2)  
EIF5A2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

EIF5A2 overexpression lysate
EIF5A2 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

7 Item(s)

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Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (5)
  3. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (3)
  2. Polyclonal (2)
Target
  1. EIF5A2 (6)
Reactivity
  1. Human (Hu) (5)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (4)
  3. Flow cytometry (F) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Frozen sections (C) (1)
  6. Enzyme Immunoassay (E) (1)
  7. Immunoprecipitation (IP) (1)
Presentation
  1. Purified (4)
  2. Aff - Purified (1)
Host
  1. Mouse (3)
  2. Rabbit (2)
Images
  1. 1 image (6)
  2. 3+ images (3)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Flow cytometry (F) (3)
  3. Paraffin Sections (P) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  5. Immunofluorescence (IF) (1)
  6. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (7)
Service
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