ELF1 antibody
Principal name
ELF1 antibody
Alternative names for ELF1 antibody
Elf-1, ETS-related transcription factor Elf-1, E74-like factor 1
Ncbi ID
9606, NP_031946, NP_758961, NP_758961.1, NP_031946.1, NM_172373, NM_001145353
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of ELF1 antibody
- Kit Component:
- - KN305146G1, Elf1 gRNA vector 1 in pCas-Guide vector
- KN305146G2, Elf1 gRNA vector 2 in pCas-Guide vector
- KN305146D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Wang CY, Petryniak B, Thompson CB, Kaelin WG, Leiden JM.
Regulation of the Ets-related transcription factor Elf-1 by binding to the retinoblastoma protein.
Science. 1993 May 28;260(5112):1330-5.
PMID: 8493578
ELF1 monoclonal antibody (M01), clone 3B7 Western Blot analysis of ELF1 expression in 293 ( Cat # L026V1 ).
Detection limit for recombinant GST tagged ELF1 is approximately 1ng/ml as a capture antibody.
Mouse SP2/0; WB Suggested Anti-ELF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: SP2/0 cell lysate; ELF1 antibody - N-terminal region (ARP36845_P050) in Mouse SP2/0 cells using Western Blot
ELF1 polyclonal antibody ( Cat # PAB6207 ) staining ( 0.3 µg/ml ) of human tonsil lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot analysis of ELF1 expression in transfected 293T cell line (H00001997-T01) by ELF1 MaxPab polyclonal antibody.Lane 1: ELF1 transfected lysate(68.09 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ELF1 expression in transfected 293T cell line (H00001997-T01) by ELF1 MaxPab polyclonal antibody.Lane 1: ELF1 transfected lysate(68.09 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ELF1 expression in transfected 293T cell line (H00001997-T01) by ELF1 MaxPab polyclonal antibody.Lane 1: ELF1 transfected lysate(67.50 KDa).Lane 2: Non-transfected lysate.
Human Spleen; Spleen; ELF1 antibody - N-terminal region (ARP31085_P050) in Human Spleen cells using Immunohistochemistry
Human HeLa; WB Suggested Anti-ELF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate; ELF1 antibody - N-terminal region (ARP31085_P050) in Human HeLa cells using Western Blot
Human 293T; WB Suggested Anti-ELF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Transfected 293T; ELF1 antibody - middle region (P100872_P050) in Human 293T cells using Western Blot
TA302810 staining (0.3µg/ml) of Human Tonsil lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of extracts from 293 cells and HuvEc cells, using ELF1 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using ELF1 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-ELF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Transfected 293T
WB Suggested Anti-ELF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: SP2/0 cell lysate
Western blot analysis on 293 cell lysate using ELF1 Antibody
WB Suggested Anti-ELF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Hela cell lysate
Spleen
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302810 | ELF1 (C-term) antibody |
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| Goat Polyclonal Antibody against ELF1 | |||||||
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Goat | Aff - Purified | Hu, Ms | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA313807 | ELF1 antibody |
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| Rabbit polyclonal anti-ELF1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA347618 | ELF1 antibody |
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| Rabbit Polyclonal Anti-ELF1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760629 | ELF1 (full length, N-term HIS tag, transcript variant 1) |
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| Purified recombinant protein of Human E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
