ETFB antibody
Principal name
ETFB antibody
Alternative names for ETFB antibody
Beta-ETF, Electron transfer flavoprotein subunit beta
Ncbi ID
9606, NP_001976, NP_001014763, NP_001014763.1, NM_001014763, NP_080971.2, NM_026695, NM_001004220, NM_001985
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ETFB antibody
- Kit Component:
- - KN305394G1, Etfb gRNA vector 1 in pCas-Guide vector
- KN305394G2, Etfb gRNA vector 2 in pCas-Guide vector
- KN305394D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Ohkuma A, Noguchi S, Sugie H, Malicdan MC, Fukuda T, Shimazu K, López LC, Hirano M, Hayashi YK, Nonaka I, Nishino I, Clinical and genetic analysis of lipid storage myopathies. Muscle & nerve 2009 Mar 39 (3): 333-42.
PMID: 19208393
ETFB MaxPab polyclonal antibody. Western Blot analysis of ETFB expression in human liver.
Western Blot analysis of ETFB expression in transfected 293T cell line (H00002109-T01) by ETFB MaxPab polyclonal antibody.Lane 1: ETFB transfected lysate(28.05 KDa).Lane 2: Non-transfected lysate.
ETFB MaxPab polyclonal antibody. Western Blot analysis of ETFB expression in human liver.
Western Blot analysis of ETFB expression in transfected 293T cell line (H00002109-T01) by ETFB MaxPab polyclonal antibody.Lane 1: ETFB transfected lysate(28.05 KDa).Lane 2: Non-transfected lysate.
Human MCF7; Host:Rabbit. Target Name:ETFB. Sample Tissue:Human MCF7. Antibody Dilution: 1.0ug/ml. ETFB is supported by BioGPS gene expression data to be expressed in MCF7.; ETFB antibody - C-terminal region (ARP54442_P050) in Human MCF7 cells using Western Blot
Human Heart; WB Suggested Anti-ETFB Antibody Titration: 0.2-1 ug/ml. Positive Control: Human heart; ETFB antibody - C-terminal region (ARP54442_P050) in Human Heart cells using Western Blot
AP21283PU-N ETFB antibody staining of Human Liver lysate at 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AP21283PU-N ETFB antibody staining paraffin embedded Human Liver at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Recombinant human ETFB, 1-255aa, His-tagged
Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308553 diluted at 1:10000.
Sample (30 ug of whole cell lysate). A: MOLT4 . 12% SDS PAGE. TA308553 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-ETF(TA308553) antibody at 1:200 dilution.
(0.01ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA311218 (3.8µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Host: Rabbit; Target Name: ETFB; Sample Tissue: COLO205 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-ETFB Antibody Titration: 0.2-1 ug/ml; Positive Control: Human heart
Host: Rabbit; Target Name: ETFB; Sample Tissue: Human MCF7; Antibody Dilution: 1.0 ug/mlETFB is supported by BioGPS gene expression data to be expressed in MCF7
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: LoVo cells, Hela cells, Primary antibody: (ETFB Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (ETFB Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (ETFB Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP21283PU-N | ETFB (152-165) antibody |
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, P, WB |
0.1 mg /
€380.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
OriGene recommends this product for Western blotting. |
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| TA308553 | ETFB antibody |
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| Rabbit Polyclonal antibody to Beta ETF (electron-transfer-flavoprotein, beta polypeptide) | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA311218 | ETFB (231-243) antibody |
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| Goat Anti-ETFB (aa 231-243) Antibody | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, P, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA351170 | ETFB antibody |
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| Rabbit Polyclonal Anti-ETFB Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AP54911SU-N | ETFB antibody |
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Rabbit | Serum | Hu | C, E, WB |
0.2 ml /
€430.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310894 | ETFB (transcript variant 1) |
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| Recombinant protein of human electron-transfer-flavoprotein, beta polypeptide (ETFB), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY423075 | ETFB overexpression lysate |
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY425362 | ETFB overexpression lysate |
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220480L2V | Lenti ORF particles, Etfb (GFP-tagged) - Mouse electron transferring flavoprotein, beta polypeptide (Etfb), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR220480L1V | Lenti ORF particles, Etfb (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, beta polypeptide (Etfb), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209202L2V | Lenti ORF particles, Etfb (GFP-tagged ORF) - Rat electron-transfer-flavoprotein, beta polypeptide (Etfb), 200 uL, >10^7 TU/mL |
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|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR209202L1V | Lenti ORF particles, Etfb (Myc-DDK-tagged ORF) - Rat electron-transfer-flavoprotein, beta polypeptide (Etfb), 200 uL, >10^7 TU/mL |
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|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
