EVI1 antibody
Principal name
EVI1 antibody
Alternative names for EVI1 antibody
EVI-1, MECOM, Ecotropic virus integration site 1 protein homolog, MDS1 and EVI1 complex locus protein EVI1
Gene ID
2122 (MECOM)
Ncbi ID
9606, NP_001098547.3, NP_005232, NM_005241, NP_001098547, ACE87488.1, NM_001105078, NM_001105077, NM_001164000, NM_001163999, NM_004991
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of EVI1 antibody
- Kit Component:
- - KN210135G1, MECOM gRNA vector 1 in pCas-Guide vector
- KN210135G2, MECOM gRNA vector 2 in pCas-Guide vector
- KN210135D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Gomez-Benito, M., et al. Br. J. Cancer 103(8):1292-1296(2010)
Meyer, T.E., et al. PLoS Genet. 6 (8) (2010)
Goyama, S., et al. Int. J. Hematol. 91(5):753-757(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)
Haas, K., et al. Genes Chromosomes Cancer 47(4):288-298(2008).
Detection limit for recombinant GST tagged EVI1 is approximately 0.03ng/ml as a capture antibody.
Human Liver; EVI1 antibody - N-terminal region (ARP32265_P050) in Human Liver cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-EVI1 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; EVI1 antibody - N-terminal region (ARP32265_P050) in Human HepG2 cells using Western Blot
Human Brain; WB Suggested Anti-EVI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; EVI1 antibody - middle region (ARP58568_P050) in Human Brain cells using Western Blot
Human HeLa; WB Suggested Anti-EVI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; EVI1 antibody - N-terminal region (ARP58384_P050) in Human HeLa cells using Western Blot
Human kidney; WB Suggested Anti-EVI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human kidney; EVI1 antibody - middle region (ARP58566_P050) in Human kidney cells using Western Blot
Western blot analysis of EVI1 Antibody (N-term) Cat.-No AP51475PU-N in CEM cell line lysates (35ug/lane). This demonstrates the EVI1 antibody detected the EVI1 protein (arrow).
ELISA: EVI1 Antibody
ELISA: EVI1 Antibody
(2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-EVI1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
Human Liver
Western blot analysis of extracts of various cell lines, using MECOM antibody.
WB Suggested Anti-EVI1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human brain
WB Suggested Anti-EVI1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human kidney
WB Suggested Anti-EVI1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Hela cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332833 | EVI1 antibody |
||||||
| Rabbit anti-MECOM Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||