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Endomucin antibody

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Principal name

Endomucin antibody

Alternative names for Endomucin antibody

EMCN, EMCN2, MUC14, Endomucin-2, Mucin-14, Gastric cancer antigen Ga34

SwissProt ID

Q6AY82 (Rat), Q9R0H2 (Mouse), Q9ULC0 (Human)

Gene ID

51705 (EMCN), 59308 (Emcn), 295490

Ncbi ID

NP_001153166.1, NP_001156994.1, NP_057326.2, NM_001159694, NM_001163522, NM_016885, NM_001004228, NM_016242

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F)

Background of Endomucin antibody

As a typical mucin-like glycoprotein, endomucin has a high content of serine and threonine residues, suggesting strong O-glycosylation; the sensitivity to O-sialoglycoprotein endopeptidase indicates that endomucin is also a sialomucin.
Endomucin is an endothelial-specific sialomucin. It is a constitutively expressed endothelial cell surface protein that is found on all venules but is absent from high endothelial venule cells (HEV) of peripheral and mesenteric lymph nodes as well as Peyer's patches, the specialized site for most efficient lymphocyte trafficking. This could indicate an anti-adhesive function of endomucin, as demonstrated for other sialomucins.
Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is another cell adhesion molecule that contains a mucin-like domain and is expressed on HEV in Peyer's patches, mesenteric lymph nodes and on venules in intestinal lamina propria. In the HEV of mesenteric lymph nodes, the mucin-like domain of a subpopulation of MAdCAM-1 molecules contains sulfated carbohydrate side chains that interact with L-selectin.
The presence of three putative protein kinase C phosphorylation sites in the cytoplasmic tail of endomucin indicates that endomucin has the capacity to be a signaling molecule.

General readings

Kinoshita M, et al. (2001) FEBS Lett 499 (1-2): 121-6.
Kuhn A., et al. (2002) J. Invest. Dermatol. 119:1388-1393

C11879-1 - Endomucin
Immunofluorescence analysis of HepG2 cells, using Mucin-14 Antibody. The picture on the right is treated with the synthesized peptide.
AR50456PU-N - Endomucin
AR60010PU-N - Endomucin
SDS-PAGE analysis of recombinant mouse soluble Endomucin produced in E. coli Cat.-No. AR60010PU-N. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver staining.
AP60010PU-N - Endomucin
Western analysis using anti-mouse Endomucin antibody Cat.-No. AP60010PU. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa);
Lane 2: rm sEndomucin (250ng).
AP60010PU-N - Endomucin
FACS analysis with primary mouse endothelial cells (SnoMec).
AP60011PU-N - Endomucin
Western blot analysis using anti-Human Endomucin antibody Cat.-No AP60011PU. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa);
Lane 2: rh sEndomucin.
AP60011PU-N - Endomucin
Immunofluorescence staining (green) of cryo-sections of Human foreskin (fixed 15 min in 4% PFA) with anti-Human Endomucin antibody Cat.-No AP60011PU (5 μg/ml) and counter staining of nuclei with DAPI (blue) and double staining wit anti-CD31 (right panel).
Performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.
AP60010PU-S - Endomucin
Immunofluorescence staining (green) of cryo-sections of adult mouse kidney (fixed 15 min in 4% PFA) with anti-mouse Endomucin antibody Cat.-No. AP60010PU-S (5μg/ml) and counter staining of nuclei with DAPI (blue). Performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.
AP60010PU-S - Endomucin
Western analysis using anti-mouse Endomucin antibody Cat.-No. AP60010PU-N. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa).
Lane 2: rm sEndomucin (250ng).
AP60010PU-S - Endomucin
FACS analysis with primary mouse endothelial cells (SnoMec).
AR31181PU-N - Endomucin
SDS-PAGE analysis of recombinant human soluble Endomucin produced in E. coli. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
TA308287 - Endomucin
Sample (30 ug of whole cell lysate). A: A431 . 12% SDS PAGE. TA308287 diluted at 1:1000.
TA308287 - Endomucin
Endomucin antibody [N1C2] detects Endomucin protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Endomucin antibody [N1C2] (TA308287) dilution: 1:250.
TA312706 - Endomucin
Immunofluorescence analysis of HepG2 cells, using Mucin-14 antibody.The picture on the right is treated with the synthesized peptide.
TA338051 - Endomucin
Host: Rabbit; Target Name: EMCN; Sample Tissue: 293T Whole Cell lysates; Antibody Dilution: 1.0 ug/ml

