Estrogen receptor beta antibody
Principal name
Estrogen receptor beta antibody
Alternative names for Estrogen receptor beta antibody
ER-beta,ESR2, ESTRB, NR3A2, Nuclear receptor subfamily 3 group A member 2
SwissProt ID
O08537 (Mouse), Q62986 (Rat), Q92731 (Human), Q9PTU5 (Chick), Q9TU15 (Sheep), Q9XSB5 (Bovin), Q9XSW2 (Pig)
Ncbi ID
NP_001428, NP_001035365, 9606, NP_001035365.1, NP_997590, AAH24181, 10090, NM_010157, NM_001040275, NM_001040276, NM_001437
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)
Background of Estrogen receptor beta antibody
Estrogen, a steroid hormone, is a key regulator of growth and differentiation in a broad range of target tissues and, it is also implicated in breast and endometrial cancer and osteoporosis (1). Like all steroid hormones, estrogen readily diffuses across cell membrane and binds to and activates estrogen receptors (α and β) which then up-regulate the expression of many genes. These receptors belong to the superfamily of nuclear receptors, more specifically to the family of steroid receptors that act as ligand-regulated transcription factors (2). Estrogen binding receptor protein was first discovered in the early sixties, followed by cloning of this receptor in the mid-eighties. In 996, this receptor was renamed estrogen receptor alpha (ER-alpha) due to cloning of an additional estrogen receptor from rat prostrate. This novel receptor was designated estrogen receptor beta (ER-beta) (3). ER-beta is located on mouse chromosome 12 D1-D3. Recent reports have shown that ER-beta is important for the prevention of age-related hearing loss, proper cAMP signaling pathway, polyovular follicles in mouse ovaries and also important in regulating tyrosine hydroxylase expression in the moue locus coeruleus (4).
Any disruption in estrogen receptor beta signaling has been shown to cause negative effects on ovulation and fertility (4). ER-beta deficiency in mice was found to be associated with increase in small intestine tumorigenesis. ER-beta deficiency also leads to increase epithelial proliferation, decreased apoptosis, and accumulation of incompletely differentiated cells in mouse ventral prostrates (5). In male mice, ER-beta deletion affected the structure of their arteries. ER-beta is expressed in kidney, spleen, testis and skeletal muscles. It is approximately a 72kDa protein (567 amino acids).
General readings
Brandenberger,A.W., et al., (1997) J. Clin. Endocrinol. Metab. 82 (10), 3509-3512
Human HepG2; WB Suggested Anti-ESR2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that ESR2 is expressed in HepG2.; ESR2 antibody - N-terminal region (ARP31652_P050) in Human HepG2 cells using Western Blot
Photomicrograph of human ovary immunostained for estrogen receptor beta using monoclonal antibody Cat.-No SM1682 (Clone PPG5/10).
Staining of formalin fixed, paraffin embedded Human Placenta with Mouseanti Human estrogen Receptor (Cat.-No SM1682 Clone PPG5/10).
Staining of formalin fixed, paraffin embedded Human Placenta with Mouse anti Human estrogen Receptor (Cat.-No SM1682 Clone PPG5/10).
Staining of paraffin embedded Human Breast cancer tissue with Mouse anti Human Estrogen Receptor (Cat.-No SM1682 Clone PPG5/10) following antigen retrieval with EDTA. Detection was performed with Histar detection kit.
Staining of formalin fixed, paraffin embedded Human Placenta with Mouse anti Human estrogen Receptor (Cat.-No SM1682 Clone PPG5/10).
Staining of formalin fixed, paraffin embedded Human Placenta with Mouse anti Human estrogen Receptor (Cat.-No SM1682 Clone PPG5/10).
Staining of paraffin embedded Human Breast cancer tissue with Mouse anti Human Estrogen Receptor (Cat.-No SM1682 Clone PPG5/10) following antigen retrieval with EDTA. Detection was performed with Histar detection kit.
