ESM1 antibody
Principal name
ESM1 antibody
Alternative names for ESM1 antibody
Endothelial cell-specific molecule 1, Endocan
Ncbi ID
9606, NP_076101, NP_008967, NP_008967.1, NP_076101.1, NM_007036, NM_001135604, NM_023612, BC020038, NM_022604
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of ESM1 antibody
Endocan, also known as endothelial cell-specific molecule1 (ESM1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung. Endocan expression has also been detected in different epithelia and in adipocytes. The expression of endocan is up-regulated by TNFα, IL1β or lipopolysaccharide and down-regulated by IFNγ. The human Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues. The DS chain is covalently attached to serine 137. Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen1, LFA1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM1 and reducing LFA1mediated leukocyte activation. Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity. Genetically engineered cells overexpressing Endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo. Circulating Endocan can be detected in the serum from healthy subjects. In patients with lung cancer or acute and severe sepsis, elevated Endocan concentrations have been reported.
General readings
Scherpereel A., et al. (2003) Cancer Res. 63:6084.Bechard D., et al. (2000) J Vasc Res. 37:417.
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Medicine Göttingen, Germany.
Lane 1: MWM (kDa),
Lane 2: rh ESM1,
Lane 3: rm ESM1.
Lane 1: MWM (kDa),
Lane 2: rm ESM1,
Lane 3: rh ESM1.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP60002PU-L | ESM1 antibody |
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 |
Rabbit | IgG | Purified | Hu | C, ICC/IF, WB |
0.2 mg /
€370.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AP60002PU-N | ESM1 antibody |
||||||
|
Rabbit | IgG | Purified | Hu | C, ICC/IF, WB |
0.1 mg /
€270.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP60003PU-L | ESM1 antibody |
||||||
 |
Rabbit | IgG | Purified | Ms | WB |
0.2 mg /
€370.00
|
|
| OriGene Technologies GmbH | |||||||
| AP60003PU-N | ESM1 antibody |
||||||
|
Rabbit | IgG | Purified | Ms | WB |
0.1 mg /
€270.00
|
|
| OriGene Technologies GmbH | |||||||
| DM3543P | ESM1 antibody |
||||||
 |
Rat | IgG2 | 5F44 | Purified | Ms | WB |
0.1 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP720639 | ESM1 (transcript variant 1) |
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| Purified recombinant protein of Human endothelial cell-specific molecule 1 (ESM1), transcript variant 1 | |||||
|
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
| AR60002PU-N | ESM1 (His-tag) |
||||
 |
Human | Purified | > 95 % by SDS-PAGE and Silver staining | Insect cells |
50 µg /
€350.00
|
| OriGene Technologies GmbH | |||||
| AR60003PU-N | ESM1 (His-tag) |
||||
 |
Mouse | Purified | > 95 % by SDS-PAGE and Silver staining | Insect cells |
50 µg /
€350.00
|
| OriGene Technologies GmbH | |||||
ELISA Kits
| Catalog No. | ||
|---|---|---|
| EA100477 | Human ESM1/Endocan ELISA Kit |
|
|
1 kit /
€455.00
|
|
| OriGene Technologies, Inc. | ||
| EA100478 | Mouse ESM1/Endocan ELISA Kit |
|
|
1 kit /
€455.00
|
|
| OriGene Technologies, Inc. |
Lysates
| Catalog No. | ||
|---|---|---|
| LY402078 | ESM1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY427633 | ESM1 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201703L2V | Lenti ORF particles, Esm1 (GFP-tagged) - Mouse endothelial cell-specific molecule 1 (cDNA clone MGC:28355 IMAGE:4018658), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€840.00
|
|
| OriGene Technologies, Inc. | ||
| MR201704L2V | Lenti ORF particles, Esm1 (GFP-tagged) - Mouse endothelial cell-specific molecule 1 (Esm1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201703L1V | Lenti ORF particles, Esm1 (Myc-DDK-tagged) - Mouse endothelial cell-specific molecule 1 (cDNA clone MGC:28355 IMAGE:4018658), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€740.00
|
|
| OriGene Technologies, Inc. | ||
| MR201704L1V | Lenti ORF particles, Esm1 (Myc-DDK-tagged) - Mouse endothelial cell-specific molecule 1 (Esm1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201802L2V | Lenti ORF particles, Esm1 (GFP-tagged ORF) - Rat endothelial cell-specific molecule 1 (Esm1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR201802L1V | Lenti ORF particles, Esm1 (Myc-DDK-tagged ORF) - Rat endothelial cell-specific molecule 1 (Esm1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||