ERCC4 antibody
Principal name
ERCC4 antibody
Alternative names for ERCC4 antibody
ERCC-4, ERCC11, XPF, DNA repair endonuclease XPF, DNA excision repair protein ERCC-4, DNA repair protein complementing XP-F cells, Xeroderma pigmentosum group F-complementing protein
Ncbi ID
NP_005227.1, 9606, NP_005227, AAI60102.1, NM_005236, BC026792, NM_015769, NP_056584.2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ERCC4 antibody
Xeroderma Pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer, and it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, is a 115 kDa protein that associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC-1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5’ incisions during DNA repair. The ERCC1-XPF endonuclease preferentially cleaves one strand of DNA between duplex and singlestranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.
General readings
1. Hodskinson MR et al. Mouse SLX4 is a tumor suppressor that stimulates the activity of the nuclease XPF-ERCC1 in DNA crosslink repair. Mol Cell 54:472-84 (2014). WB, IP ; Mouse .
Western blot using anibody cat.no. AP06807PU-N at 1:500 dilution: Lane1:HEK293T whole cell lysate, Lane2:sp2/0 whole cell lysate, Lane3:PC12 whole cell lysate
Formalin-fixed, paraffin embedded human tonsil stained with ERCC4 monoclonal antibody, clone 219 ( Cat # MAB3381 ).
Western blot analysis of whole cell lysate from LS174T with ERCC4 monoclonal antibody, clone 219 ( Cat # MAB3381 ).
Human PANC1; WB Suggested Anti-ERCC4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysate. ERCC4 is supported by BioGPS gene expression data to be expressed in PANC1.; ERCC4 antibody - middle region (ARP58272_P050) in Human PANC1 cells using Western Blot
Human Uterus (formalin-fixed, paraffin-embedded) stained with ERCC4 antibody AM20500PU-N at 10 µg/ml after heat-induced antigen retrieval.
Western blot analysis of ERCC4 expression in HeLa (A); KNRK (B); MCF7 (C) whole cell lysates.
Anti-ERCC4 mouse monoclonal antibody (TA503063) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4(RC223300).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4.
HEK293T cells transfected with either RC223300 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC4 antibody(TA503063), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-ERCC4 antibody(TA503063),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4.
HEK293T cells transfected with either RC223300 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC4 antibody(TA503163), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503261)
Anti-ERCC4 mouse monoclonal antibody (TA503261) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4(RC223300).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC4 monoclonal antibody.
HEK293T cells transfected with either RC223300 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC4 antibody(TA503261), and then analyzed by flow cytometry.
Anti-ERCC4 mouse monoclonal antibody (TA503262) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4(RC223300).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4.
HEK293T cells transfected with either RC223300 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC4 antibody(TA503262), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503294)
Anti-ERCC4 mouse monoclonal antibody (TA503294) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4(RC223300).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-ERCC4 monoclonal antibody at 1:200 dilution.
HEK293T cells transfected with either RC223300 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC4 antibody(TA503294), and then analyzed by flow cytometry.
Anti-ERCC4 mouse monoclonal antibody (TA503314) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4(RC223300).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC4 monoclonal antibody.
HEK293T cells transfected with either RC223300 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC4 antibody(TA503314), and then analyzed by flow cytometry.
Anti-ERCC4 mouse monoclonal antibody (TA503324) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4(RC223300).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC4 monoclonal antibody.
HEK293T cells transfected with either RC223300 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC4 antibody(TA503324), and then analyzed by flow cytometry.
Anti-ERCC4 mouse monoclonal antibody (TA503341) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4(RC223300).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4.
HEK293T cells transfected with either RC223300 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC4 antibody(TA503341), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded human uterus using anti-XPF clone UMAB20 mouse monoclonal antibody (UM500020) 1:50 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with TEE pH9.0 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear staining is seen in the epithiel cells of the uterine gland
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-XPF mouse monoclonal antibody (clone UMAB20). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-XPF (UMAB20) very specifically recognizes XPF antigen on OriGene protein microarray chip.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-XPF mouse monoclonal antibody (clone UMAB21). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-XPF (UMAB21) very specifically recognizes XPF antigen on OriGene protein microarray chip.
Immunohistochemical staining of paraffin-embedded human breast cancer using anti-XPF clone UMAB20 mouse monoclonal antibody (UM500020) 1:50 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with TEE pH9.0 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear staining is seen in the tumor cells.
Immunohistochemical staining of paraffin-embedded human lung cancer using anti-XPF clone UMAB20 mouse monoclonal antibody (UM500022) 1:50 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with TEE pH9.0 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear staining is seen in the tumor cells.
