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EPHA5 antibody

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Principal name

EPHA5 antibody

Alternative names for EPHA5 antibody

EHK1, HEK7, Ephrin type-A receptor 5, Tyrosine-protein kinase receptor EHK-1, EPH homology kinase 1, Receptor protein-tyrosine kinase HEK7

SwissProt ID

P54755 (Chick), P54756 (Human), P54757 (Rat), Q60629 (Mouse)

Gene ID

2044 (EPHA5)

Ncbi ID

NP_004430.4, 10090, 9606, NP_004430, NM_004439.3, NP_004430.3, NM_004439, NM_182472

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of EPHA5 antibody

EPHA5 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

General readings

Mehrle,A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006)

PAB3009 - EPHA5
Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 polyclonal antibody ( Cat # PAB3009 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PAB3009 - EPHA5
Western blot analysis of EPHA5 polyclonal antibody ( Cat # PAB3009 ) in mouse brain tissue. EPHA5 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AM11061PU-N - EPHA5
Figure 2. Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody (Cat.#AM11061PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AM11061PU-N - EPHA5
Figure 1. Western blot analysis of anti-EPHA5 Monoclonal Antibody (Cat.#AM11061PU-N) by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified Mab.(1:2000).
AP14281PU-N - EPHA5
Formalin-fixed and paraffin-embedded human brain tissue reacted with EphA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14281PU-N - EPHA5
Western blot analysis of anti-EphA5 N-term Pab in mouse brain tissue. EphA5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AP14282PU-N - EPHA5
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
AP14282PU-N - EPHA5
(LEFT) Western blot analysis of anti-EphA5 C-term Pab in mouse brain tissue. EphA5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT) Western blot analysis of anti-EphA5 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). EPHA5(arrow) was detected using the purified Pab.
AP14283PU-N - EPHA5
Western blot analysis of EphA5 (arrow) using rabbit polyclonal EphA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA5 gene (Lane 2)
ARP58460_P050 - EPHA5
Human Brain; WB Suggested Anti-EPHA5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; EPHA5 antibody - middle region (ARP58460_P050) in Human Brain cells using Western Blot
AM06295SU-N - EPHA5
Western blot analysis using EPHA5 mouse mAb against truncated EPHA5-His recombinant protein (1) and truncated EPHA5(aa620-774)-hIgGFc transfected CHO-K1 cell lysate(2).
CPA1392-100ul - EPHA5
Western blot analysis of EPHA5 expression in SKNSH (A); A549 (B) whole cell lysates.
TP700142 - EPHA5
TP710130 - EPHA5
TA313828 - EPHA5
Western blot analysis of extracts from HepG2 cells and A549 cells, using EPHA5 antibody.The lane on the right is treated with the synthesized peptide.
TA344332 - EPHA5
WB Suggested Anti-EPHA5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human brain
TA347926 - EPHA5
Western blot analysis of EPHA5 expression in HeLa cell lysate

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EPHA5 antibody
  Rabbit polyclonal anti-EPHA5 antibody  
Western blot analysis of extracts from HepG2 cells and A549 cells, using EPHA5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

EPHA5 antibody
  Rabbit Polyclonal Anti-EPHA5 Antibody  
Western blot analysis of EPHA5 expression in HeLa cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

EPHA5 antibody
Western blot analysis using EPHA5 mouse mAb against truncated EPHA5-His recombinant protein (1) and truncated EPHA5(aa620-774)-hIgGFc transfected CHO-K1 cell lysate(2). Mouse IgG1 8B10B1 Ascites Hu E, WB
0.1 ml / €340.00
  OriGene Technologies GmbH

EPHA5 antibody
Figure 2. Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody (Cat.#AM11061PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Mouse IgG1 46CT61.6.4 Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

EPHA5 (N-term) antibody
Formalin-fixed and paraffin-embedded human brain tissue reacted with EphA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

EPHA5 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

EPHA5 antibody
Western blot analysis of EphA5 (arrow) using rabbit polyclonal EphA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA5 gene (Lane 2) Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EPHA5 (C-term DDK/His tag, transcript variant 1)
  Purified recombinant protein of human EPH receptor A5 (EPHA5), transcript variant 1, with C-terminal DDK/His tag, expressed in human cells, 20 µg  
EPHA5 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

EPHA5 (C-term DDK tag, transcript variant 1)
  Recombinant protein of human EPH receptor A5 (EPHA5), transcript variant 1, residues 25-573aa, with C-terminal DDK tag, expressed in sf9, 20ug  
EPHA5 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 SF9 cells
20 µg / €411.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

EPHA5 overexpression lysate
EPHA5 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

10 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (7)
  3. Protein | Growth Factor (2)
Clonality
  1. Polyclonal (5)
  2. Monoclonal (2)
Target
  1. EPHA5 (9)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (3)
  3. Enzyme Immunoassay (E) (2)
Presentation
  1. Purified (6)
  2. Ascites (1)
Host
  1. Rabbit (5)
  2. Mouse (2)
Images
  1. 1 image (9)
Images: Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (3)
Species (Protein)
  1. Human (2)
Source
  1. HEK293 cells (1)
  2. SF9 cells (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
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