EPHA7 antibody
Principal name
EPHA7 antibody
Alternative names for EPHA7 antibody
EHK3, HEK11, Ephrin type-A receptor 7, Tyrosine-protein kinase receptor EHK-3, EPH homology kinase 3, Receptor protein-tyrosine kinase HEK11
Ncbi ID
9606, NP_004431.1, NP_004431, NM_004440, 10090, BC026153, NM_010141, NM_001122889, NM_134331
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)
Background of EPHA7 antibody
The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date. The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1 (Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8(Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3 (Cek10, Hek2), EphB4 (Htk), EphB5(Hek9) and EphB6 (Mep). EphAs are a family of receptor tyrosine kinases that are involved in axonal guidance during development. These receptors and their ligands, the ephrins, act via repulsive mechanisms to guide growing axons towards their appropriate targets and allow for the correct developmental connections to be made. Ligand binding to an Eph receptor results in tyrosine phosphorylation of the kinase domain, and repulsion of axonal growth cones and migrating cells. During neurulation, ephrin-A5 is coexpressed with its cognate receptor EphA7 in cells at the edges of the dorsal neural folds. Three different EphA7 splice variants, a full-length form and two truncated versions lacking kinase domains, are expressed in the neural folds.
Western Blot analysis of EPHA7 expression in transfected 293T cell line (H00002045-T01) by EPHA7 MaxPab polyclonal antibody.Lane 1: EPHA7 transfected lysate(30.69 KDa).Lane 2: Non-transfected lysate.
Western blot (WB) analysis of EphA7 antibody (Cat.-No.: AP06735PU-N) in extracts from Jurkat cells.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
(LEFT)Western blot analysis of anti-EphA7 Pab in NCI-H460 cell lysate. EphA7 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of EPHA7 (arrow) using EphA7 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA7 gene (Lane 2)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EphA7 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of EphA7 (arrow) using rabbit polyclonal EphA7 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA7 gene (Lane 2)
Immunoprecipitation of EPHA7 transfected lysate using anti-EPHA7 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with EPHA7 MaxPab mouse polyclonal antibody (B01) (H00002045-B01).
Immunohistochemical analysis of paraffin-embedded Human Breast tissues using EPHA7 antibody Cat.-No AM06281SU-N
Western blot analysis using EphA7 antibody Cat.-No AM06281SU-N against truncated GST-EphA7 recombinant protein (lane 1) and truncated EphA7 (aa25-556)-hIgGFc transfected CHOK1 cell lysate (Lane 2).
Western blot analysis of extracts from JK cells (Lane 2), using EPHA7 (Phospho-Tyr791) Antibody AP55849PU-N. The lane on the left is treated with antigen-specific peptide.
Western blot analysis of EPHA7 expression in HEK293T (A); Jurkat (B) whole cell lysates.
Western blot analysis of extracts from JK cells, using EPHA7 (Ab-791)antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using EPHA7 (Ab-791) Antibody. The lane on the left is treated with synthesized peptide.
Western blot analysis of extracts from Jurkat cells and COLO205 cells, using EPHA7 antibody.The lane on the right is treated with the synthesized peptide.
Host:Rabbit; Target Name:EPHA7; Sample Tissue:Human Fetal Liver; Antibody Dilution: 1.0 ug/ml
Western blot analysis on JK cell lysate using EPHA7 Antibody
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (EPHA7 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313829 | EPHA7 antibody |
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| Rabbit polyclonal EPHA7 (Ab-791) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA313831 | EPHA7 antibody |
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| Rabbit polyclonal anti-EPHA7 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347705 | EPHA7 antibody |
||||||
| Rabbit Polyclonal Anti-EPHA7 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350913 | EPHA7 antibody |
||||||
| Rabbit Polyclonal Anti-EPHA7 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM06281SU-N | EPHA7 antibody |
||||||
 |
Mouse | IgG2b | 6C8G7 | Ascites | Hu | P, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP06735PU-N | EPHA7 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP14288PU-N | EPHA7 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP14289PU-N | EPHA7 antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP55849PU-N | EPHA7 pTyr791 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| AP55849PU-S | EPHA7 pTyr791 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP326293 | EPHA7 |
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| Recombinant protein of human EPH receptor A7 (EPHA7) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
| TP700144 | EPHA7 (C-term DDK/His tag) |
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| Purified recombinant protein of human EPH receptor A7 (EPHA7), with C-terminal DDK/His tag, expressed in human cells, 20 µg | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP710132 | EPHA7 (C-term DDK tag) |
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| Recombinant protein of human EPH receptor A7 (EPHA7), residues 28-555aa, with C-terminal DDK tag, expressed in sf9, 20ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211406L2V | Lenti ORF particles, Epha7 (GFP-tagged) - Mouse Eph receptor A7 (cDNA clone MGC:14056 IMAGE:3991628), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,470.00
|
|
| OriGene Technologies, Inc. | ||
| MR211406L1V | Lenti ORF particles, Epha7 (Myc-DDK-tagged) - Mouse Eph receptor A7 (cDNA clone MGC:14056 IMAGE:3991628), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,330.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212203L2V | Lenti ORF particles, Epha7 (GFP-tagged ORF) - Rat Eph receptor A7 (Epha7), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,890.00
|
|
| OriGene Technologies, Inc. | ||
| RR212203L1V | Lenti ORF particles, Epha7 (Myc-DDK-tagged ORF) - Rat Eph receptor A7 (Epha7), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,700.00
|
|
| OriGene Technologies, Inc. | ||
