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Exonuclease 1 (EXO1) antibody

Principal name

Exonuclease 1 (EXO1) antibody

Alternative names for Exonuclease 1 (EXO1) antibody

Exonuclease I, EXOI, HEX1

SwissProt ID

Q9QZ11 (Mouse), Q9UQ84 (Human)

Gene ID

9156 (EXO1), 26909 (Exo1)

Ncbi ID

9606, NP_003677.4, 10090, NP_003677, NP_006018.3, NM_130398, NP_036142.2, NM_012012, NM_006027, NM_003686

Available reactivities

Hu (Human), Pl (Plant), Ms (Mouse)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ChIP assay (ChIP)

Background of Exonuclease 1 (EXO1) antibody

Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the mExo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/ hEXO1 gene to chromosome 1q42-q43. mExo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5'-3' double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The mismatch repair (MMR) system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MM. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins.

General readings

Kim JC, Roh SA, Yoon YS, Kim HC, Park IJ.
MLH3 and EXO1 alterations in familial colorectal cancer patients not fulfilling Amsterdam criteria.
Cancer Genet Cytogenet. 2007 Jul 15;176(2):172-4. No abstract available.
PMID: 17656264

EXO1 monoclonal antibody (M01), clone 1G12 Western Blot analysis of EXO1 expression in A-431 ( Cat # L015V1 ).

H00009156-M01

EXO1 polyclonal antibody ( Cat # PAB7442 ) ( 0.2 µg/ml ) staining of A-549 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7442

Detection limit for recombinant GST tagged EXO1 is approximately 1ng/ml as a capture antibody.

H00009156-M02

Western blot analysis of anti-EXO1 Antibody (N-term) (Cat.#AP12539PU-N) in NCI-H460 cell line lysates (35ug/lane). EXO1 (arrow) was detected using the purified Pab.

AP12539PU-N

Immunoprecipitation of EXO1 transfected lysate using anti-EXO1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with EXO1 MaxPab mouse polyclonal antibody (B01) (H00009156-B01).

H00009156-D01

Western blot (WB) analysis of Exo1 antibody (Cat.-No.: AP20554PU-N) in extracts from raw264.7 cells.

AP20554PU-N

Immunofluorescence analysis of A549 cells, using EXO1 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from A549 cells, using EXO1 Antibody. The lane on the right is treated with the synthesized peptide.

IHC Image of AP22963PU-NSkeletal muscle, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP22963PU-N

IHC Image of AP22963PU-NAdrenal, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP22963PU-N

Immunofluorescent analysis of EXO1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of EXO1 expression in Raw264.7 (A); A431 (B) whole cell lysates.

TA303326 (0.2µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303326

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA303326	Exonuclease 1 (EXO1) antibody
	Goat Polyclonal Antibody against EXO1
	Goat		Aff - Purified	Hu	WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
AP12539PU-N	Exonuclease 1 (EXO1) (N-term) antibody
	Rabbit	Ig	Purified	Hu	WB	0.4 ml / €415.00
						OriGene Technologies GmbH
AP20554PU-N	Exonuclease 1 (EXO1) antibody
	Rabbit		Aff - Purified	Hu, Ms	ICC/IF, WB	0.1 mg / €370.00
						OriGene Technologies GmbH

Lysates

Catalog No.
LY408989	EXO1 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR210902L2V	Lenti ORF particles, Exo1 (GFP-tagged) - Mouse exonuclease 1 (Exo1), 200 uL, >10^7 TU/mL
		200 µl / €1,670.00
		OriGene Technologies, Inc.

MR210902L1V	Lenti ORF particles, Exo1 (Myc-DDK-tagged) - Mouse exonuclease 1 (Exo1), 200 uL, >10^7 TU/mL
		200 µl / €1,500.00
		OriGene Technologies, Inc.

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