ERCC8 antibody
Principal name
ERCC8 antibody
Alternative names for ERCC8 antibody
CKN1, CSA, ERCC-8, DNA excision repair protein ERCC-8, Cockayne syndrome WD repeat protein CSA
Ncbi ID
NP_000073.1, NP_000073, 9606, NM_000082, AAH09793.1, NM_001007234, NP_001007235, NP_082318.2, NM_028042
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ERCC8 antibody
- Kit Component:
- - KN305329G1, Ercc8 gRNA vector 1 in pCas-Guide vector
- KN305329G2, Ercc8 gRNA vector 2 in pCas-Guide vector
- KN305329D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Guillem, V.M., et al. Am. J. Hematol. 85(7):482-486(2010)
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press.
Kamenisch, Y., et al. J. Exp. Med. 207(2):379-390(2010)
Laugel, V., et al. Hum. Mutat. 31(2):113-126(2010).
Human Liver; ERCC8 antibody - C-terminal region (ARP31542_P050) in Human Liver cells using Immunohistochemistry
Human Muscle; HumanMuscle; ERCC8 antibody - C-terminal region (ARP31542_P050) in Human Muscle cells using Immunohistochemistry
Hum. Fetal Heart; Host:Rabbit. Target Name:ERCC8. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; ERCC8 antibody - C-terminal region (ARP31542_P050) in Hum. Fetal Heart cells using Western Blot
Hum. Fetal Brain; Host:Rabbit. Target Name:ERCC8. Sample Tissue:Human Fetal Brain. Antibody Dilution: 1.0ug/ml.; ERCC8 antibody - C-terminal region (ARP31542_P050) in Hum. Fetal Brain cells using Western Blot
Hum. Adult Placenta; Host:Rabbit. Target Name:ERCC8. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; ERCC8 antibody - C-terminal region (ARP31542_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Lung; Host:Rabbit. Target Name:ERCC8. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; ERCC8 antibody - C-terminal region (ARP31542_P050) in Hum. Fetal Lung cells using Western Blot
Human Jurkat; WB Suggested Anti-ERCC8 Antibody Titration: 0.1-5.0ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; ERCC8 antibody - C-terminal region (ARP31542_P050) in Human Jurkat cells using Western Blot
Human HepG2; Host:Rabbit. Target Name:ERCC8. Sample Tissue:Human HepG2. Antibody Dilution: 1.0ug/ml. ERCC8 is supported by BioGPS gene expression data to be expressed in HepG2.; ERCC8 antibody - C-terminal region (ARP31542_P050) in Human HepG2 cells using Western Blot
Human MCF7; Host: Rabbit. Target Name: ERCC8. Sample Tissue: MCF7 Whole Cell lysates. Antibody Dilution: 1.0ug/ml.; ERCC8 antibody - C-terminal region (ARP31542_P050) in Human MCF7 cells using Western Blot
Human Muscle; WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human muscle; ERCC8 antibody - N-terminal region (ARP32149_P050) in Human Muscle cells using Western Blot
Western Blot analysis of ERCC8 expression in transfected 293T cell line (H00001161-T02) by ERCC8 MaxPab polyclonal antibody.Lane 1: ERCC8 transfected lysate(44.10 KDa).Lane 2: Non-transfected lysate.
Human Placenta; WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Placenta; ERCC8 antibody - N-terminal region (ARP57955_P050) in Human Placenta cells using Western Blot
Human HepG2; WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. ERCC8 is supported by BioGPS gene expression data to be expressed in HepG2.; ERCC8 antibody - middle region (ARP58570_P050) in Human HepG2 cells using Western Blot
Immunoprecipitation of ERCC8 transfected lysate using anti-ERCC8 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ERCC8 MaxPab mouse polyclonal antibody (B02) (H00001161-B02).
Human HeLa; WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Hela cell lysate; ERCC8 antibody - middle region (ARP57956_P050) in Human HeLa cells using Western Blot
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of paraformaldehyde-fixed A431, merged with DNA probe, using ERCC8 (CSA) antibody at 1:200 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC8 (CSA) antibody at 1:200 dilution.
