ETS2 antibody
Principal name
ETS2 antibody
Alternative names for ETS2 antibody
Protein C-ets-2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ETS2 antibody
ETS2 contains an ETS DNA-binding domain and belongs to the ETS family. ETS2 is a target of protein kinase C and upregulates GM-CSF. Ets2 and its targets play essential roles in endothelial cell function. Coexpression of Ets-2 and SRC-1 significantly associated with the rate of recurrence and HER expression in breast cancer. Overexpression of ETS2 is associated with human esophageal squamous cell carcinoma. ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Zabuawala, T., et al. Cancer Res. 70(4):1323-1333(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Shalaby, A.A., et al. Mod. Pathol. 22(8):996-1005(2009)
Sutter, W., et al. J Craniomaxillofac Surg 37(5):263-271(2009)
Staun-Ram, E., et al. Reprod. Biol. Endocrinol. 7, 135 (2009)
Human Breast; Breast; ETS2 Antibody - C-terminal region (P100751_P050) in Human Breast cells using Immunohistochemistry
Human 293T; WB Suggested Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; ETS2 Antibody - C-terminal region (P100751_P050) in Human 293T cells using Western Blot
Western Blot analysis of ETS2 expression in transfected 293T cell line (H00002114-T01) by ETS2 MaxPab polyclonal antibody.Lane 1: ETS2 transfected lysate(51.59 KDa).Lane 2: Non-transfected lysate.
human LCL, mouse brains; ETS2 antibody - middle region (ARP38592_P050) validated by WB using human LCL and mouse brains at 1:1000.; ETS2 antibody - middle region (ARP38592_P050) in human LCL, mouse brains cells using Western Blot
Human kidney; WB Suggested Anti-ETS2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human kidney; ETS2 antibody - middle region (ARP38592_P050) in Human kidney cells using Western Blot
Transfected 293T; WB Suggested Anti-ETS2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; ETS2 antibody - N-terminal region (ARP38591_P050) in Transfected 293T cells using Western Blot
Formalin fixed and paraffin embedded brain tissue reacted with ETS2 Antibody (Center) Cat.-No AP51471PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of ETS2 Antibody (Center) Cat.-No AP51471PU-N in MCF-7 cell line lysates (35ug/lane). ETS2 (arrow) was detected using the purified Pab.
Flow cytometric analysis of MCF-7 cells using ETS2 Antibody (Center) Cat.-No AP51471PU-N(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETS2 (RC207377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETS2.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505372)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505372)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505372)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505372)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETS2 (RC207377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETS2.
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-ETS2 monoclonal antibody.(1:200)
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-ETS2 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Figure from citation: Western Blot of ETS2 protein level by using anti-ETS2 antibody in MCECs. Dilution: 1:2000 View Citation
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505391)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505391)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505391)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505391)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505391)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505391)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505391)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505391)
Anti-ETS2 mouse monoclonal antibody (TA505391) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ETS2(RC207377).
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ETS2 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETS2 (RC207377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETS2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETS2 (RC207377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETS2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETS2 (RC207377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETS2.
WB Suggested Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T
Breast
WB Suggested Anti-ETS2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
ETS2 antibody - middle region validated by WB using human LCL and mouse brains at 1: 1000.
WB Suggested Anti-ETS2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human kidney
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA349947 | ETS2 antibody |
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| Rabbit Polyclonal Anti-ETS2 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| AP51471PU-N | ETS2 (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
 +2 additional images |
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