ETS1 antibody
Principal name
ETS1 antibody
Alternative names for ETS1 antibody
Protein C-ets-1, EWSR2, p54
Ncbi ID
NP_005229.1, NP_005229, 9606, NP_035938.2, NM_001143820, NM_005238, NM_001162422, NM_012555, NM_011808
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), ELISA (detection) (E(detection))
Background of ETS1 antibody
Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element. Evidence indicates that the DNA binding activity by Ets-1 is regulated at the level of phosphorylation.
General readings
Harris, T.A., et al. Arterioscler. Thromb. Vasc. Biol. 30(10):1990-1997(2010)
He, C.F., et al. Lupus 19(10):1181-1186(2010)
Russell, L., et al. Cytokine 51(3):217-226(2010)
Li, T., et al. Pharm Biol 48(2):161-165(2010)
Kunderfranco, P., et al. PLoS ONE 5 (5), E10547 (2010).
Immunohistochemistry (IHC) analysis of ETS1 antibody (Cat.-No.: AP06524PU-N) in paraffin-embedded human skin tissue.
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Confocal immunofluorescent analysis of ETS1 Antibody (N-term) Cat.-No AP51470PU-N with MCF-7 cell followed by Alexa Fluor®488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of ETS1 Antibody (N-term) Cat.-No AP51470PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the ETS1 antibody detected the ETS1 protein (arrow).
Flow cytometric analysis of Jurkat cells using ETS1 Antibody (N-term) Cat.-No AP51470PU-N (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Proximity Ligation Analysis (PLA) of protein-protein interactions between ETS1 and MAPK1 HeLa cells were stained with anti-ETS1 rabbit purified polyclonal 1:1200 and anti-MAPK1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
ETS1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ETS1 expression in mouse intestine.
ETS1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ETS1 expression in human spleen.
Western Blot analysis of ETS1 expression in transfected 293T cell line (H00002113-T02) by ETS1 MaxPab polyclonal antibody.Lane 1: ETS1 transfected lysate(50.40 KDa).Lane 2: Non-transfected lysate.
Human Lung; . Rabbit Anti-ETS1 Antibody. . Catalog Number:. P100604 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Human kidney; ETS1 antibody - N-terminal region (P100604_T100) in Human kidney cells using Immunohistochemistry
Human pancreas; Human Pancrease; ETS1 antibody - N-terminal region (P100604_T100) in Human pancreas cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-ETS1 Antibody Titration: 1.0ug/ml. Positive Control: HepG2 cell lysate; ETS1 antibody - N-terminal region (P100604_T100) in Human HepG2 cells using Western Blot
Immunohistochemical analysis of paraffin-embedded human Colon, Adipocytes tissues using anti-ETS1 antibody Cat.-No AM06415SU-N
Immunofluorescence analysis of NIH/3T3 cells using anti-ETS1 antibody Cat.-No AM06415SU-N (green). Blue: DRAQ5 fluorescent DNA dye.
Western blot analysis using ETS1 antibody Cat.-No AM06415SU-N against Jurkat (Lane 1), HepG2 (Lane 2) and ETS1-hIgGFc transfected HEK293 (Lane 3) cell lysate.
Flow Cytometric analysis of Jurkat cells using anti-ETS1 antibody Cat.-No AM06415SU-N (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and breast tumor (B) uing ETS1 antibody Cat.-No AM06416SU-N
Western blot analysis using ETS1 antibody Cat.-No AM06416SU-N against Jurkat (Lane 1), HepG2 (Lane 2) and ETS1-hIgGFc transfected HEK293 (Lane 3) cell lysate.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody AP55698PU-N (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody AP55698PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence staining of methanol-fixed HeLa cells using ETS1 (Phospho-Thr38) Antibody AP55698PU-N.
Immunohistochemical analysis of ETS1 (pT38) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ETS1 (pT38) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ETS1 (pT38) expression in LO2 (A); Jurkat (B); HepG2 (C); NIH3T3 (D); rat liver (E) whole cell lysates.
Western blot analysis of ETS1 expression in HeLa (A); HEK293T (B) whole cell lysates.
Immunohistochemical analysis of ETS1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ETS1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of A549 cell using TA323910(ETS1 Antibody) at dilution 1/200
WB Suggested Anti-ETS1 Antibody Titration: 1.0ug/ml; Positive Control: HepG2 cell lysate
Human kidney
Human Pancrease
Rabbit Anti-ETS1 Antibody; Paraffin Embedded Tissue: Human alveolar cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Western blot analysis of extracts of various cell lines, using ETS1 antibody.
Immunohistochemistry of paraffin-embedded human stomach cancer using ETS1 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded rat lung using ETS1 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of U2OS cell using ETS1 antibody. Blue: DAPI for nuclear staining.
Host: Rabbit; Target Name: ETS1; Sample Tissue: Thyroid tumor lysates; Antibody Dilution: 1.0ug/ml
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06415SU-N | ETS1 antibody |
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 |
Mouse | IgG1 | 8A8 | Ascites | Hu | E, ICC/IF, P, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| AM06416SU-N | ETS1 antibody |
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 |
Mouse | IgG1 | 10D2 | Ascites | Hu | E, P, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP06524PU-N | ETS1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| AP55698PU-N | ETS1 pThr38 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP55698PU-S | ETS1 pThr38 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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