EPSTI1 antibody
Principal name
EPSTI1 antibody
Alternative names for EPSTI1 antibody
Epithelial-stromal interaction protein 1
Ncbi ID
9606, NP_001002264, NP_001002264.1, NM_033255, NM_001002264, NP_150280, NP_083771.1, NM_178825, NM_001044257
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of EPSTI1 antibody
- Kit Component:
- - KN305307G1, Epsti1 gRNA vector 1 in pCas-Guide vector
- KN305307G2, Epsti1 gRNA vector 2 in pCas-Guide vector
- KN305307D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Nielsen,H.L., (2002) Genomics 79 (5), 703-710
Immunoperoxidase of monoclonal antibody to EPSTI1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Western Blot analysis of EPSTI1 expression in transfected 293T cell line by EPSTI1 monoclonal antibody (M01), clone 2A8.Lane 1: EPSTI1 transfected lysate(36.793 KDa).Lane 2: Non-transfected lysate.
EPSTI1 monoclonal antibody (M01), clone 2A8 Western Blot analysis of EPSTI1 expression in A-431( Cat # L015V1 ).
Detection limit for recombinant GST tagged EPSTI1 is approximately 0.03ng/ml as a capture antibody.
Human MCF-7; WB Suggested Anti-EPSTI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; EPSTI1 antibody - N-terminal region (ARP48427_P050) in Human MCF-7 cells using Western Blot
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
EPSTI1 monoclonal antibody, clone 2A8 Western Blot analysis of EPSTI1 expression in A-431.
Detection limit for recombinant GST tagged EPSTI1 is approximately 0.03 ng/ml as a capture antibody.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human placenta tissue, Primary antibody: (EPSTI1 Antibody) at dilution 1/1900, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (EPSTI1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (EPSTI1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
WB Suggested Anti-EPSTI1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysate
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305699 | EPSTI1 (transcript variant 1) |
||||
| Recombinant protein of human epithelial stromal interaction 1 (breast) (EPSTI1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP317229 | EPSTI1 (transcript variant 2) |
||||
| Recombinant protein of human epithelial stromal interaction 1 (breast) (EPSTI1), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