Immunohistochemical staining of paraffin-embedded human ovarian cancer using anti-XPF clone UMAB20 mouse monoclonal antibody (UM500022) 1:50 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with TEE pH9.0 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear staining is seen in the tumor cells.
Immunohistochemical staining of paraffin-embedded human pancreas using anti-XPF clone UMAB20 mouse monoclonal antibody (UM500022) 1:50 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with TEE pH9.0 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear staining is seen in the pancreatic cells.
Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-XPF clone UMAB20 mouse monoclonal antibody (UM500022) 1:50 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with TEE pH9.0 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear staining is seen in the tumor cells.
Immunohistochemical staining of paraffin-embedded human thyroid using anti-XPF clone UMAB20 mouse monoclonal antibody (UM500022) 1:50 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with TEE pH9.0 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear staining is seen in the thyroid cells.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-XPF mouse monoclonal antibody (clone UMAB22). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-XPF (UMAB22) very specifically recognizes XPF antigen on OriGene protein microarray chip.
Western blot analysis of extracts from 293 cells, using XPF antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-ERCC4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: PANC1 cell lysateERCC4 is supported by BioGPS gene expression data to be expressed in PANC1
Western blot analysis on 293 cell lysate using XPF Antibody
- Quick-Filters:
- Highly Validated (11)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM500020 | ERCC4 antibody |
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| XPF mouse monoclonal antibody,clone UMAB20 | |||||||
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Mouse | IgG2a | UMAB20 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Highly validated antibody!
|
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| UM500021 | ERCC4 antibody |
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| XPF mouse monoclonal antibody,clone UMAB21 | |||||||
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Mouse | IgG1 | UMAB21 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
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| UM500022 | ERCC4 antibody |
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| XPF mouse monoclonal antibody,clone UMAB22 | |||||||
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Mouse | IgG2b | UMAB22 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| UM570020 | ERCC4 antibody |
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| XPF mouse monoclonal antibody,clone UMAB20 | |||||||
|
Mouse | IgG2a | UMAB20 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, P, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
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| UM570021 | ERCC4 antibody |
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| XPF mouse monoclonal antibody,clone UMAB21 | |||||||
|
Mouse | IgG1 | UMAB21 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, P, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
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| UM570022 | ERCC4 antibody |
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| XPF mouse monoclonal antibody,clone UMAB22 | |||||||
|
Mouse | IgG2b | UMAB22 | Purified | Hu | F, ICC/IF, P, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| TA503063 | ERCC4 antibody |
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| ERCC4 mouse monoclonal antibody, clone OTI2D4 (formerly 2D4) | |||||||
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Mouse | IgG2b | OTI2D4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA503261 | ERCC4 antibody |
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| ERCC4 mouse monoclonal antibody, clone OTI4E11 (formerly 4E11) | |||||||
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Mouse | IgG2a | OTI4E11 | Purified | Hu, Ms | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +16 additional images |
Highly validated antibody!
|
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| TA503294 | ERCC4 antibody |
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| ERCC4 mouse monoclonal antibody, clone OTI6E6 (formerly 6E6) | |||||||
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Mouse | IgG2b | OTI6E6 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA503314 | ERCC4 antibody |
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| ERCC4 mouse monoclonal antibody, clone OTI2A1 (formerly 2A1) | |||||||
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Mouse | IgG2a | OTI2A1 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA503324 | ERCC4 antibody |
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| ERCC4 mouse monoclonal antibody, clone OTI3H10 (formerly 3H10) | |||||||
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Mouse | IgG1 | OTI3H10 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA312204 | ERCC4 antibody |
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| Rabbit polyclonal anti-XPF antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA347507 | ERCC4 antibody |
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| Rabbit Polyclonal Anti-XPF Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA503163 | ERCC4 antibody |
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| ERCC4 mouse monoclonal antibody, clone OTI3H7 (formerly 3H7) | |||||||
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Mouse | IgG1 | OTI3H7 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA503262 | ERCC4 antibody |
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| ERCC4 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2) | |||||||
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Mouse | IgG1 | OTI1B2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA503341 | ERCC4 antibody |
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| ERCC4 mouse monoclonal antibody, clone OTI2C2 (formerly 2C2) | |||||||
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Mouse | IgG2b | OTI2C2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AM20500PU-N | ERCC4 antibody |
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Mouse | IgG2 | Purified | Hu | P, WB |
50 µl /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP06807PU-N | ERCC4 antibody |
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Rabbit | Aff - Purified | Hu, Ms | E, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP323300 | ERCC4 |
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| Recombinant protein of human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401605 | ERCC4 overexpression lysate |
|
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0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |