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EPHA4 antibody

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Principal name

EPHA4 antibody

Alternative names for EPHA4 antibody

HEK8, SEK, TYRO1, Ephrin type-A receptor 4, Tyrosine-protein kinase receptor SEK, Receptor protein-tyrosine kinase HEK8, Tyrosine-protein kinase TYRO1

SwissProt ID

P54764 (Human), Q03137 (Mouse), Q07496 (Chick)

Gene ID

2043 (EPHA4), 13838 (Epha4)

Ncbi ID

NP_004429, 9606, NP_004429.1, no protein_acc, NM_004438, BC004782, NM_007936, NP_031962.2

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Bt (Bat), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Tr (Turkey), L (Lizard), Bear, Xen (Xenopus)

Available hosts

Goat, Mouse, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of EPHA4 antibody

Eph receptor A4 is a receptor for members of the ephrin-A family. It binds to ephrin-A1, -A4 and -A5 but binds more poorly to ephrin-A2 and -A3. Eph receptor A4 may play a role in a signal transduction process involved in hindbrain pattern formation.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

H00002043-M02 - EPHA4
EPHA4 monoclonal antibody (M02), clone 6H7 Western Blot analysis of EPHA4 expression in NIH/3T3 ( Cat # L018V1 ).
H00002043-M02 - EPHA4
Detection limit for recombinant GST tagged EPHA4 is approximately 0.1ng/ml as a capture antibody.
AP00371PU-N - EPHA4
Immunohistochemistry Image: Glomerulus, stained with AP00371PU-N
PAB3007 - EPHA4
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA4 polyclonal antibody ( Cat # PAB3007 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB3007 - EPHA4
Methanol/Acetone fixed human stem cell is used in IF to detect EPHA4 ( blue ) and endothelial Lectin ( red ) . Data kindly provided by Dr. Weis from Cheresh Lab, UCSD.
PAB3007 - EPHA4
Western blot analysis of EPHA4 polyclonal antibody ( Cat # PAB3007 ) in HeLa cell lysate. EPHA4 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AP14278PU-N - EPHA4
TOP: Methanol/Acetone fixed human stem cell is used in IF to detect Eph4A (blue) and endothelial Lectin(red). Data kindly provided by Dr. Weis from Cheresh Lab, UCSD. BOTTOM:Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14278PU-N - EPHA4
(LEFT)Western blot analysis of anti-EphA4 N-term Pab in HeLa cell lysate. EphA4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of EphA4 (arrow) using EphA4 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA4 gene (Lane 2)
AP14279PU-N - EPHA4
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14279PU-N - EPHA4
(LEFT)Western blot analysis of anti-EphA4 Pab in NCI-H460 cell lysate. EphA4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of EPHA4 (arrow) using EphA4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2)
AM06240SU-N - EPHA4
Western blot analysis using EphA4 mouse mAb against truncated Trx-EphA4 recombinant protein (1) and truncated GST-EphA4(aa777-986) recombinant protein (2).
AM06261SU-N - EPHA4
Western blot analysis using EphA3 antibody Cat.-No AM06261SU-N against truncated Trx-EphA3 recombinant protein (Lane 1) and truncated EphA3 (aa566-983)-hIgGFc transfected CHO-K1 cell lysate (Lane 2).
AP55848PU-S - EPHA4
Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody AP55848PU-N. The lane on the left is treated with antigen-specific peptide.
TP700141 - EPHA4
TA322742 - EPHA4
Predicted cell location: Cell membrane, Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using EPHA4 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA341077 - EPHA4
Anti-EPHA4 antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA341082 - EPHA4
Anti-EPHA4 antibody IHC of human glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA348043 - EPHA4
Western blot analysis of EPHA4 expression in JurKat cells

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EPHA4 antibody
  Anti-EPHA4 Rabbit Polyclonal Antibody  
Predicted cell location: Cell membrane, Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using EPHA4 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

EPHA4 antibody
  Rabbit Polyclonal Anti-EPHA4 Antibody (Internal)  
EPHA4 Rabbit Purified Hu
50 µg / €425.00
  OriGene Technologies, Inc.

EPHA4 antibody
  Rabbit Polyclonal Anti-EPHA4 Antibody (Internal)  
Anti-EPHA4 antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hst, Hu, Ms, Rb, Rt, Xen
50 µg / €425.00
  OriGene Technologies, Inc.

EPHA4 (Internal) antibody
  Rabbit Polyclonal Anti-EPHA4 Antibody (Internal)  
EPHA4 Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, L, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Xen P
50 µg / €425.00
  OriGene Technologies, Inc.