13 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Endomucin antibody
  Rabbit polyclonal antibody to Endomucin (endomucin)  
Sample (30 ug of whole cell lysate). A: A431 . 12% SDS PAGE. TA308287 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

Endomucin antibody
  Rabbit polyclonal anti-Mucin-14 antibody  
Immunofluorescence analysis of HepG2 cells, using Mucin-14 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF
0.1 ml / €335.00
  OriGene Technologies, Inc.

Endomucin antibody
Endomucin Rat IgG2a V.7C7.1 Purified Ms C, IP, P, WB
0.1 mg / €320.00
  OriGene Technologies GmbH

Endomucin antibody
Western analysis using anti-mouse Endomucin antibody Cat.-No. AP60010PU. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rm sEndomucin (250ng). Rabbit IgG Purified Ms F, WB
0.2 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

Endomucin antibody
Western analysis using anti-mouse Endomucin antibody Cat.-No. AP60010PU. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rm sEndomucin (250ng). Rabbit IgG Purified Ms F, WB
0.1 mg / €270.00
  OriGene Technologies GmbH
+1 additional image

Endomucin antibody
Immunofluorescence staining (green) of cryo-sections of adult mouse kidney (fixed 15 min in 4% PFA) with anti-mouse Endomucin antibody Cat.-No. AP60010PU-S (5μg/ml) and counter staining of nuclei with DAPI (blue). <i>Performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.</i> Rabbit IgG Aff - Purified Ms C, ICC/IF, WB
50 µg / €280.00
  OriGene Technologies GmbH
+2 additional images

Endomucin antibody
Western blot analysis using anti-Human Endomucin antibody Cat.-No AP60011PU. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rh sEndomucin. Rabbit IgG Purified Hu C, ICC/IF, WB
0.2 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

Endomucin antibody
Western blot analysis using anti-Human Endomucin antibody Cat.-No AP60011PU. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rh sEndomucin. Rabbit IgG Purified Hu C, ICC/IF, WB
0.1 mg / €270.00
  OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.    

EMCN overexpression lysate
EMCN overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Emcn (GFP-tagged) - Mouse endomucin (Emcn), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Emcn (GFP-tagged) - Mouse endomucin (Emcn), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Emcn (Myc-DDK-tagged) - Mouse endomucin (Emcn), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Emcn (Myc-DDK-tagged) - Mouse endomucin (Emcn), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Emcn (GFP-tagged ORF) - Rat endomucin (Emcn), 200 uL, >10^7 TU/mL
Lenti ORF particles, Emcn (GFP-tagged ORF) - Rat endomucin (Emcn), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Emcn (Myc-DDK-tagged ORF) - Rat endomucin (Emcn), 200 uL, >10^7 TU/mL
Lenti ORF particles, Emcn (Myc-DDK-tagged ORF) - Rat endomucin (Emcn), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

13 Item(s)

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13 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (8)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (7)
  2. Monoclonal (1)
Target
  1. Endomucin (8)
Reactivity
  1. Mouse (Ms) (5)
  2. Human (Hu) (4)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Frozen sections (C) (4)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (4)
  4. Flow cytometry (F) (2)
  5. Enzyme Immunoassay (E) (1)
  6. Immunoprecipitation (IP) (1)
  7. Paraffin Sections (P) (1)
Presentation
  1. Purified (6)
  2. Aff - Purified (2)
Host
  1. Rabbit (7)
  2. Rat (1)
Images
  1. 1 image (7)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Flow cytometry (F) (3)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  4. Immunofluorescence (IF) (1)
  5. IHC (1)
Manufacturer
  1. OriGene Technologies (13)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
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  • fax: +49-5221-34606-11
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