Photomicrograph of human ovary immunostained for estrogen receptor beta using monoclonal antibody SM1682X.
Detection limit for recombinant GST tagged ESR2 is approximately 0.3ng/ml as a capture antibody.
ESR2 polyclonal antibody (A01), Lot # ABNOVA060605QCS1 Western Blot analysis of ESR2 expression in MES-SA/Dx5 ( Cat # L021V1 ).
Human HepG2; WB Suggested Anti-ESR2 Antibody. . Titration: 1.25 ug/ml. . Positive Control: HepG2 Whole Cell. There is BioGPS gene expression data showing that ESR2 is expressed in HepG2.; ESR2 antibody - N-terminal region (ARP31652_T100) in Human HepG2 cells using Western Blot
Western blot (WB) analysis of ER-beta antibody (Cat.-No.: AP06806PU-N) in extracts from HeLa cells.
Western Blot analysis of ESR2 expression in transfected 293T cell line (H00002100-T03) by ESR2 MaxPab polyclonal antibody.Lane 1: ESR2 transfected lysate(35.90 KDa).Lane 2: Non-transfected lysate.
IHC : Human breast cancer tissue stained with ESR2 polyclonal antibody ( Cat # PAB12682 ) at 1 : 200 for 10 min at RT. Staining of formalin-fixedtissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
The whole cell lysates derived from MCF-7 were immunoblotted by ESR2 polyclonal antibody ( Cat # PAB12682 ) at 1 : 500 ( Lane 1 ). The immunoreactive band is blocked by pre-incubation of immunizing peptide ( Lane 2 ).
Western Blot of Estrb-1. Estrb-1 antibody (Cat#AP09861PU-N) at 1/500 dilution.
Hum. Adult Placenta; Host: Rabbit. Target Name: CHAD . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; ESR2 antibody - middle region (ARP32258_P050) in Hum. Adult Placenta cells using Western Blot
Human 293T; Host:Rabbit. Target Name:ESR2. Sample Tissue:293T. Antibody Dilution: 1.0ug/ml.; ESR2 antibody - middle region (ARP32258_P050) in Human 293T cells using Western Blot
Hum. Fetal Muscle; Host:Rabbit. Target Name:ESR2. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; ESR2 antibody - middle region (ARP32258_P050) in Hum. Fetal Muscle cells using Western Blot
Human HeLa; WB Suggested Anti-ESR2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; ESR2 antibody - middle region (ARP32258_P050) in Human HeLa cells using Western Blot
Rat Brain; ESR2 antibody - N-terminal region (ARP35863_P050) in Rat Brain cells using Immunohistochemistry
Hum. Adult Placenta; Host:Rabbit. Target Name:ESR2. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; ESR2 antibody - N-terminal region (ARP35863_P050) in Hum. Adult Placenta cells using Western Blot
Human ACHN; WB Suggested Anti-ESR2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: ACHN cell lysate; ESR2 antibody - N-terminal region (ARP35863_P050) in Human ACHN cells using Western Blot
Hum. Fetal Muscle; Host:Rabbit. Target Name:ESR2. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; ESR2 antibody - N-terminal region (ARP35863_P050) in Hum. Fetal Muscle cells using Western Blot
Prostate, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Cat.-No. AM21050PU-N (Clone 4ERbeta-2D7) ESTRB antibody staining of Human Hypothalamus Paraffin Section.
Formalin-Fixed, Paraffin-Embedded ovarian cancer stained with Estrogen receptor beta Antibody Cat.-No AM32846PU (Clone ERb455).
Formalin-Fixed, Paraffin-Embedded breast cancer stained with Estrogen receptor beta Antibody Cat.-No AM32846PU (Clone ERb455).
Western blot analysis of extracts from HepG2 tissue using Estrogen Receptor- beta (Phospho-Ser105) antibody AP55945PU-N.The lane on the right is treated with the antigen-specific peptide.
