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EMX1 antibody

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Principal name

EMX1 antibody

Alternative names for EMX1 antibody

Empty spiracles homolog 1, Empty spiracles-like protein 1, Homeobox protein EMX1

SwissProt ID

Q04741 (Human), Q04742 (Mouse)

Gene ID

2016 (EMX1), 13796 (Emx1)

Ncbi ID

NP_004088, NM_004097, NP_004088.2, NP_034261.1, NM_010131

Available reactivities

Can (Canine), Dros (Drosophila), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Chicken

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of EMX1 antibody

Kit Component:
- KN305211G1, Emx1 gRNA vector 1 in pCas-Guide vector
- KN305211G2, Emx1 gRNA vector 2 in pCas-Guide vector
- KN305211D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Bishop KM, Garel S, Nakagawa Y, et al. Emx1 and Emx2 cooperate to regulate cortical size, lamination, neuronal differentiation, development of cortical efferents, and thalamocortical pathfinding. J. Comp. Neurol. 2003; 457:345-60.
Lichtneckert R, Nobs L, Reichert H. Empty spiracles is required for the development of olfactory projection neuron circuitry in Drosophila. Development 2008; 135:2415-24
Gulisano M, et al. Emx1 and Emx2 show different patterns of expression during proliferation and differentiation of the developing cerebral cortex in the mouse. Eur. J. Neurosci. 1996; 8:1037-50.
Chan CH, Godinho LN, Thomidou D, et al. 2001. Emx1 is a marker for pyramidal neurons of the cerebral cortex. Cereb. Cortex 11:1191-8.

ARP47535_P050 - EMX1
Human HepG2; WB Suggested Anti-EMX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; EMX1 antibody - middle region (ARP47535_P050) in Human HepG2 cells using Western Blot
ARP47536_P050 - EMX1
Human HepG2; WB Suggested Anti-EMX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; EMX1 antibody - middle region (ARP47536_P050) in Human HepG2 cells using Western Blot
AP55602CP-N - EMX1

Western blot analysis in rat liver tissue lysate with EMX1 antibody Cat.-No. AP55602PU-N at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.


TA313817 - EMX1
Western blot analysis of extracts from HuvEc and K562 cells, using EMX1 antibody.The lane on the right is treated with the synthesized peptide.
TA319638 - EMX1
Western blot analysis of EMX1 in rat liver tissue lysate with EMX1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
TA325087 - EMX1
EMX1 Antibody (C-term) (Cat. #TA325087) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow).
TA325087 - EMX1
IF image of Hela cell stained with EMX1 Antibody (C-term)(Cat#TA325087).Hela cells were incubated with EMX1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . EMX1 immunoreactivity is localized to Nucleus significantly.
TA325087 - EMX1
EMX1 Antibody (C-term) (Cat. #TA325087) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA342410 - EMX1
WB Suggested Anti-EMX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate
TA342411 - EMX1
WB Suggested Anti-EMX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EMX1 antibody
  Rabbit polyclonal anti-EMX1 antibody  
Western blot analysis of extracts from HuvEc and K562 cells, using EMX1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

EMX1 (N-term) antibody
  Chicken Polyclonal EMX1 Antibody  
Western blot analysis of EMX1 in rat liver tissue lysate with EMX1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Chicken Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.

EMX1 antibody
  Rabbit polyclonal EMX1 Antibody (C-term)  
EMX1 Antibody (C-term) (Cat. #TA325087) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow). Rabbit IgG Purified Hu, Ms F, ICC/IF, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

Lysates

Catalog No.    

EMX1 overexpression lysate
EMX1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

4 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (3)
Clonality
  1. Polyclonal (3)
Target
  1. EMX1 (3)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (2)
  3. Flow cytometry (F) (1)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (2)
  2. Chicken (1)
Images
  1. 1 image (3)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunofluorescence (IF) (1)
Manufacturer
  1. OriGene Technologies (4)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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