Ephrin-B1 antibody
Principal name
Ephrin-B1 antibody
Alternative names for Ephrin-B1 antibody
EFL-3, EFNB1, EFL3, EPLG2, LERK2, ELK-L, LERK-2, CEK5-L, EPH-related receptor tyrosine kinase ligand 2, CEK5 LIgand
Ncbi ID
9606, NP_004420, NP_990366, NP_001080948, NP_004420.1, NM_004429, NP_034240.1, NM_010110
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of Ephrin-B1 antibody
Ephrin-B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). Ephrin-B molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin-B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin-B is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific Ephrin-B residue (Tyr298) plays a key role in Ephrin-B signaling (Kalo, et al., 2001).
General readings
Nagashima K., et al. (2002) Mol Biol Cell. 13:4231-4242.
Western blot analysis of Ephrin-B antibody (Cat.-No. AP06387PU-N) in extracts from 293 cells treated with EGF 200ng/ml 5' or 293 cells treated with TNF-a 20ng/ml 30'.
Western blot of rat testis lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr317 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase, lane 2). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the EphrinB (phospho Y317) polyclonal antibody ( Cat # PAB9611 ). The immunolabeling of the EphrinB is completely eliminated by treatment with l-Ptase.
Western blot of rat testis lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr331 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the EphrinB (phospho Y331) polyclonal antibody ( Cat # PAB9612 ). The immunolabeling is completely eliminated by treatment with l-Ptase.
Western blot of rat testis lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr298 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase, lane 2). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the EphrinB (phospho Y298) polyclonal antibody ( Cat # PAB9690 ). The immunolabeling of the EphrinB band is completely eliminated by treatment with l-Ptase.
Figure 1. Western blot of rat testes lysate showing specific immunolabeling of the ~46k Ephrin-B phosphorylated at Tyr298 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr298 Ephrin-B. The immunolabeling of the Ephrin-B band is completely eliminated by treatment with lambda-Ptase.
Figure 1. Western blot of rat testes lysate showing specific immunolabeling of the ~46k Ephrin-B phosphorylated at Tyr317 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr317 Ephrin-B. The immunolabeling of the Ephrin-B band is completely eliminated by treatment with lambda-Ptase.
Figure 1. Western blot of rat testes lysate showing specific immunolabeling of the ~46k Ephrin-B phosphorylated at Tyr331 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr331 Ephrin-B. The immunolabeling of the Ephrin-B band is completely eliminated by treatment with lambda-Ptase.
Human kidney; WB Suggested Anti-EFNB1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human kidney; EFNB1 antibody - middle region (ARP46450_P050) in Human kidney cells using Western Blot
Western blot analysis of Ephrin-B1 antibody (AP20226PU-N) in extracts from COS7 cells.
Rcombinant human EFNB1, 28-237aa, His-tagged
Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody AP55798PU-N (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody AP55798PU-N.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of Ephrin B1 (pY317) expression in HepG2 (A); NIH3T3 (B); mouse heart (C); rat skeletal muscle (D) whole cell lysates.
Western blot analysis of Ephrin B1/2 expression in HeLa (A); mouse hippocampus (B) whole cell lysates.
Western blot analysis of EFNB1/2 expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
WB Suggested Anti-EFNB1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human kidney
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA325424 | Ephrin-B1 antibody |
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| Rabbit Polyclonal EFNB1/2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP06387PU-N | Ephrin-B1 (+Ephrin-B2) antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP20226PU-N | Ephrin-B1 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP55798PU-N | Ephrin-B1/B2/B3 pTyr324 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
| AP55798PU-S | Ephrin-B1/B2/B3 pTyr324 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301886 | Ephrin-B1 |
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| Recombinant protein of human ephrin-B1 (EFNB1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP721089 | Ephrin-B1 |
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| Purified recombinant protein of Human ephrin-B1 (EFNB1) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401409 | EFNB1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205205L2V | Lenti ORF particles, Efnb1 (GFP-tagged) - Mouse ephrin B1 (Efnb1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205205L1V | Lenti ORF particles, Efnb1 (Myc-DDK-tagged) - Mouse ephrin B1 (Efnb1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||