ELISA: Estrogen Receptor beta Antibody
Western blot analysis of Estrogen Receptor beta (pS105) expression in MCF7 (A); HeLa (B) whole cell lysates.
Immunofluorescent analysis of Estrogen Receptor beta (pS105) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
(1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ESR2 Antibody (Center) (Cat. #TA324615) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ESR2 antibody detected the ESR2 protein (arrow).
Flow cytometric analysis of HepG2 cells using ESR2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ESR2 Antibody (C-term) (Cat. #TA324671) western blot analysis in MCF-7 cell line and human placenta tissue lysates (35ug/lane).This demonstrates the ESR2 antibody detected the ESR2 protein (arrow).
ESR2 Antibody (C-term) (Cat. #TA324672) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ESR2 antibody detected the ESR2 protein (arrow).
Western blot analysis of Estrogen Receptor- beta phosphorylation expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of Estrogen Receptor-beta expression in EGF treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using ESR2 antibody.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (ESR2 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (ESR2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using (ESR2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blot analysis of extracts from MCF7 cells using Estrogen Receptor-alpha (phospho-Ser167) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-alpha (phospho-Ser167) antibody.
Western blot analysis of extracts from MCF7 cells using Estrogen Receptor-alpha (phospho-Ser118) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-alpha (phospho-Ser118) antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309968 | Estrogen receptor beta antibody |
||||||
| Rabbit anti-ESR2 (Estrogen Receptor-beta, Phospho-Ser118) polyclonal antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Ms | P, WB |
0.1 mg /
€355.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA309969 | Estrogen receptor beta antibody |
||||||
| Rabbit anti-ESR2 (Estrogen Receptor-beta, Phospho-Ser167) polyclonal antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu | P, WB |
0.1 mg /
€355.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA311208 | Estrogen receptor beta (417-428) antibody |
||||||
| Goat Anti-Estrogen receptor beta (aa417-428) Antibody | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA324615 | Estrogen receptor beta antibody |
||||||
| Rabbit polyclonal ESR2 Antibody (Center) | |||||||
 |
Rabbit | IgG | Purified | Bov, Chk, Hu, Por, Rt | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA324671 | Estrogen receptor beta antibody |
||||||
| Rabbit polyclonal ESR2 Antibody(C-term) | |||||||
 |
Rabbit | IgG | Purified | Bov, Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324672 | Estrogen receptor beta antibody |
||||||
| Rabbit polyclonal ESR2 Antibody(C-term) | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327832 | Estrogen receptor beta antibody |
||||||
| Rabbit Polyclonal Phospho-Estrogen Receptor- beta (Ser105) Antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327896 | Estrogen receptor beta antibody |
||||||
| Rabbit Polyclonal Estrogen Receptor-beta Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA332550 | Estrogen receptor beta antibody |
||||||
| Rabbit anti-ESR2 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA349939 | Estrogen receptor beta antibody |
||||||
| Rabbit Polyclonal Anti-ESR2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AM21050PU-N | Estrogen receptor beta (F region) antibody |
||||||
 |
Mouse | IgG1 | ERbeta-2D7 | Aff - Purified | Hu, Ms | C, P |
0.25 mg /
€440.00
|
| OriGene Technologies GmbH | |||||||
| AP06806PU-N | Estrogen receptor beta antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP23349PU-N | Estrogen receptor beta (N-term) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€390.00
|
|
| OriGene Technologies GmbH | |||||||
| AP55945PU-N | Estrogen receptor beta pSer105 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| AP55945PU-S | Estrogen receptor beta pSer105 antibody |
||||||
|
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN212665 | ESR2 - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN212665BN | ESR2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN212665LP | ESR2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN212665RB | ESR2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP710031 | Estrogen receptor beta (full length, C-term DDK tag) |
||||
| Recombinant protein of human estrogen receptor 2 (ER beta) (ESR2), full length, with C-terminal DDK tag, expressed in sf9 cells. | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