EPHA4 antibody
  Rabbit Polyclonal Anti-EPHA4 Antibody  
Western blot analysis of EPHA4 expression in JurKat cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

EPHA4 antibody
Western blot analysis using EphA4 mouse mAb against truncated Trx-EphA4 recombinant protein (1) and truncated GST-EphA4(aa777-986) recombinant protein (2). Mouse IgG1 7D3D4 Ascites Hu WB
0.1 ml / €340.00
  OriGene Technologies GmbH

EPHA4 antibody
Western blot analysis using EphA3 antibody Cat.-No AM06261SU-N against truncated Trx-EphA3 recombinant protein (Lane 1) and truncated EphA3 (aa566-983)-hIgGFc transfected CHO-K1 cell lysate (Lane 2). Mouse IgG2b 6C1B6 Ascites Hu WB
0.1 ml / €380.00
  OriGene Technologies GmbH

EPHA4 (C-term) antibody
Immunohistochemistry Image: Glomerulus, stained with AP00371PU-N Rabbit Aff - Purified Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Rb, Rt E, P
50 µg / €425.00
  OriGene Technologies GmbH

EPHA4 (N-term) antibody
TOP: Methanol/Acetone fixed human stem cell is used in IF to detect Eph4A (blue) and endothelial Lectin(red). Data kindly provided by Dr. Weis from Cheresh Lab, UCSD. BOTTOM:Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu ICC/IF, P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

EPHA4 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

EPHA4 pTyr596 antibody
Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody AP55848PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €300.00
  OriGene Technologies GmbH

EPHA4 pTyr596 antibody
Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody AP55848PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µg / €230.00
  OriGene Technologies GmbH

EPHA4 antibody
EphA4 staining of cartillage primordium of a rib (crossection) in rat embryos at E15 developmental stage. Cells were stained using anti-goat Cy3 (red).   Goat Aff - Purified Ms, Rt C, E, WB
0.1 mg / €450.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

EPHA4 - human gene knockout kit via CRISPR
EPHA4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EPHA4 - human gene knockout kit via CRISPR
EPHA4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EPHA4 - human gene knockout kit via CRISPR
EPHA4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EPHA4 - human gene knockout kit via CRISPR
EPHA4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EPHA4 (C-term DDK/His tag)
  Purified recombinant protein of Human EPH receptor A4 (EPHA4), with C-terminal DDK/His tag, expressed in human cells  
EPHA4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Epha4 (GFP-tagged) - Mouse Eph receptor A4 (cDNA clone MGC:8084 IMAGE:3588285), 200 uL, >10^7 TU/mL
Lenti ORF particles, Epha4 (GFP-tagged) - Mouse Eph receptor A4 (cDNA clone MGC:8084 IMAGE:3588285), 200 uL, >10^7 TU/mL  
200 µl / €1,470.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Epha4 (GFP-tagged) - Mouse Eph receptor A4 (Epha4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Epha4 (GFP-tagged) - Mouse Eph receptor A4 (Epha4), 200 uL, >10^7 TU/mL  
200 µl / €1,870.00
  OriGene Technologies, Inc.
 

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22 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (4)
  2. Mouse Lenti ORF Particle (4)
  3. Primary Antibody (13)
  4. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (11)
  2. Monoclonal (2)
Target
  1. EPHA4 (14)
Property
  1. C-term (2)
  2. Internal (1)
  3. N-term (1)
  4. pTyr596 (2)
Reactivity
  1. Human (Hu) (12)
  2. Mouse (Ms) (8)
  3. Rat (Rt) (6)
  4. Hamster (Hst) (3)
  5. Rabbit (Rb) (3)
  6. Bovine (Bov) (2)
  7. Bat (Bt) (2)
  8. Canine (Can) (2)
  9. Elephant (2)
  10. Equine (Eq) (2)
  11. Monkey (Mky) (2)
  12. Xenopus (Xen) (2)
  13. Bear (1)
  14. Chicken (Chk) (1)
  15. Lizard (L) (1)
  16. Opossum (Op) (1)
  17. Platypus (1)
  18. Porcine (Por) (1)
  19. Primate (Prim) (1)
  20. Turkey (Tr) (1)
Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Paraffin Sections (P) (5)
  3. Enzyme Immunoassay (E) (3)
  4. Frozen sections (C) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (6)
  2. Aff - Purified (5)
  3. Ascites (2)
Host
  1. Rabbit (10)
  2. Mouse (2)
  3. Goat (1)
Images
  1. 1 image (12)
Images: Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (3)
  3. IHC (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (21)
  2. Neuromics Antibodies (1)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
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