Western blot: Sample (30 µg of whole cell lysate). A:NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.
Western blot: Sample (30 µg of whole cell lysate). A:293T. B:A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500
Human Jurkat; WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; ERCC8 antibody - middle region (ARP58571_P050) in Human Jurkat cells using Western Blot
Formalin fixed and paraffin embedded human lung carcinoma reacted with ERCC8 Antibody Cat.-No AP51450PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of ERCC8 Antibody Cat.-No AP51450PU-N in mouse spleen tissue lysates (35ug/lane). This demonstrates the ERCC8 antibody detected the ERCC8 protein (arrow).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ERCC8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808524)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERCC8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808524)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERCC8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808524)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808524)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-ERCC8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808524)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC8 (RC203124, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC8.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC8 (RC203124, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC8.(1:2000)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808577)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERCC8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808577)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC8 (RC203124, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC8.(1:500)
Sample(30 ug of whole cell lysate). A:293T. B:A431. 12% SDS PAGE. TA308479 diluted at 1:500
ERCC8 antibody [N2C2], Internal detects ERCC8 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC8(CSA)(TA308479) antibody at 1:200 dilution.
ERCC8 antibody detects ERCC8 protein by Western blot analysis. A. 30 ug C8D30 whole cell lysate/extract. 10 % SDS-PAGE. ERCC8 antibody (TA308521) dilution: 1:1000
ERCC8 antibody detects ERCC8 protein by Western blot analysis. A. 30 ug Rat2 whole cell lysate/extract. 10 % SDS-PAGE. ERCC8 antibody (TA308521) dilution: 1:1000
Sample (30ug whole cell lysate). A:MOLT4 . 10% SDS PAGE. TA308521 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ERCC8(TA308521) antibody at 1:200 dilution.
WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human muscle
WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysateERCC8 is supported by BioGPS gene expression data to be expressed in HepG2
WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Hela cell lysate
WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308521 | ERCC8 antibody |
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| Rabbit Polyclonal antibody to CSA (excision repair cross-complementing rodent repair deficiency, complementation group 8) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA808524 | ERCC8 antibody |
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| ERCC8 mouse monoclonal antibody, clone OTI2G1 (formerly 2G1) | |||||||
 |
Mouse | IgG2a | OTI2G1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| AP23086PU-N | ERCC8 (106-300) antibody |
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 |
Rabbit | Aff - Purified | Bov, Hu, Ms, Rt | ICC/IF, P, WB |
50 µl /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA308479 | ERCC8 antibody |
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| Rabbit polyclonal antibody to CSA (excision repair cross-complementing rodent repair deficiency, complementation group 8) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA808562 | ERCC8 antibody |
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| ERCC8 mouse monoclonal antibody, clone OTI9B11 (formerly 9B11) | |||||||
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Mouse | IgG2b | OTI9B11 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808577 | ERCC8 antibody |
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| ERCC8 mouse monoclonal antibody, clone OTI1G2 (formerly 1G2) | |||||||
 |
Mouse | IgG1 | OTI1G2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP51450PU-N | ERCC8 (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203124 | ERCC8 - human gene knockout kit via CRISPR |
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 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203124BN | ERCC8 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203124LP | ERCC8 - human gene knockout kit via CRISPR |
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|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203124RB | ERCC8 - human gene knockout kit via CRISPR |
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|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760049 | ERCC8 (full length, N-term HIS tag, transcript variant 3) |
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| Recombinant protein of human excision repair cross-complementing rodent repair deficiency, complementation group 8 (ERCC8), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY423459 | ERCC8 overexpression lysate |
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY424939 | ERCC8 overexpression lysate |
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|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY424984 | ERCC8 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206229L2V | Lenti ORF particles, Ercc8 (GFP-tagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8), 200 uL, >10^7 TU/mL |
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|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206229L1V | Lenti ORF particles, Ercc8 (Myc-DDK-tagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
